{"id":42673,"date":"2020-11-26T14:03:53","date_gmt":"2020-11-26T06:03:53","guid":{"rendered":"https:\/\/www.gene-optimal.com\/?p=42673"},"modified":"2023-12-05T13:17:56","modified_gmt":"2023-12-05T05:17:56","slug":"sciad-est12","status":"publish","type":"post","link":"https:\/\/www.gene-optimal.com\/sciad-est12\/","title":{"rendered":"\u6676\u8bfa\u52a9\u529b\u6b66\u6c49\u5927\u5b66\u79d1\u7814\u56e2\u961f\u5728science\u5b50\u520a\u53d1\u8868\u6587\u7ae0\uff01"},"content":{"rendered":"<p><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-42674\" src=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201101.png\" alt=\"\" width=\"1192\" height=\"611\" srcset=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201101.png 1192w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201101-150x77.png 150w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201101-300x154.png 300w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201101-768x394.png 768w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201101-1024x525.png 1024w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201101-200x103.png 200w\" sizes=\"(max-width: 1192px) 100vw, 1192px\" \/>2020\u5e7410\u670821\u65e5\uff0c\u6b66\u6c49\u5927\u5b66\u7ae0\u6653\u8054\u3001\u6bb7\u96f7\u8001\u5e08\u4f5c\u4e3a\u5171\u540c\u901a\u8baf\u4f5c\u8005\u5728 Science Advances \u5728\u7ebf\u53d1\u8868\u6700\u65b0\u7814\u7a76\u6210\u679c \u201cMycobacterial EST12 activates a RACK1\u2013NLRP3\u2013gasdermin D pyroptosis\u2013IL-1\u03b2 immune pathway\u201d\uff0c\u8be5\u7814\u7a76\u9274\u5b9a\u4e86\u4e00\u79cd\u4ece <em>M. tb<\/em> H37Rv \u5206\u6ccc\u7684\u7ec6\u80de\u7126\u4ea1\u8bf1\u5bfc\u86cb\u767d EST12\uff08Rv1579c\uff09\u3002<\/span><\/p>\n<p><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\"><strong>\u6676\u8bfa\u751f\u7269\u4e3a\u672c\u7814\u7a76\u6784\u5efa\u4e86EST12\u6572\u9664\u83cc\u682a\u5e76\u63d0\u4f9b\u76f8\u5173\u6280\u672f\u652f\u6301\uff01\u611f\u8c22\u6b66\u6c49\u5927\u5b66\u7ae0\u6653\u8054\u3001\u6bb7\u96f7\u8001\u5e08\u7814\u7a76\u56e2\u961f\u5bf9\u6676\u8bfa\u751f\u7269\u7684\u4fe1\u4efb\u548c\u5927\u529b\u652f\u6301\uff01<\/strong><\/span><\/p>\n<p><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-42675\" src=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201102.png\" alt=\"\" width=\"1053\" height=\"355\" srcset=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201102.png 1053w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201102-150x51.png 150w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201102-300x101.png 300w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201102-768x259.png 768w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201102-1024x345.png 1024w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201102-200x67.png 200w\" sizes=\"(max-width: 1053px) 100vw, 1053px\" \/><\/span><\/p>\n<p><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\">\u5728\u5206\u4eab\u559c\u8baf\u7684\u540c\u65f6\uff0c\u4e5f\u8ddf\u5927\u5bb6\u5206\u4eab\u4e00\u4e0b\u8fd9\u7bc7\u5f71\u54cd\u56e0\u5b50\u4e3a13\u7684\u6587\u7ae0\uff01<\/span><\/p>\n<p>&nbsp;<\/p>\n<section><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\"><strong>\u5bfc\u8bfb\uff1a<\/strong><\/span><\/section>\n<p><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\">\u7ec6\u80de\u7126\u4ea1\u662f\u4e00\u79cd\u7a0b\u5e8f\u6027\u7ec6\u80de\u6b7b\u4ea1\u7684\u708e\u6027\u5f62\u5f0f\uff0c\u4e0e\u6d88\u9664\u75c5\u539f\u4f53\u611f\u67d3\u6709\u5173\u3002\u8be5\u6587\u7ae0\u4ecb\u7ecd\u4e86\u4e00\u79cd\u8bf1\u5bfc\u5de8\u566c\u7ec6\u80de\u7126\u4ea1\u7684\u7ed3\u6838\u86cb\u767d<strong>EST12<\/strong>\u3002EST12\u7ed3\u5408\u5de8\u566c\u7ec6\u80de\u4e2d\u6d3b\u5316\u7684C\u6fc0\u91761\uff08RACK1\uff09\u7684\u53d7\u4f53\uff0cEST12-RACK1\u590d\u5408\u4f53\u52df\u96c6\u53bb\u6cdb\u7d20\u5316\u9176UCHL5\u6765\u4fc3\u8fdbNLRP3\u7684K48\u8fde\u63a5\u53bb\u6cdb\u7d20\u5316\uff0c\u968f\u540e\u5bfc\u81f4NLRP3\u2013caspase-1\/11\u2013GSDMD\u2013IL-1\u03b2\u514d\u75ab\u8fc7\u7a0b\u3002EST12\u6676\u4f53\u7ed3\u6784\u5206\u6790\u8868\u660e\uff0c\u6c28\u57fa\u9178Y80\u662fRACK1\u7684\u5173\u952e\u7ed3\u5408\u4f4d\u70b9\u3002<strong>EST12\u6572\u9664\u83cc\u682a\u5728\u4f53\u5185\u6216\u4f53\u5916MTB\u611f\u67d3\u4e2d\u90fd\u663e\u793a\u51fa\u4e86\u9ad8\u6613\u611f\u6027\u3002<\/strong>\u8be5\u7814\u7a76\u9996\u6b21\u8bc1\u660e\u4e86RACK1\u4f5c\u4e3a\u75c5\u539f\u4f53\u7684\u5185\u6e90\u5bbf\u4e3b\u611f\u5e94\u86cb\u767d\uff0c\u5e76\u4e14EST12-RACK1\u8bf1\u5bfc\u7684\u7ec6\u80de\u7126\u4ea1\u5728<em>M.tb<\/em>\u8bf1\u5bfc\u7684\u514d\u75ab\u4e2d\u8d77\u5173\u952e\u4f5c\u7528\u3002<\/span><\/p>\n<section><\/section>\n<section><\/section>\n<section><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\"><strong>\u7814\u7a76\u5185\u5bb9\uff1a<\/strong><\/span><\/section>\n<section><\/section>\n<section><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\">1\u3001RD\u533a\u7f16\u7801\u86cb\u767d\u7b5b\u9009\uff1a<\/span><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\">\u5bf940\u79cdRD\u533a\uff08RD1-3\u300111-14\uff09\u91cd\u7ec4\u86cb\u767d\u8fdb\u884c\u7b5b\u9009\uff08\u5de8\u566c\u7ec6\u80de\u7126\u78f7\u9178\u76f8\u5173\u86cb\u767d\uff09\uff0c\u901a\u8fc7LDH\u91ca\u653e\u5b9e\u9a8c\u7ec6\u80de\u6bd2\u6027\u5206\u6790\uff0c\u53d1\u73b0EST12\u5bf9\u5de8\u566c\u7ec6\u80de\u6709\u663e\u8457\u7126\u89e3\u4f5c\u7528\u3002<\/span><\/section>\n<section><\/section>\n<section data-role=\"list\"><\/section>\n<section data-role=\"list\"><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\">2\u3001\u83cc\u682a\u6784\u5efa\uff1a<\/span><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\"><strong>\u6784\u5efa<em>M. tb<\/em>\u00a0H37Rv \u0394EST12\uff08Rv1579c\u6572\u9664\u83cc\u682a\uff09<\/strong>\u3001BCG-EST12\uff08Rv1579c\u8fc7\u8868\u8fbe\u83cc\u682a\uff09\u3001<em>M. smeg<\/em>\u2013EST12\uff08Rv1579c\u8fc7\u8868\u8fbe\u83cc\u682a\uff09\u3002<\/span><\/section>\n<section data-role=\"list\"><\/section>\n<section data-role=\"list\"><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\">3\u3001\u5c0f\u9f20\u57fa\u56e0\u6572\u9664\u6a21\u578b\uff1a<\/span><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\">NLRP3\u2212\/\u2212\u3001GSDMD\u2212\/\u2212\u3001caspase-1\/11\u2212\/\u2212\u5c0f\u9f20\u3001RACK1\u0394M\u03a6\u3002<\/span><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\">4\u3001\u68c0\u6d4b\u5185\u5bb9\uff1a<\/span><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\">LDH\uff08\u4e73\u9178\u8131\u6c22\u9176\uff09\u91ca\u653e\u5b9e\u9a8c\u68c0\u6d4b\u7ec6\u80de\u6bd2\u6027\u3001ELISA\u68c0\u6d4b\u7ec6\u80de\u56e0\u5b50\u5206\u6ccc\u3001WB\u68c0\u6d4b\u3001\u514d\u75ab\u6c89\u6dc0\uff08IP\uff09\u3001pull down\u3001\u8d28\u8c31\u3001EST12\u6676\u4f53\u7ed3\u6784\u3001\u5171\u805a\u7126\u663e\u5fae\u955c\u3001\u5c0f\u9f20\u7ed3\u6838\u611f\u67d3\u6a21\u578b\u7b49\u3002<\/span><\/section>\n<section><\/section>\n<section><\/section>\n<section><\/section>\n<section><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\"><strong>\u5b9e\u9a8c\u601d\u8def\uff1a<\/strong><\/span><\/section>\n<section><\/section>\n<section><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-42676\" src=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201103-3.jpg\" alt=\"\" width=\"946\" height=\"1608\" srcset=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201103-3.jpg 946w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201103-3-88x150.jpg 88w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201103-3-176x300.jpg 176w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201103-3-768x1305.jpg 768w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201103-3-602x1024.jpg 602w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201103-3-200x340.jpg 200w\" sizes=\"(max-width: 946px) 100vw, 946px\" \/><\/span><\/section>\n<section><\/section>\n<section>\n<section><\/section>\n<section><\/section>\n<section><\/section>\n<section><\/section>\n<section><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\"><strong>\u7814\u7a76\u7ed3\u679c\uff1a<\/strong><\/span><\/section>\n<section><\/section>\n<section><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\"><strong>1\u3001EST12\uff08Rv1579c\uff09\u8bf1\u5bfcGSDMD\u4ecb\u5bfc\u7684\u5de8\u566c\u7ec6\u80de\u7126\u4ea1<\/strong><\/span><\/section>\n<p><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\">\u8868\u8fbe\u7eaf\u531640\u79cdRD\u533a\u7f16\u7801\u86cb\u767d\uff0c\u901a\u8fc7LDH\u91ca\u653e\u5b9e\u9a8c\uff0c\u53d1\u73b0EST12\uff08Rv1579c\uff09\u5177\u6709\u660e\u663e\u7684\u5de8\u566c\u7ec6\u80de\u6bd2\u6027\u4f5c\u7528\u3002EST12\u86cb\u767d\u5bfc\u81f4\u5c0f\u9f20\u8179\u8154\u5de8\u566c\u7ec6\u80de\uff08\u56fe1A\u548cB\uff09\u3001\u4eba\u5355\u6838\u7ec6\u80deTHP-1\uff08\u56fe1C\uff09\u548c\u5c0f\u9f20\u9aa8\u9ad3\u6765\u6e90\u5de8\u566c\u7ec6\u80de\uff08BMDMs\uff09\u6b7b\u4ea1\uff08\u56fe1D\uff09\u3002\u7ed3\u679c\u8868\u660e\uff0cEST12\u5bf9\u5de8\u566c\u7ec6\u80de\u5177\u6709\u8f83\u5f3a\u7684\u6bd2\u6027\u3002<\/span><\/p>\n<\/section>\n<p><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-42677\" src=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201104.jpg\" alt=\"\" width=\"1050\" height=\"1066\" srcset=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201104.jpg 1050w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201104-148x150.jpg 148w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201104-295x300.jpg 295w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201104-768x780.jpg 768w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201104-1009x1024.jpg 1009w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201104-200x203.jpg 200w\" sizes=\"(max-width: 1050px) 100vw, 1050px\" \/><\/span><\/p>\n<p style=\"text-align: center;\"><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\">\u56fe1 \u5de8\u566c\u7ec6\u80de\u7126\u4ea1\u8bf1\u5bfc\u86cb\u767dEST12\u7684\u9274\u5b9a<\/span><\/p>\n<p><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\">A-D\uff1a\u5206\u522b\u7528EST12\u548cRv1577\u5904\u7406\u5c0f\u9f20\u8179\u8154\u5de8\u566c\u7ec6\u80de\uff08A\u3001B\uff09\u3001PMA\u5206\u5316\u7684THP1\u7ec6\u80de\uff08C\uff09\u3001\u5c0f\u9f20\u9aa8\u9ad3\u5de8\u566c\u7ec6\u80deBMDMS\uff08D\uff09\uff0c\u91c7\u7528LDH\u91ca\u653e\u6cd5\u5206\u6790\u3002E\uff1aWB\u68c0\u6d4b2\u03bc\uff2d EST12\u6216RV1577\u5904\u7406\u5de8\u566c\u7ec6\u80deGSDMD\u6d3b\u5316\uff1bF\uff1aELISA\u68c0\u6d4bIL-1\u03b2\u5206\u6ccc\uff1bG\uff1aWB\u68c0\u6d4bH37Rv\u548cH37Rv\u0394EST12\u611f\u67d3BMDMs\u7ec6\u80de\u540ecaspase-1\/GSDMD\u6d3b\u6027\uff1bH\uff1aLDH\u91ca\u653e\u5b9e\u9a8c\u68c0\u6d4b\u7ec6\u80de\u6bd2\u6027\uff1bI\uff1aELISA\u68c0\u6d4bIL-1\u03b2\u5206\u6ccc\uff1bJ\uff1aWB\u68c0\u6d4bBCG\u548cBCG-EST12\u611f\u67d3BMDMs\u7ec6\u80de\u540ecaspase-1\/GSDMD\u6d3b\u6027\uff1bK\uff1aLDH\u91ca\u653e\u5b9e\u9a8c\u68c0\u6d4b\u7ec6\u80de\u6bd2\u6027\uff0cELISA\u68c0\u6d4bIL-1\u03b2\u5206\u6ccc\u3002<\/span><\/p>\n<p>&nbsp;<\/p>\n<p><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\">\u4e3a\u786e\u5b9aGSDMD\u548cGSDME\u662f\u5426\u53c2\u4e0eEST12\u8bf1\u5bfc\u7684\u7ec6\u80de\u6b7b\u4ea1\uff0c2\u03bc\uff2d EST12\u523a\u6fc0\u5c0f\u9f20\u8179\u8154\u5de8\u566c\u7ec6\u80de\u4e0d\u540c\u65f6\u95f4\uff0c\u68c0\u6d4bGSDMD\u548cGSDME\u6d3b\u6027\u3002\u53d1\u73b02\u03bc\uff2d EST12\u523a\u6fc0\u5c0f\u9f20\u8179\u8154\u5de8\u566c\u7ec6\u80de0.5\u5c0f\u65f6GSDMD\u5168\u957f\u3001GSDMD N\u7aef\u548cC\u7aef\u88ab\u68c0\u6d4b\u5230\uff0ccaspase-1 P20\u7247\u6bb5\u88c2\u89e3\u6d3b\u6027\u589e\u5f3a\uff08\u56fe1E\uff09\uff0c\u5de8\u566c\u7ec6\u80deIL-1\u03b2\u5206\u6ccc\u589e\u52a0\uff081F\uff09\uff0cEST12\u8fdb\u4e00\u6b65\u5904\u7406\u4ebaTHP-1\u5355\u6838\u7ec6\u80de\u6765\u6e90\u5de8\u566c\u7ec6\u80de\uff0c\u53d1\u73b0caspase-1\u548cGSDMD\u88abEST12\u88c2\u89e3\u3002<\/span><\/p>\n<p><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\">\u68c0\u6d4bH37Rv\u548cH37Rv\u0394EST12\u611f\u67d3\u5de8\u566c\u7ec6\u80decaspase-1\u548cGSDMD\u6d3b\u6027\uff0cH37Rv\u2206EST12\u7ec4\u4e0eH37Rv\u7ec4\u5728\u611f\u67d3\u540e1\u5c0f\u65f6\u5185\u65e0\u660e\u663e\u53d8\u5316\u3002\u5728\u523a\u6fc06h\u540e\uff0cH37Rv\u5728BMDMs\u4e2d\u8bf1\u5bfc\u4e86\u66f4\u591a\u88c2\u89e3\u7684caspase-1 p20\u548cGSDMD-N\u672b\u7aef\u7247\u6bb5\uff08\u56fe1G\uff09\u3002\u4e0e\u4e4b\u7c7b\u4f3c\uff0cH37Rv\u2206EST12\u7ec4LDH\u91ca\u653e\u548cIL-1\u03b2\u5206\u6ccc\u4f4e\u4e8eH37Rv\u7ec4\uff08\u56fe1H\u3001I\uff09\u3002\u7ed3\u679c\u8868\u660e\uff0cH37Rv\u2206EST12\u7ec4\u9020\u6210\u7684\u708e\u6027\u7126\u4ea1\u5c11\u4e8eH37Rv\u7ec4\u3002\u5728BMDMs\u4e2d\uff0c\u4e0eBCG\u76f8\u6bd4BCG-EST12\u6301\u7eed\u8bf1\u5bfc\u66f4\u591a\u7684caspase-1 p20\u548cGSDMD-N\u672b\u7aef\u7247\u6bb5\uff08\u56fe1J\uff09\uff0c\u7ec6\u80de\u6bd2\u6027\u548cIL-1\u5206\u6ccc\u91cf\u66f4\u9ad8\uff08\u56fe1K\uff09\u3002\u7ed3\u679c\u8868\u660e\uff0c\u91cd\u7ec4BCG-EST12\u8f83BCG\u8bf1\u5bfcIL-1\u03b2\u8868\u8fbe\u91cf\u548c\u7ec6\u80de\u6bd2\u6027\u663e\u8457\u5347\u9ad8\u3002<\/span><\/p>\n<p><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\"><strong>\u7ed3\u679c\u5c0f\u7ed3\uff1aEST12\u901a\u8fc7GSDMD\u4ecb\u5bfc\u5de8\u566c\u7ec6\u80de\u7126\u4ea1\u548c\u708e\u6027\u7ec6\u80de\u56e0\u5b50IL-1\u5206\u6ccc\u3002<\/strong><\/span><\/p>\n<section><\/section>\n<section><\/section>\n<section><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\"><strong>2\u3001EST12\u4e0eRACK1\u76f8\u4e92\u4f5c\u7528\u8bf1\u5bfc\u5de8\u566c\u7ec6\u80de\u708e\u6027\u7126\u4ea1<\/strong><\/span><\/section>\n<p><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\">\u4e3a\u68c0\u6d4b\u7ec6\u80de\u4e2d\u4e0eEST12\u76f8\u4e92\u4f5c\u7528\u86cb\u767d\uff0c\u4f7f\u7528pull down\u5b9e\u9a8c\u548c\u8d28\u8c31\u68c0\u6d4b\uff0c\u7ed3\u679c\u53d1\u73b0RACK1\u86cb\u767d\u53ef\u80fd\u662f\u4e0eEST12\u7684\u7ed3\u5408\u86cb\u767d\uff0c\u514d\u75ab\u5370\u8ff9\u8fdb\u4e00\u6b65\u9a8c\u8bc1\u4e86\u8fd9\u4e2a\u7ed3\u679c\uff08\u56fe2A\uff09\u3002\u5c06EST12-\u7eff\u8272\u8367\u5149\u548cRACK1-\u7ea2\u8272\u8367\u5149\u86cb\u767d\u8868\u8fbe\u8d28\u7c92\u8f6c\u67d3\u5230\u5de8\u566c\u7ec6\u80deRAW264.7\u4e2d\uff0c\u5171\u805a\u7126\u8367\u5149\u663e\u5fae\u955c\u663e\u793aEST12\u548cRACK1\u5728\u7ec6\u80de\u8d28\u4e2d\u5b9a\u4f4d\uff0c\u4ea7\u751f\u9ec4\u8272\uff08\u56fe2B\uff09\u3002qPCR\u548cWB\u5206\u6790\u7ed3\u679c\u663e\u793a\uff0cEST12\u523a\u6fc0\u5de8\u566c\u7ec6\u80deRACK1\u7684\u8868\u8fbe\uff0c\u57286h\u65f6\u8fbe\u5230\u5cf0\u503c\uff0c\u7136\u540e\u4e0b\u964d\uff08\u56fe2C\uff09,\u63a8\u6d4bEST12\u5728\u65e9\u671f\u4fc3\u8fdbRACK1\u7684\u8868\u8fbe\u3002<\/span><\/p>\n<p><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-42678\" src=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201105.jpg\" alt=\"\" width=\"900\" height=\"847\" srcset=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201105.jpg 900w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201105-150x141.jpg 150w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201105-300x282.jpg 300w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201105-768x723.jpg 768w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201105-200x188.jpg 200w\" sizes=\"(max-width: 900px) 100vw, 900px\" \/><\/span><\/p>\n<p style=\"text-align: center;\"><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\">\u56fe2 EST12\u4e0eRACK1\u76f8\u4e92\u4f5c\u7528\u8bf1\u5bfc\u5de8\u566c\u7ec6\u80de\u708e\u6027\u7126\u4ea1<\/span><\/p>\n<p><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\">A\uff1aRAW264.7\u7ec6\u80de\u8fdb\u884cEST12-His pull-down\u5b9e\u9a8c\uff0cWB\u5206\u6790\uff1bB\uff1a\u5171\u805a\u7126\u663e\u5fae\u955c\u5206\u6790\u5206\u522b\u8f6c\u67d3pEGFP-C1-EST12\u548cpAsRED2-C1-RACK1\u7684RAW264.7\u7ec6\u80de\uff1bC\uff1aWB\u548cqPCR\u5206\u67902\u03bc\uff2d EST12\u523a\u6fc0\u7684RAW264.7\u7ec6\u80de\uff1bD\u3001E\uff1aLDH\u3001ELISA\u5206\u67902\u03bc\uff2d EST12\u5904\u7406WT\u548cRACK1\u2212\/\u2212\u8179\u8154\u5de8\u566c\u7ec6\u80de\u4e0a\u6e05\uff1bF\uff1aWB\u68c0\u6d4b\u7ec6\u80de\u88c2\u89e3\u6db2\u4e2dGSDMD\u548ccaspase-1\uff1bG\uff1a2\u03bc\uff2d EST12\u5904\u7406\u548cPI\u67d3\u8272WT\u3001RACK1\u2212\/\u2212\u5de8\u566c\u7ec6\u80de\uff1bE-J\uff1aLDH\u3001ELISA\u5206\u6790H37Rv\u548cH37Rv\u0394EST12\u3001BCG\u548cBCG-EST12\u3001<em>M. smeg<\/em>\u548c<em>M. smeg<\/em>-EST12\u611f\u67d3WT\u3001RACK1\u2212\/\u2212BMDMs\u3002<\/span><\/p>\n<p>&nbsp;<\/p>\n<p><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\">\u7814\u7a76EST12\u4e0eRACK1\u5728\u5de8\u566c\u7ec6\u80de\u4e2d\u76f8\u4e92\u4f5c\u7528\uff0c2\u03bcM EST12\u5206\u522b\u523a\u6fc0WT\u548cRACK1\u2212\/\u2212\u8179\u8154\u5de8\u566c\u7ec6\u80de\uff0c\u4e0eWT\u5de8\u566c\u7ec6\u80de\u76f8\u6bd4\uff0cRACK1\u2212\/\u2212\u8179\u8154\u5de8\u566c\u7ec6\u80de\u4e2dEST12\u8bf1\u5bfc\u7684\u7ec6\u80de\u6bd2\u6027\uff08\u56fe2D\uff09\u548cIL-1\u5206\u6ccc\uff08\u56fe2E\uff09\u663e\u8457\u964d\u4f4e\uff0cGSDMD N\u7247\u6bb5\u548ccaspase-1 p10\u88c2\u89e3\u88ab\u5f3a\u70c8\u6291\u5236\uff08\u56fe2F\uff09\u3002\u5b9e\u65f6\u5171\u7126\u663e\u5fae\u955c\u5206\u6790\u663e\u793a\uff0cEST12\u5bfc\u81f4\u8179\u8154\u5de8\u566c\u7ec6\u80de\u5448\u73b0\u5178\u578b\u7684\u7ec6\u80de\u7126\u4ea1\u5f62\u6001\uff1a\u8d28\u819c\u7834\u88c2\uff0c\u591a\u4e2a\u6ce1\u72b6\u7a81\u8d77\uff0c\u7ec6\u80de\u6838\u5185\u5448\u201c\u714e\u86cb\u201d\u72b6\uff08\u56fe2G\uff09\u3002H37Rv\u0394EST12\u611f\u67d3WT BMDMs\u548cH37Rv\u611f\u67d3RACK1\u2212\/\u2212BMDMs\u7684\u7ec6\u80de\u51cb\u4ea1\u548cIL-1\u03b2\u5206\u6ccc\u65e0\u533a\u522b\uff08\u56fe2H\uff09\u3002BCG-EST12\u548cBCG\u611f\u67d3RACK1\u2212\/\u2212BMDMs\u7684\u7ec6\u80de\u6bd2\u6027\u548cIL-1\u03b2\u5206\u6ccc\u65e0\u660e\u663e\u533a\u522b\uff08\u56fe2I\uff09\u3002<em>M. smeg<\/em>\u548c<em>M. smeg<\/em>-EST12\u5b9e\u9a8c\u7ec4\u4e5f\u662f\u540c\u6837\u7ed3\u679c\uff08\u56fe2J\uff09\u3002<\/span><\/p>\n<p><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\"><strong>\u7ed3\u679c\u5c0f\u7ed3\uff1aEST12\u901a\u8fc7RACK1\u5f15\u8d77\u5de8\u566c\u7ec6\u80de\u708e\u6027\u7126\u4ea1\u3002<\/strong><\/span><\/p>\n<section><\/section>\n<section><\/section>\n<section><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\"><strong>3\u3001EST12\u86cb\u767dC\u7aef\u548cY80\u662f\u4e0eRACK1\u7ed3\u5408\u7684\u5173\u952e\u4f4d\u70b9<\/strong><\/span><\/section>\n<p><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\">\u5bf9EST12-RACK1\u7ed3\u6784\u9884\u6d4b\uff08\u56fe3B\uff09\uff0cEST12\u4e0eRACK1\u7684\u76f8\u4e92\u4f5c\u7528\u53ef\u80fd\u4f9d\u8d56\u4e8eEST12\u7684\u7b2c\u4e09\u4e2a\u87ba\u65cb\u72b6\u7ed3\u6784\uff08E55\u3001F76\u3001Y80\uff09\u3002\u91cd\u7ec4\u86cb\u767d\uff08\u56fe3C\uff09\u5bf9RAW264.7\u7ec6\u80de\u88c2\u89e3\u7269\u8fdb\u884cpull down\u5b9e\u9a8c\uff0c\u7136\u540e\u514d\u75ab\u5370\u8ff9\u5206\u6790\uff0c\u4e0eEST12\u548cEST12-D2\u86cb\u767d\u76f8\u6bd4\uff0cEST12-D1\uff08C\u7aef\u7f3a\u5931\uff09\u4e0eRACK1\u51e0\u4e4e\u6ca1\u6709\u76f8\u4e92\u4f5c\u7528\uff08\u56fe3D\uff09\uff0cY80A\u548cF76A\u4e0eRACK1\u7684\u7ed3\u5408\u4f5c\u7528\u660e\u663e\u4f4e\u4e8eEST12\uff08\u56fe3E\uff09\u3002\u4e0eEST12\u76f8\u6bd4\uff0cEST12-D1\u7684\u7ec6\u80de\u6bd2\u6027\u4f5c\u7528\u4e5f\u660e\u663e\u51cf\u5f31\uff08\u56fe3F\u3001G\uff09\uff0cEST12-E55A\u3001F76A\u3001Y80A\u8bf1\u5bfc\u7684\u7ec6\u80de\u6bd2\u6027\u4f5c\u7528\u4e0b\u964d\uff0c\u5176\u4e2dEST12-Y80A\u6700\u4e3a\u660e\u663e\uff08\u56fe3H\u3001I\uff09\u3002\u540c\u6837\uff0c<em>M. smeg<\/em>-EST1-D1\u548c<em>M. smeg<\/em>-EST12-Y80A\u611f\u67d3\u5f15\u8d77\u7684\u7ec6\u80de\u7126\u4ea1\u8f83<em>M. smeg<\/em>-EST12\u51cf\u5c11\uff08\u56fe3J\u3001K\uff09\u3002<\/span><\/p>\n<p><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\"><strong>\u7ed3\u679c\u5c0f\u7ed3\uff1aEST12 C\u7aef\u7684Y80\u662fRACK1\u5f15\u8d77\u7126\u4ea1\u7684\u5173\u952e\u7ed3\u5408\u4f4d\u70b9\u3002<\/strong><\/span><\/p>\n<p><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-42679\" src=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201106.jpg\" alt=\"\" width=\"900\" height=\"821\" srcset=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201106.jpg 900w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201106-150x137.jpg 150w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201106-300x274.jpg 300w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201106-768x701.jpg 768w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201106-200x182.jpg 200w\" sizes=\"(max-width: 900px) 100vw, 900px\" \/><\/span><\/p>\n<p style=\"text-align: center;\"><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\">\u56fe3 Y80\u662fRACK1\u7684\u5173\u952e\u7ed3\u5408\u4f4d\u70b9<\/span><\/p>\n<p><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\">A\uff1aEST12\u6676\u4f53\u7ed3\u6784\uff1bB\uff1aEST12-RACK1\u9884\u6d4b\u7ed3\u6784\uff1bC\uff1a\u91cd\u7ec4\u86cb\u767d\u5168\u957f\u3001EST12 C\u7aef\u7f3a\u5931\uff08EST12- D1\uff09\u3001N\u7aef\u7f3a\u5931\uff08EST12- D2\uff09\u3001EST12\u76843\u4e2a\u7a81\u53d8\u4f53\u56fe\u793a\uff1bD-E\uff1a\u91cd\u7ec4\u86cb\u767dpull down\u53caWB\u5206\u6790\uff1bF-I\uff1aLDH\u5206\u6790\u91cd\u7ec4\u86cb\u767d\u4e0d\u540c\u65f6\u95f4\u3001\u4e0d\u540c\u5242\u91cf\u5904\u7406\u8179\u8154\u5de8\u566c\u7ec6\u80de\uff1bJ-K\uff1aLDH\u5206\u6790M. smeg\u548cM. smeg-est12\u3001EST12-D1\u3001EST12-D2\u3001EST12-E55A\u3001EST12-F76A\u3001EST12-Y80A\u611f\u67d3BMDMs\u3002<\/span><\/p>\n<p>&nbsp;<\/p>\n<section><\/section>\n<section><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\"><strong>4\u3001EST12-RACK1\u901a\u8fc7NLRP3\u548cGSDMD\u5f15\u8d77\u5de8\u566c\u7ec6\u80de\u7126\u4ea1\u548cIL-1\u5206\u6ccc<\/strong><\/span><\/section>\n<p><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\">\u708e\u75c7\u5c0f\u4f53\u7684\u6fc0\u6d3b\u662f\u5bf9\u6297\u7ec6\u83cc\u611f\u67d3\u7684\u5173\u952e\u9632\u5fa1\u673a\u5236\uff0c\u7ec6\u83cc\u611f\u67d3\u53ef\u8bf1\u53d1\u5148\u5929\u6027\u514d\u75ab\u53cd\u5e94\uff0c\u5982caspase-1\u6fc0\u6d3b\u548c\u708e\u75c7\u7ec6\u80de\u6b7b\u4ea1\u3002mRNA\u82af\u7247\u5206\u6790EST12\u5904\u7406RAW264.7\u7ec6\u80de\uff0c\u7ed3\u679c\u663e\u793aNLRP3\u3001IL-1\u03b2\u3001IL-6\u548cTNF-\u03b1\u8868\u8fbe\u4e0a\u8c03\u30028\u4e2aPYRIN\u7ed3\u6784\u57df\u86cb\u767d\uff08NLRP1b\u3001NLRP2\u3001NLRP3\u3001NLRP5\u3001NLRP6\u3001NLRP9\u3001NLRP12\u3001PYRIN\uff09\u901a\u8fc7EST12\u5904\u7406\u540e\uff0cNLRP3\u7684mRNA\u8868\u8fbe\u5728\u5de8\u566c\u7ec6\u80de\u663e\u8457\u6539\u53d8\uff08\u56fe4A\uff09\u3002\u5728EST12\u523a\u6fc06\u5c0f\u65f6\u540e\uff0c\u514d\u75ab\u5370\u8ff9\u5206\u6790NLRP3\u86cb\u767d\u8868\u8fbe\u4e0a\u5347\u8fbe\u5230\u6700\u9ad8\uff08\u56fe4B\uff09\u3002\u5171\u805a\u7126\u663e\u5fae\u955c\u5206\u6790\u663e\u793a\uff0cNLRP3\u8868\u8fbe\u5728EST12\u523a\u6fc0\u540e6\u5c0f\u65f6\u6700\u9ad8\uff08\u56fe4C\uff09\u3002EST12\u5904\u7406\u540e\uff0cRACK1\u2212\/\u2212\u4e2dNLRP3\u8868\u8fbe\u660e\u663e\u53d7\u635f\uff08\u56fe4D\uff09\u3002<\/span><\/p>\n<p><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\"><strong>\u7ed3\u679c\u5c0f\u7ed3\uff1aEST12\u5bfc\u81f4NLRP3\u7684\u8868\u8fbe\u4f9d\u8d56\u4e8eRACK1\u3002<\/strong><\/span><\/p>\n<p>&nbsp;<\/p>\n<p><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-42680\" src=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201107.jpg\" alt=\"\" width=\"900\" height=\"951\" srcset=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201107.jpg 900w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201107-142x150.jpg 142w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201107-284x300.jpg 284w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201107-768x812.jpg 768w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201107-200x211.jpg 200w\" sizes=\"(max-width: 900px) 100vw, 900px\" \/><\/span><\/p>\n<p style=\"text-align: center;\"><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\">\u56fe4 EST12\u901a\u8fc7RACK1\u8bf1\u5bfcNLRP3\u708e\u75c7\u5c0f\u4f53\u7684\u6fc0\u6d3b<\/span><\/p>\n<p><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\">\u00a0A\uff1aqPCR\u5206\u6790EST12\u86cb\u767d\u5904\u7406\u7684\u5c0f\u9f20\u8179\u8154\u5de8\u566c\u7ec6\u80de8\u4e2aPYRIN\u7ed3\u6784\u57df\u86cb\u767d\u7684mRNA\u8868\u8fbe\u6c34\u5e73\uff1bB-C\uff1aWB\u548c\u5171\u805a\u7126\u663e\u5fae\u955c\u68c0\u6d4bEST12\u86cb\u767d\u523a\u6fc0\u7684RAW264.7\u7ec6\u80de\uff1bD\uff1aqPCR\u5206\u6790EST12\u5904\u7406WT\u548cRACK1\u2212\/\u2212\u8179\u8154\u5de8\u566c\u7ec6\u80de\u7684NLRP3 mRNA\u8868\u8fbe\u6c34\u5e73\uff1bE\uff1aIP\u548cWB\u5206\u6790\u8d28\u7c92\u8f6c\u67d3\u540eEST12\u523a\u6fc0\u7684RAW264.7\u7ec6\u80de\uff1bF\uff1aWB\u5206\u6790\u88c2\u89e3\u7684BMDMs\u7ec6\u80de\u7684pull down\u86cb\u767d\uff1bG-J\uff1aIP\u548cWB\u5206\u6790EST12\u5904\u7406\u7684BMDMs\u7ec6\u80de\uff1bK\uff1a\u5171\u805a\u7126\u663e\u5fae\u955c\u5206\u6790EST12\u5904\u7406\u540e\u7684WT\u548cRACK1\u2212\/\u2212\u8179\u8154\u5de8\u566c\u7ec6\u80de\uff1bL\uff1aIP\u548cIB\u5206\u6790EST12\u5904\u7406\u6216\u672a\u9884\u5904\u7406\u7684\u8179\u8154\u5de8\u566c\u7ec6\u80de\u3002<\/span><\/p>\n<p>&nbsp;<\/p>\n<p><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\">\u5c06\u7f16\u7801WT\u6cdb\u7d20\uff08H-Ub\uff09\u3001\u7a81\u53d8H-Ub\uff08K48\uff09\u548cH-Ub\uff08K63\uff09\u8d28\u7c92\u8f6c\u67d3\u81f3RAW264.7\u7ec6\u80de\uff0c\u901a\u8fc7IP\u548cWB\u5b9e\u9a8c\uff0c\u63a8\u6d4bEST12\u4ecb\u5bfc\u4e86K48\u8fde\u63a5\u7684NLRP3\u53bb\u6cdb\u7d20\u5316\uff08\u56fe4E\uff09\u3002WB\u5206\u6790EST12\u5904\u7406\u7684RAW 264.7\u7ec6\u80de\u88c2\u89e3\u6db2pull down\u86cb\u767d\uff0c\u8fdb\u4e00\u6b65\u9a8c\u8bc1EST12\u548c\u53bb\u6cdb\u7d20\u9176UCHL5\u7684\u76f8\u4e92\u4f5c\u7528\uff08\u56fe4F\uff09\u3002\u901a\u8fc7\u514d\u75ab\u5171\u6c89\u6dc0\u5206\u6790\uff0c\u53d1\u73b0\u5728EST12\u5904\u7406\u540e\uff0cNLRP3\u548cRACK1\u5747\u76f4\u63a5\u4e0eUCHL5\u76f8\u4e92\u4f5c\u7528\uff08\u56fe4,G-I\uff09\uff0c\u800cEST12-Y80A\u5904\u7406\u4e0d\u80fd\u4fc3\u8fdbRACK1\u4e0eUCHL5\u3001NLRP3\u7684\u76f8\u4e92\u4f5c\u7528\uff08\u56fe4,H\u548cI\uff09\u3002UCHL5\u6572\u4f4e\u682a\u5728EST12\u523a\u6fc0\u540e\uff0cNLRP3\u7684K48\u53bb\u6cdb\u7d20\u5316\u660e\u663e\u964d\u4f4e\uff08\u56fe4J\uff09\uff0c\u7ed3\u679c\u8868\u660eEST12\u523a\u6fc0\u540e\u901a\u8fc7UCHL5\u4ecb\u5bfcNLRP3 K48\u8fde\u63a5\u7684\u53bb\u6cdb\u7d20\u5316\u3002\u514d\u75ab\u8367\u5149\u5206\u6790\u53d1\u73b0EST12\u5b58\u5728\u65f6ASC\u6591\u70b9\u7684\u5f62\u6210\uff0c\u4f46\u5728EST12\u5904\u7406\u7684RACK1\u2212\/\u2212\u8179\u8154\u5de8\u566c\u7ec6\u80de\u4e2d\u5374\u6ca1\u6709\u53d1\u73b0\uff08\u56fe4K\uff09\u3002IP\u548cWB\u5b9e\u9a8c\u53d1\u73b0NLRP3\u53ef\u4ee5\u540c\u65f6\u6c89\u6dc0ASC\u548cpro-caspase-1\uff0cASC\u4e5f\u53ef\u4ee5\u6c89\u6dc0NLRP3\u548cpro-caspase-1\uff08\u56fe4L\uff09\u3002<\/span><\/p>\n<p><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\"><strong>\u7ed3\u679c\u5c0f\u7ed3\uff1a\u5728EST12\u5904\u7406\u540e\uff0cNLRP3\u3001ASC\u548cpro-caspase-1\u53ef\u4ee5\u7ec4\u88c5\u6210NLRP3\u708e\u6027\u5c0f\u4f53\u3002<\/strong><\/span><\/p>\n<p>&nbsp;<\/p>\n<p><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-42681\" src=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201108.jpg\" alt=\"\" width=\"900\" height=\"879\" srcset=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201108.jpg 900w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201108-150x147.jpg 150w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201108-300x293.jpg 300w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201108-768x750.jpg 768w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201108-200x195.jpg 200w\" sizes=\"(max-width: 900px) 100vw, 900px\" \/><\/span><\/p>\n<p style=\"text-align: center;\"><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\">\u56fe5 EST12-RACK1\u901a\u8fc7NLRP3\u548cGSDMD\u5f15\u8d77\u5de8\u566c\u7ec6\u80de\u7126\u4ea1\u548cIL-1\u5206\u6ccc<\/span><\/p>\n<p><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\">A-C\uff1aLDH\u3001ELISA\u5206\u6790EST12\u5904\u7406\u7684WT\u3001NLRP3-\/-\u548cGSDMD-\/-\u8179\u8154\u5de8\u566c\u7ec6\u80de\u6bd2\u6027\u5206\u6790\u53caIL-1\u03b2\u5206\u6ccc\uff1bD\uff1aWB\u68c0\u6d4bEST12\u5904\u7406\u7684WT\u3001NLRP3-\/-\u8179\u8154\u5de8\u566cGSDMD\u548ccaspase-1\u7684\u6d3b\u6027\uff1bE\uff1aWB\u68c0\u6d4bEST12\u548cEST12-Y80A\u5904\u7406\u7684\u8179\u8154\u5de8\u566c\u7ec6\u80deGSDMD\u7684\u6d3b\u6027\uff1bF-H\uff1aLDH\u3001ELISA\u3001WB\u68c0\u6d4bEST12\u5904\u7406WT\u548ccaspase-1\/11\u2212\/\u2212\u8179\u8154\u5de8\u566c\u7ec6\u80de\u6bd2\u6027\u5206\u6790\u3001IL-1\u03b2\u5206\u6ccc\u53caGSDMD\u7684\u6d3b\u6027\uff1bI\u3001J\uff1aLDH\u3001ELISA\u5206\u6790H37Rv\u3001H37Rv\u0394EST12\u53caBCG\u3001BCG-EST12\u611f\u67d3WT\u3001NLRP3\u2212\/\u2212\u3001GSDMD\u2212\/\u2212\u548ccaspase-1\/11\u2212\/\u2212BMDMs\u7ec6\u80de6\u5c0f\u65f6\u540e\u7ec6\u80de\u6bd2\u6027\u548cIL-1\u03b2\u5206\u6ccc\u3002<\/span><\/p>\n<p>&nbsp;<\/p>\n<p><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\">ST12\u5904\u7406GSDMD\u2212\/\u2212\u6216NLRP3\u2212\/\u2212\u5c0f\u9f20\u7684\u8179\u8154\u5de8\u566c\u7ec6\u80de\uff0c\u4e0eWT\u5c0f\u9f20\u8179\u8154\u5de8\u566c\u7ec6\u80de\u76f8\u6bd4\uff0c\u7ec6\u80de\u6bd2\u6027\u76f8\u6bd4\u660e\u663e\u4e0b\u964d\uff08\u56fe5A\u3001B\uff09\uff1b\u4e0a\u6e05\u4e2d\u672a\u68c0\u6d4b\u5230IL-1\u03b2\uff08\u56fe5C\uff09\uff1b\u68c0\u6d4bEST12\u5bf9NLRP3\u2212\/\u2212\u8179\u8154\u5de8\u566c\u7ec6\u80decaspase-1\u548cGSDMD\u88c2\u89e3\u7684\u5f71\u54cd\uff0c\u53d1\u73b0\u4e24\u8005\u5747\u672a\u663e\u793a\u6d3b\u5316\u4f5c\u7528\uff08\u56fe5D\uff09\u3002EST12-Y80A\u5904\u7406WT\u5de8\u566c\u7ec6\u80de\u672a\u88c2\u89e3GSDMD\uff08\u56fe5E\uff09\u3002IL-1\u03b2\u5206\u6ccc\u660e\u663e\u964d\u4f4e\uff08\u56fe5F\u3001G\uff09\uff0c\u672a\u89c2\u5bdf\u5230GSDMD\u7684\u88c2\u89e3\uff08\u56fe5H\uff09\uff0c\u7ed3\u679c\u8868\u660eEST12\u901a\u8fc7caspase-1\u5f15\u53d1\u5de8\u566c\u7ec6\u80deGSDMD\u4ecb\u5bfc\u7684\u7126\u89e3\u548cIL-1\u5206\u6ccc\u3002H37Rv\u4e0eH37Rv\u0394EST12\u611f\u67d3NLRP3\u2212\/\u2212\u3001GSDMD\u2212\/\u2212\u3001caspase-1\/11\u2212\/\u2212BMDMs\u7ec6\u80de\u6bd2\u6027\u3001IL-1\u03b2\u5206\u6ccc\u5e76\u65e0\u5dee\u5f02\uff0c\u9664WT BMDMs\uff08\u56fe5I\uff09\u3002BCG-EST12\u548cBCG\u611f\u67d3\u4e5f\u662f\u7c7b\u4f3c\u60c5\u51b5\u3002<\/span><\/p>\n<p><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\"><strong>\u7ed3\u679c\u5c0f\u7ed3\uff1aEST12-Y80\u901a\u8fc7RACK1\u3001NLRP3\u3001GSDMD\u3001caspase-1\u89e6\u53d1\u5de8\u566c\u7ec6\u80de\u7126\u4ea1\u548cIL-1\u5206\u6ccc\u3002<\/strong><\/span><\/p>\n<p>&nbsp;<\/p>\n<section><\/section>\n<section><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\"><strong>5\u3001EST12\u6fc0\u6d3b\u5bbf\u4e3b\u514d\u75ab\u53cd\u5e94\uff0c\u589e\u5f3a\u5206\u679d\u6746\u83cc\u6e05\u9664<\/strong><\/span><\/section>\n<p><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\">\u5728\u611f\u67d3\u8fc7\u7a0b\u4e2d\uff0c\u88ab\u611f\u67d3\u5de8\u566c\u7ec6\u80de\u7684\u7126\u4ea1\u4f5c\u7528\u662f\u6fc0\u6d3b\u5bbf\u4e3b\u514d\u75ab\u7cfb\u7edf\u7684\u5fc5\u8981\u6761\u4ef6\u3002\u6784\u5efa<em>M.tb<\/em>\u00a0H37Rv\u548cH37Rv\u0394EST12\u5c0f\u9f20\u611f\u67d3\u6a21\u578b\uff0c\u611f\u67d330\u5929\u540e\uff0c\u8fdb\u884c\u80ba\u90e8\u83cc\u843d\u8ba1\u6570\u3001\u8840\u6e05\u4e2dIL-1\u03b2\u68c0\u6d4b\u53ca\u80ba\u90e8\u5207\u7247\u6297\u9178\u67d3\u8272\uff08\u56fe6A-C\uff09\u3002\u80ba\u90e8\u5207\u7247HE\u67d3\u8272\u75c5\u7406\u5b66\u5206\u6790\u4e2d\uff0cH37Rv\u0394EST12\u611f\u67d3\u7ec4\u76f8\u8f83\u4e8eH37Rv\u7ec4\uff0c\u6dcb\u5df4\u7ec6\u80de\u6d78\u6da6\u589e\u591a\u3001\u80ba\u6ce1\u95f4\u9694\u53d8\u5c0f\uff08\u56fe6D\uff09\u3002BCG-EST12\u76f8\u8f83\u4e8eBCG\u611f\u67d3\u7684\u5c0f\u9f20\u80ba\u90e8\u8f7d\u83cc\u91cf\u964d\u4f4e\uff08\u56fe6E\uff09\u3001IL-1\u03b2\u5206\u6ccc\u589e\u52a0\uff08\u56fe6F\uff09\uff0c\u75c5\u7406\u5206\u6790\u663e\u793a\u611f\u67d3BCG\u7ec4\u80ba\u6ce1\u7ec4\u7ec7\u635f\u4f24\u8f83BCG-EST12\u7ec4\u4e25\u91cd\uff08\u56fe6G\uff09\u3002\u5bf9\u4e0d\u540c\u7ec6\u83cc\u611f\u67d3\u540e\u7684WT BMDMs\u8fdb\u884c\u83cc\u843d\u8ba1\u6570\uff0c\u5728WT BMDMs\u4e2d\uff0cBCG-EST12\u611f\u67d3\u4e0eBCG\u7ec4\u76f8\u6bd4\uff0c\u57283\u548c6 hpi\u65f6CFUs\u66f4\u5c11\uff08\u56fe6H\uff09\u3002BCG-EST12\u548cBCG\u611f\u67d3RACK1\u2212\/\u2212,NLRP3\u2212\/\u2212,GSDMD\u2212\/\u2212\u548ccaspase-1\/11\u2212\u2212\/BMDMs\u540e\uff0c\u83cc\u843d\u8ba1\u6570\u6ca1\u6709\u4e0d\u540c\uff08\u56fe6I\u3001J\uff09\u3002<\/span><\/p>\n<p><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\"><strong>\u7ed3\u679c\u5c0f\u7ed3\uff1aEST12\u589e\u52a0\u4e86\u5de8\u566c\u7ec6\u80de\u548c\u5c0f\u9f20\u5bf9M. tb\u611f\u67d3\u62b5\u6297\u529b,\u5b83\u901a\u8fc7RACK1-\u3001NLRP3\u3001GSDMD\u548ccaspase-1\/11-\u4ecb\u5bfc\u7684\u708e\u75c7\u7126\u4ea1\u5728M. tb\u8bf1\u5bfc\u514d\u75ab\u4e2d\u626e\u6f14\u5173\u952e\u7684\u89d2\u8272\u3002<\/strong><\/span><\/p>\n<p>&nbsp;<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-42686\" src=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201112.jpg\" alt=\"\" width=\"523\" height=\"477\" srcset=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201112.jpg 523w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201112-150x137.jpg 150w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201112-300x274.jpg 300w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2020\/11\/20201112-200x182.jpg 200w\" sizes=\"(max-width: 523px) 100vw, 523px\" \/><\/p>\n<p style=\"text-align: center;\"><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\">\u56fe6 EST12\u6fc0\u6d3b\u5bbf\u4e3b\u514d\u75ab\u53cd\u5e94\u589e\u52a0\u5206\u679d\u6746\u83cc\u6e05\u9664<\/span><\/p>\n<p><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\">A\uff1a\u5c0f\u9f20\u611f\u67d3H37Rv\u3001H37Rv\u0394EST12\u83cc\u682a30\u5929\u540e\uff0c\u80ba\u90e8\u83cc\u843d\u8ba1\u6570\uff1bB\uff1aELISA\u68c0\u6d4b\u8840\u6e05\u4e2dIL-1\u03b2\uff1bC\uff1aH37Rv\u3001H37Rv\u0394EST12\u611f\u67d3\u5c0f\u9f20\u80ba\u90e8\u5207\u7247\u6297\u9178\u67d3\u8272\uff1bD\uff1aH37Rv\u3001H37Rv\u0394EST12\u611f\u67d3\u5c0f\u9f20\u80ba\u90e8\u5207\u7247HE\u67d3\u8272\uff1bE\uff1a\u5c0f\u9f20\u611f\u67d3BCG\u3001BCG-EST12\u80ba\u90e8\u83cc\u843d\u8ba1\u6570\uff1bF\uff1aELISA\u68c0\u6d4b\u8840\u6e05\u4e2dIL-1\u03b2\uff1bG\uff1aBCG\u3001BCG-EST12\u611f\u67d3\u5c0f\u9f20\u80ba\u90e8\u5207\u7247HE\u67d3\u8272\uff1bH-J\uff1aWT\uff08H\uff09\u548cRACK1\u2212\/\u2212\uff08J\uff09 BMDMs\u611f\u67d3BCG\u548cBCG-EST12\u6216WT\uff0cNLRP3\u2212\/\u2212\uff0cGSDMD\u2212\/\u2212\uff0ccaspase-1\/11\u2212\/\u2212BMDMs\uff08I\uff09\u611f\u67d36\u5c0f\u65f6\u83cc\u843d\u8ba1\u6570\u3002<\/span><\/p>\n<p>&nbsp;<\/p>\n<section><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\"><strong>\u7ed3\u8bba\uff1a<\/strong><\/span><\/section>\n<p><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\">EST12\u901a\u8fc7\u4e0e\u5de8\u566c\u7ec6\u80deRACK1\u76f8\u4e92\u4f5c\u7528\u800c\u5f15\u8d77\u7ec6\u80de\u7126\u4ea1\u3002RACK1\u4f5c\u4e3a\u68c0\u6d4b<em>M. tb<\/em>\u00a0EST12\u7684\u4e3b\u8981\u5bbf\u4e3b\u56e0\u5b50\uff0c\u5e76\u62db\u52df\u53bb\u6cdb\u7d20\u9176UCHL5\u4fc3\u8fdbNLRP3\u53bb\u6cdb\u7d20\u5316\uff0c\u8fdb\u800c\u6fc0\u6d3b\u5de8\u566c\u7ec6\u80deNLRP3\u708e\u6027\u5c0f\u4f53-caspase-1-GSDMD\u7ec6\u80de\u7126\u4ea1-IL-1\u03b2\u5206\u5b50\u514d\u75ab\u901a\u8def\u3002\u8be5\u7814\u7a76\u9610\u91ca\u4e86\u5de8\u566c\u7ec6\u80de\u4e0eEST12\u4e4b\u95f4\u7684\u76f8\u4e92\u4f5c\u7528\uff0c\u5e76\u8bc1\u5b9e\u4e86EST12-RACK1\u8bf1\u5bfc\u7684\u7ec6\u80de\u7126\u4ea1\u5728<em>M. tb<\/em>\u8bf1\u5bfc\u514d\u75ab\u4e2d\u7684\u5173\u952e\u4f5c\u7528\u3002<\/span><\/p>\n<p>&nbsp;<\/p>\n<p><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\"><strong>\u6587\u732e\u6765\u6e90\uff1a<\/strong><\/span><\/p>\n<p><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\">https:\/\/advances.sciencemag.org\/content\/6\/43\/eaba4733<\/span><\/p>\n<p>&nbsp;<\/p>\n<section><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\">\u6676\u8bfa\u751f\u7269\u662f\u4e00\u5bb6\u4e13\u6ce8\u4e8e\u7ed3\u6838\u5206\u679d\u6746\u83cc\u76f8\u5173\u7814\u7a76\u7684\u516c\u53f8\uff0c\u662f\u76ee\u524d\u56fd\u5185\u552f\u4e00\u4e00\u5bb6\u63d0\u4f9b\u7ed3\u6838\u5206\u679d\u6746\u83cc\u7a81\u53d8\u4f53\u6784\u5efa\u6280\u672f\u670d\u52a1\u548c\u4ea7\u54c1\u7684\u516c\u53f8\uff0c\u5df2\u591a\u6b21\u52a9\u529b\u79d1\u7814\u56e2\u961f\u653b\u5173\u91cd\u8981\u7ed3\u6838\u57fa\u7840\u7814\u7a76\u5de5\u4f5c\uff01<\/span><\/section>\n<section><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\">\u76ee\u524d\u6676\u8bfa\u5df2\u5b8c\u6210\u5c06\u8fd1<strong>1800<\/strong>\u4e2a\u7ed3\u6838\u5206\u679d\u6746\u83cc\u57fa\u56e0\u6572\u9664\u83cc\u682a\uff0c\u8fd8\u5728\u6301\u7eed\u66f4\u65b0\u4e2d\uff01<\/span><\/section>\n<section><\/section>\n<section><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\">\u6572\u9664\u83cc\u682a\u5217\u8868\uff1a<\/span><\/section>\n<section><a href=\"https:\/\/www.gene-optimal.com\/latest-mycobacterium-tuberculosis-knockout-strain\/\">https:\/\/www.gene-optimal.com\/latest-mycobacterium-tuberculosis-knockout-strain\/<\/a><\/section>\n<section><\/section>\n<section><\/section>\n<section><\/section>\n<section>\u8054\u7cfb\u6211\u4eec<\/p>\n<section>\u4e0a\u6d77\u6676\u8bfa\u751f\u7269\u79d1\u6280\u6709\u9650\u516c\u53f8<\/section>\n<section>\u7535\u8bdd\uff1a0757-29328131-616<\/section>\n<section>\u624b\u673a\/\u5fae\u4fe1\uff1a13392273325<\/section>\n<section>Q Q\uff1a2775427010<\/section>\n<section>\u4e1a\u52a1\uff1ainfo@gene-optimal.com<\/section>\n<section>\u62db\u8058\uff1ahr@gene-optimal.com<\/section>\n<section>\u5b98\u7f51\uff1awww.gene-optimal.com<\/section>\n<section><span style=\"background-color: #ebf5ff; color: #3366ff; font-size: 10pt;\">\u7248\u6743\u58f0\u660e\uff1a\u672c\u6587\u7cfb\u6676\u8bfa\u751f\u7269\u7f16\u8bd1\u6574\u7406\uff0c\u672a\u7ecf\u6676\u8bfa\u751f\u7269\u6388\u6743\uff0c\u4e0d\u5f97\u8f6c\u8f7d\u548c\u4f7f\u7528\uff0c\u5982\u9700\u83b7\u53d6\u6388\u6743\uff0c\u8bf7\u8054\u7cfb\u6211\u4eec\u3002<\/span><\/section>\n<\/section>\n<section><\/section>\n<section><\/section>\n<section><\/section>\n","protected":false},"excerpt":{"rendered":"<p>2020\u5e7410\u670821\u65e5\uff0c\u6b66\u6c49\u5927\u5b66\u7ae0\u6653\u8054\u3001\u6bb7\u96f7\u8001\u5e08\u4f5c&#8230;<\/p>\n","protected":false},"author":1,"featured_media":42687,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[4],"tags":[],"_links":{"self":[{"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/posts\/42673"}],"collection":[{"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/comments?post=42673"}],"version-history":[{"count":1,"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/posts\/42673\/revisions"}],"predecessor-version":[{"id":45234,"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/posts\/42673\/revisions\/45234"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/media\/42687"}],"wp:attachment":[{"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/media?parent=42673"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/categories?post=42673"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/tags?post=42673"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}