{"id":42788,"date":"2021-05-25T10:53:53","date_gmt":"2021-05-25T02:53:53","guid":{"rendered":"https:\/\/www.gene-optimal.com\/?p=42788"},"modified":"2023-12-05T13:17:51","modified_gmt":"2023-12-05T05:17:51","slug":"bacterial-dna-extraction-kit","status":"publish","type":"post","link":"https:\/\/www.gene-optimal.com\/bacterial-dna-extraction-kit\/","title":{"rendered":"\u7ec6\u83cc\u57fa\u56e0\u7ec4DNA\u63d0\u53d6\u8bd5\u5242\u76d2"},"content":{"rendered":"<p>\u6211\u4eec\u5df2\u63a8\u51fa\u4e00\u7cfb\u5217\u9488\u5bf9\u5206\u679d\u6746\u83cc\u7684\u5206\u5b50\u64cd\u4f5c\u5de5\u5177\u9176\uff0c\u4f46\u8fd8\u9700\u8981\u597d\u7528\u7684\u63d0\u53d6\u8bd5\u5242\u76d2\u6765\u914d\u5408\u3002\u5b9e\u9645\u5de5\u4f5c\u4e2d\u4e5f\u7684\u786e\u5e38\u5e38\u9047\u5230\u56e0\u4e3a\u57fa\u56e0\u7ec4\u7684\u6d53\u5ea6\u548c\u7eaf\u5ea6\u4e0d\u591f\u7b49\u95ee\u9898\u5bfc\u81f4\u5206\u5b50\u5b9e\u9a8c\u64cd\u4f5c\u4e0d\u591f\u5b8c\u7f8e\u6216\u5bfc\u81f4\u5b9e\u9a8c\u5931\u8d25\u3002\u4e3a\u89e3\u51b3\u8fd9\u4e9b\u5206\u5b50\u64cd\u4f5c\u5e38\u89c1\u7684\u95ee\u9898\uff0c\u6211\u4eec\u4e30\u5bcc\u4e86\u5206\u5b50\u64cd\u4f5c\u4ea7\u54c1\uff0c\u4e5f\u5c31\u662f\u6211\u4eec\u89e3\u51b3\u7ed3\u6838\u5206\u679d\u6746\u83cc\u5206\u5b50\u5b9e\u9a8c\u64cd\u4f5c\u7684\u91cd\u8981\u6b66\u5668\u2014\u2014\u6676\u8bfa\u5206\u679d\u6746\u83cc\u57fa\u56e0\u7ec4\u63d0\u53d6\u8bd5\u5242\u76d2\u3002<\/p>\n<p>\u5927\u5bb6\u90fd\u77e5\u9053\uff0c\u5206\u679d\u6746\u83cc\u5177\u6709\u8f83\u539a\u7ec6\u80de\u58c1\uff0c\u8102\u8d28\u542b\u91cf\u9ad8\uff0c\u5e38\u4f1a\u9047\u5230\u57fa\u56e0\u7ec4\u63d0\u53d6\u56f0\u96be\u7b49\u95ee\u9898\u3002\u6676\u8bfa\u4f5c\u4e3a\u4e00\u5bb6\u4e13\u4e1a\u4ece\u4e8b\u5206\u679d\u6746\u83cc\u9057\u4f20\u64cd\u4f5c\u7684\u516c\u53f8\uff0c\u9488\u5bf9\u5206\u679d\u6746\u83cc\u57fa\u56e0\u7ec4\u63d0\u53d6\u79ef\u7d2f\u4e86\u4e30\u5bcc\u7684\u5b9e\u8df5\u7ecf\u9a8c\uff0c\u4e5f\u5728\u5b9e\u8df5\u4e2d\u5bf9\u63d0\u53d6\u65b9\u6cd5\u4e0d\u65ad\u7684\u4f18\u5316\u548c\u63d0\u5347\uff0c\u5f62\u6210\u4e86\u6210\u719f\u7684\u89e3\u51b3\u65b9\u6848\uff0c\u5e76\u57fa\u4e8e\u6b64\u8bbe\u8ba1\u548c\u53d1\u5c55\u4e86\u4e00\u6b3e\u4e13\u95e8\u9488\u5bf9\u5206\u679d\u6746\u83cc\u57fa\u56e0\u7ec4\u63d0\u53d6\u7684\u8bd5\u5242\u76d2\uff0c\u4e3a\u5206\u679d\u6746\u83cc\u7684\u57fa\u56e0\u7ec4\u63d0\u53d6\u63d0\u4f9b\u66f4\u4fbf\u6377\u3001\u66f4\u9ad8\u6548\u3001\u66f4\u53ef\u9760\u7684\u65b9\u6cd5\u9009\u62e9\u3002<\/p>\n<section data-tools=\"135\u7f16\u8f91\u5668\" data-id=\"8\">\n<section data-brushtype=\"text\"><strong>\u7ec6\u83cc\u57fa\u56e0\u7ec4DNA\u63d0\u53d6\u8bd5\u5242\u76d2<\/strong><\/section>\n<\/section>\n<p>\u672c\u8bd5\u5242\u76d2\u9488\u5bf9\u7ec6\u80de\u58c1\u542b\u8f83\u539a\u8102\u8d28\u7684\u5206\u679d\u6746\u83ccDNA\u63d0\u53d6\u95ee\u9898\uff0c\u8bbe\u8ba1\u4e86<strong>\u4e13\u95e8\u9002\u7528\u4e8e\u5206\u679d\u6746\u83cc\u57fa\u56e0\u7ec4\u63d0\u53d6<\/strong>\u7684\u8bd5\u5242\u76d2\uff0c\u80fd\u591f\u5b8c\u7f8e\u89e3\u51b3\u5206\u679d\u6746\u83ccDNA\u63d0\u53d6\u56f0\u96be\u7684\u95ee\u9898\u3002\u8bd5\u5242\u76d2\u91c7\u7528\u53ef\u4ee5\u9ad8\u6548\u3001\u4e13\u4e00\u7ed3\u5408DNA\u7684\u79bb\u5fc3\u5438\u9644\u67f1\u548c\u4f18\u5316\u7684\u7f13\u51b2\u6db2\u7cfb\u7edf\uff0c\u4e0d\u4f46\u53ef\u4ee5\u5e94\u7528\u4e8e\u5206\u679d\u6746\u83cc\u57fa\u56e0\u7ec4\u7684\u9ad8\u6548\u63d0\u53d6\uff0c\u4e5f\u53ef\u7528\u4e8e\u5176\u4ed6\u7ec6\u83ccDNA\u7684\u63d0\u53d6\u3002<\/p>\n<p>\u8bd5\u5242\u76d2\u53ef\u4ece1-5 ml\u7ec6\u83cc\u57f9\u517b\u6db2\u4e2d\uff0c\u5feb\u901f\u63d0\u53d6\u8fbe<strong>10-20 \u03bcg\u9ad8\u7eaf\u5ea6<\/strong>\u7684\u57fa\u56e0\u7ec4DNA\uff0c\u6240\u5f97\u57fa\u56e0\u7ec4DNA\u53ef\u76f4\u63a5\u7528\u4f5cPCR\u6a21\u677f\u3001\u6d4b\u5e8f\u5efa\u5e93\u3001\u9176\u5207\u53ca\u5176\u4ed6\u7684\u5206\u5b50\u9057\u4f20\u64cd\u4f5c\u5b9e\u9a8c\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-42789\" src=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2021\/05\/\u7ec6\u83cc\u57fa\u56e0\u7ec4DNA\u63d0\u53d6\u8bd5\u5242\u76d2600.jpg\" alt=\"\" width=\"600\" height=\"338\" srcset=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2021\/05\/\u7ec6\u83cc\u57fa\u56e0\u7ec4DNA\u63d0\u53d6\u8bd5\u5242\u76d2600.jpg 600w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2021\/05\/\u7ec6\u83cc\u57fa\u56e0\u7ec4DNA\u63d0\u53d6\u8bd5\u5242\u76d2600-150x85.jpg 150w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2021\/05\/\u7ec6\u83cc\u57fa\u56e0\u7ec4DNA\u63d0\u53d6\u8bd5\u5242\u76d2600-300x169.jpg 300w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2021\/05\/\u7ec6\u83cc\u57fa\u56e0\u7ec4DNA\u63d0\u53d6\u8bd5\u5242\u76d2600-200x113.jpg 200w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><strong>\u4ea7\u54c1\u4fe1\u606f\uff1a<\/strong><\/p>\n<p>\u4e2d\u6587\u540d\u79f0\uff1a\u7ec6\u83cc\u57fa\u56e0\u7ec4DNA\u63d0\u53d6\u8bd5\u5242\u76d2<br \/>\n\u82f1\u6587\u540d\u79f0\uff1aBacterial DNA Extraction Kit<br \/>\n\u8d27\u53f7\uff1a60300K-50T<br \/>\n\u89c4\u683c\uff1a50T<br \/>\n\u8fd0\u8f93\u6761\u4ef6\uff1a\u5e38\u6e29<br \/>\n\u50a8\u5b58\u6761\u4ef6\uff1a\u6309\u6807\u7b7e\u63d0\u793a<\/p>\n<section data-role=\"paragraph\">&nbsp;<\/p>\n<p><strong>\u4ea7\u54c1\u5185\u5bb9\uff1a<\/strong><\/p>\n<p>DNA Columns<br \/>\n2 mL Collection Tubes<br \/>\nTE Buffer<br \/>\nLYS Buffer<br \/>\nEXT Buffer<br \/>\nPD Buffer (Add 10mL Isopropanol before use)<br \/>\nPW Buffer (Add 60 mL Ethanol before use)<br \/>\nElution Buffer<br \/>\nLysozyme<br \/>\nProteinase K Solution<br \/>\nRNase A<\/p>\n<p>&nbsp;<\/p>\n<p><strong>\u4ea7\u54c1\u7279\u70b9\uff1a<\/strong><\/p>\n<p>1\u3001\u9ad8\u8d28\u91cfDNA\u63d0\u53d6\uff1aDNA\u4ea7\u91cf\u5927\u3001\u5b8c\u6574\u6027\u597d\uff0c\u5373\u7528\u578bDNA\u3002<br \/>\n2\u3001\u5206\u679d\u6746\u83ccDNA\u63d0\u53d6\uff1a\u5b8c\u7f8e\u89e3\u51b3\u4e86\u5206\u679d\u6746\u83cc\u57fa\u56e0\u7ec4\u63d0\u53d6\u56f0\u96be\u95ee\u9898\u3002<br \/>\n3\u3001\u7b80\u5355\u5feb\u901f\uff1a\u53ef\u5feb\u901f\u5b8c\u6210\u9ad8\u8d28\u91cfDNA\u7684\u63d0\u53d6\u3002<br \/>\n4\u3001\u9002\u7528\u6027\u5e7f\uff1a\u540c\u65f6\u4e5f\u9002\u7528\u4e8e\u5176\u5b83\u7ec6\u83cc\u7684DNA\u63d0\u53d6\u3002<\/p>\n<\/section>\n<section data-role=\"paragraph\"><\/section>\n<section data-role=\"paragraph\"><\/section>\n<section data-role=\"paragraph\"><strong>\u6548\u679c\u5c55\u793a\uff1a<\/strong><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-42790\" src=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2021\/05\/pic.png\" alt=\"\" width=\"577\" height=\"295\" srcset=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2021\/05\/pic.png 577w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2021\/05\/pic-150x77.png 150w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2021\/05\/pic-300x153.png 300w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2021\/05\/pic-200x102.png 200w\" sizes=\"(max-width: 577px) 100vw, 577px\" \/><\/p>\n<p style=\"text-align: center;\">1\uff1a\u8fdb\u53e3\u57fa\u56e0\u7ec4\u63d0\u53d6\u8bd5\u5242\u76d2\uff1b2-10\uff1a\u6676\u8bfa\u57fa\u56e0\u7ec4\u63d0\u53d6\u8bd5\u5242\u76d2\uff1bM\uff1amaker<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-42791\" src=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2021\/05\/ee48180dee9ae1f3ea2b110bdc5d430.png\" alt=\"\" width=\"808\" height=\"94\" srcset=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2021\/05\/ee48180dee9ae1f3ea2b110bdc5d430.png 808w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2021\/05\/ee48180dee9ae1f3ea2b110bdc5d430-150x17.png 150w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2021\/05\/ee48180dee9ae1f3ea2b110bdc5d430-300x35.png 300w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2021\/05\/ee48180dee9ae1f3ea2b110bdc5d430-768x89.png 768w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2021\/05\/ee48180dee9ae1f3ea2b110bdc5d430-800x94.png 800w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2021\/05\/ee48180dee9ae1f3ea2b110bdc5d430-200x23.png 200w\" sizes=\"(max-width: 808px) 100vw, 808px\" \/><\/p>\n<p>\u5bf91mL\u5206\u679d\u6746\u83cc\u83cc\u6db2\uff08\u83cc\u91cf1 X 10<sup>8<\/sup>\uff09\u6309\u8bf4\u660e\u4e66\u65b9\u6cd5\u8fdb\u884cDNA\u63d0\u53d6\uff0c\u6eb6\u4e8e50uL\u65e0\u83cc\u6c34\u4e2d\uff0c\u5bf9DNA\u63d0\u53d6\u6548\u679c\u8fdb\u884c\u7535\u6cf3\uff08\u70b9\u6837\u91cf2uL)\u53caDNA\u6d53\u5ea6\u3001\u7eaf\u5ea6\u68c0\u6d4b\u3002\u7ed3\u679c\u663e\u793a\uff0cDNA\u5177\u6709\u8f83\u9ad8\u7684\u5b8c\u6574\u6027\u3001\u6d53\u5ea6\u548c\u7eaf\u5ea6\uff0cDNA\u603b\u91cf\u53ef\u8fbe1ug\uff0c\u76f8\u6bd4\u4e8e\u8fdb\u53e3\u7684\u7ec6\u83cc\u57fa\u56e0\u7ec4\u63d0\u53d6\u8bd5\u5242\u76d2\u4ea7\u7387\u66f4\u9ad8\u3002<\/p>\n<p>\u6b64\u5916\uff0c\u6211\u4eec\u4e5f\u5c06\u9646\u7eed\u63a8\u51fa\u4e00\u7cfb\u5217\u5206\u5b50\u64cd\u4f5c\u5e38\u7528\u8bd5\u5242\u76d2\u4ea7\u54c1\uff0c\u5982\u8d28\u7c92\u63d0\u53d6\u3001\u6838\u9178\u3001\u86cb\u767dMaker\u7b49\uff0c\u52a9\u529b\u5927\u5bb6\u5206\u5b50\u64cd\u4f5c\u5b9e\u9a8c\u66f4\u52a0\u8f7b\u677e\u3001\u5feb\u6377\uff0c\u656c\u8bf7\u671f\u5f85\uff01<\/p>\n<p>&nbsp;<\/p>\n<p><strong>\u5de5\u5177\u9176\u7cfb\u5217\u4ea7\u54c1\uff1a<\/strong><\/p>\n<blockquote class=\"wp-embedded-content\" data-secret=\"Nl7WDnu4wY\"><p><a href=\"https:\/\/www.gene-optimal.com\/polymerase_ligase_cloning_kit\/\">\u5927\u63ed\u79d8\uff01\uff01\uff01\u9ad8GC\u57fa\u56e0\u7684\u5206\u5b50\u9057\u4f20\u64cd\u4f5c\u79d8\u8bc0<\/a><\/p><\/blockquote>\n<p><iframe class=\"wp-embedded-content\" sandbox=\"allow-scripts\" security=\"restricted\" style=\"position: absolute; clip: rect(1px, 1px, 1px, 1px);\" title=\"\u300a\u5927\u63ed\u79d8\uff01\uff01\uff01\u9ad8GC\u57fa\u56e0\u7684\u5206\u5b50\u9057\u4f20\u64cd\u4f5c\u79d8\u8bc0\u300b\u2014\u4e0a\u6d77\u6676\u8bfa\u751f\u7269\u79d1\u6280\u6709\u9650\u516c\u53f8\" src=\"https:\/\/www.gene-optimal.com\/polymerase_ligase_cloning_kit\/embed\/#?secret=Nl7WDnu4wY\" data-secret=\"Nl7WDnu4wY\" width=\"600\" height=\"338\" frameborder=\"0\" marginwidth=\"0\" marginheight=\"0\" scrolling=\"no\"><\/iframe><\/p>\n<p>&nbsp;<\/p>\n<\/section>\n","protected":false},"excerpt":{"rendered":"<p>\u6211\u4eec\u5df2\u63a8\u51fa\u4e00\u7cfb\u5217\u9488\u5bf9\u5206\u679d\u6746\u83cc\u7684\u5206\u5b50\u64cd\u4f5c\u5de5\u5177\u9176\uff0c\u4f46\u8fd8&#8230;<\/p>\n","protected":false},"author":1,"featured_media":42793,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[95],"tags":[],"_links":{"self":[{"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/posts\/42788"}],"collection":[{"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/comments?post=42788"}],"version-history":[{"count":7,"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/posts\/42788\/revisions"}],"predecessor-version":[{"id":44956,"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/posts\/42788\/revisions\/44956"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/media\/42793"}],"wp:attachment":[{"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/media?parent=42788"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/categories?post=42788"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/tags?post=42788"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}