{"id":41679,"date":"2020-04-22T16:39:36","date_gmt":"2020-04-22T08:39:36","guid":{"rendered":"https:\/\/www.gene-optimal.com\/?p=41679"},"modified":"2020-04-27T11:11:07","modified_gmt":"2020-04-27T03:11:07","slug":"mtb-transcriptomics","status":"publish","type":"post","link":"https:\/\/www.gene-optimal.com\/mtb-transcriptomics\/","title":{"rendered":"\u8f6c\u5f55\u7ec4\u5b66\u5e94\u7528\u4e8e\u7ed3\u6838\u5206\u679d\u6746\u83cc\u7684\u7814\u7a76"},"content":{"rendered":"

\u8f6c\u5f55\u7ec4\u5b66\u662f\u529f\u80fd\u57fa\u56e0\u7814\u7a76\u7684\u91cd\u8981\u7ec4\u6210\u90e8\u5206\uff0c\u662f\u4e00\u95e8\u5728\u6574\u4f53\u6c34\u5e73\u7814\u7a76\u57fa\u56e0\u529f\u80fd\u3001\u57fa\u56e0\u7ed3\u6784\u3001\u63ed\u793a\u57fa\u56e0\u8f6c\u5f55\u3001\u8f6c\u5f55\u8c03\u63a7\u89c4\u5f8b\u4ee5\u53ca\u75be\u75c5\u53d1\u751f\u8fc7\u7a0b\u4e2d\u7684\u5206\u5b50\u673a\u7406\u7684\u5b66\u79d1\u3002\u968f\u7740\u4e0b\u4e00\u4ee3\u6d4b\u5e8f\u6280\u672f\u7684\u5feb\u901f\u53d1\u5c55\u53ca\u5e73\u53f0\u7684\u5e02\u573a\u5316\uff0c\u8f6c\u5f55\u7ec4\u5b66\u6d4b\u5e8f\u6280\u672f\u5df2\u7ecf\u5f7b\u5e95\u6539\u53d8\u4e86\u8f6c\u5f55\u7ec4\u5b66\u7684\u601d\u7ef4\u65b9\u5f0f\u3002\u76ee\u524d\uff0c\u6210\u719f\u7684\u8f6c\u5f55\u7ec4\u5b66\u6d4b\u5e8f\u6280\u672f\u4ee5\u5176\u6574\u4f53\u6027\u3001\u7cfb\u7edf\u6027\u3001\u4e2a\u4f53\u7ec4\u7ec7\u5dee\u5f02\u6027\u3001\u65f6\u95f4\u72ec\u7acb\u6027\u7b49\u4f18\u52bf\uff0c\u66f4\u597d\u5730\u63a8\u52a8\u8f6c\u5f55\u7ec4\u5b66\u7814\u7a76\u3002<\/p>\n

\u5e94\u7528\u8f6c\u5f55\u7ec4\u5b66\u6d4b\u5e8f\u6280\u672f\u9488\u5bf9\u7ed3\u6838\u5206\u679d\u6746\u83cc\u5728\u4f53\u5916\u6216\u4f53\u5185\u4e0d\u540c\u73af\u5883\u4e0b\u5404\u57fa\u56e0\u8868\u8fbe\u6c34\u5e73\u8fdb\u884c\u7814\u7a76\uff0c\u901a\u8fc7\u751f\u7269\u4fe1\u606f\u5b66\u5206\u6790\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u529f\u80fd\uff0c\u8fdb\u884c\u529f\u80fd\u5206\u7c7b\uff0c\u66f4\u6709\u6548\u5730\u7814\u7a76\u5404\u79cd\u56e0\u7d20\u523a\u6fc0\u5bf9\u7ed3\u6838\u5206\u679d\u6746\u83cc\u7684\u5f71\u54cd\u673a\u5236\u3002\u4e0b\u9762\u901a\u8fc7\u5bf9\u51e0\u7bc7\u6587\u732e\u7684\u89e3\u8bfb\uff0c\u5e26\u5927\u5bb6\u4e86\u89e3\u4e00\u4e0b\u7ed3\u6838\u5206\u679d\u6746\u83cc\u8102\u8d28\u76f8\u5173\u7684\u8f6c\u5f55\u7ec4\u5b66\u5206\u6790\u4e0e\u5e94\u7528\u3002<\/p>\n

 <\/p>\n

\u6d41\u7a0b\uff1a\u603bRNA\u63d0\u53d6\u2192\u6837\u54c1\u68c0\u6d4b\u2192\u5efa\u5e93\u2192\u4e0a\u673a\u6d4b\u5e8f\u2192\u751f\u7269\u4fe1\u606f\u5b66\u5206\u6790<\/strong><\/p>\n

 <\/p>\n

\u6587\u732e\u89e3\u8bfb1\uff1a\u901a\u8fc7RNAseq\u5206\u6790\u5bcc\u8102\u6761\u4ef6\u4e0b\u7ed3\u6838\u5206\u679d\u6746\u83cc\u7684\u8f6c\u5f55\u7ec4\u5b66<\/strong><\/span><\/p>\n

\"\"<\/p>\n

 <\/p>\n

\u5b9e\u9a8c\u8bbe\u8ba1<\/strong><\/p>\n

\u6d4b\u5b9a\u7ed3\u6838\u5728\u5bcc\u542b\u8102\u8d28\u6761\u4ef6\u4e0b\u6709\u6c27\u548c\u4f4e\u6c27\u7684\u8f6c\u5f55\u7ec4\u5dee\u5f02\uff0c\u4ee5\u6dfb\u52a0\u8461\u8404\u7cd6\u4e3a\u78b3\u6e90\u4f5c\u4e3a\u5bf9\u7167\uff0c\u901a\u8fc7\u8f6c\u5f55\u7ec4\u6d4b\u5e8f\u6280\u672f\u68c0\u6d4b\u5728\u80c6\u56fa\u9187\u3001\u68d5\u6988\u9178\u3001\u786c\u8102\u9178\u3001\u6cb9\u9178\u6df7\u5408\u7269\u4e3a\u78b3\u6e90\u8bf1\u5bfc\u4e0b\u7ed3\u6838\u5206\u679d\u6746\u83cc\u5728\u751f\u957f\u76843\u4e2a\u4ee3\u8c22\u9636\u6bb5\uff08\u6307\u6570\u671f\u3001\u5e73\u53f0\u671f\u3001NRP1\u671f\uff09\u7684\u8f6c\u5f55\u5dee\u5f02\u3002<\/p>\n\n\n\n\n\t\n\n\t\n\t\n\t
\u5b9e\u9a8c\u7ec4\uff08\u8102\u8d28\u4e3a\u78b3\u6e90\uff09<\/th>\u5bf9\u7167\u7ec4\uff08\u8461\u8404\u7cd6\u4e3a\u78b3\u6e90\uff09<\/th>\n<\/tr>\n<\/thead>\n
\u6709\u6c27\u6307\u6570\u751f\u957f\u671f\uff08LE\uff09<\/td>\u6709\u6c27\u6307\u6570\u751f\u957f\u671f\uff08DE\uff09<\/td>\n<\/tr>\n
\u6709\u6c27\u5e73\u53f0\u671f(LS)<\/td>\u6709\u6c27\u5e73\u53f0\u671f\uff08DS\uff09<\/td>\n<\/tr>\n
\u7f3a\u6c27\u6301\u7559\u671f(LNRP1)<\/td>\u7f3a\u6c27\u6301\u7559\u671f\uff08DNRP1\uff09<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n

\u6ce8\uff1anon-replicating persistence stage 1 of hypoxia (NRP1)\uff0c\u5728\u7f3a\u6c27\u6761\u4ef6\u4e0b\uff0c\u7ed3\u6838\u5206\u679d\u6746\u83cc\u4e0d\u590d\u5236\u6301\u7559\u9636\u6bb5\u3002<\/span><\/p>\n

 <\/p>\n

\u751f\u7269\u4fe1\u606f\u5b66\u5206\u6790<\/strong><\/p>\n

DS\u6837\u672c\u7684\u53cd\u4e49reads\u9ad8\u4e8e\u5176\u4ed6\u6837\u672c\uff08\u56fe1-1A\uff09,\u6df1\u5ea6\u6d4b\u5e8f\u7ed3\u679c\u663e\u793a\u5728DS\u3001LS\u3001LNRP1\u6837\u54c1\u4e2d\u975e\u7f16\u7801RNA\u542b\u91cf\u8f83\u591a\uff08\u56fe1-1B\uff09\u3002\u56fe1-2\u4e3a\u4e3b\u8981\u6210\u5206\u5206\u6790\u548c\u805a\u7c7b\u70ed\u56fe\u3002<\/p>\n

\"\"<\/p>\n

\u56fe1-1 RNAseq\u68c0\u6d4b\u7ed3\u679c<\/span><\/p>\n

 <\/p>\n

\"\"<\/p>\n

\u56fe1-2 \u4e3b\u8981\u6210\u5206\u5206\u6790\u56fe<\/span><\/p>\n

 <\/p>\n

\u8102\u8d28\u6761\u4ef6\u4e0b\u4e0d\u540c\u57fa\u56e0\u8868\u8fbe\u5206\u6790<\/strong><\/p>\n

\u7ed3\u679c\u663e\u793a\u6709368\u4e2a\u57fa\u56e0\u8868\u8fbe\u91cf\u53d8\u5316\uff0c183\u4e2a\u57fa\u56e0\u8868\u8fbe\u91cf\u51cf\u5c11\uff0c185\u4e2a\u57fa\u56e0\u7684\u8868\u8fbe\u91cf\u589e\u52a0\uff08\u56fe1-3A\uff09\u3002\u56fe3B\u4e3a\u4e3b\u8981\u6210\u5206\u5206\u6790\u548c\u805a\u7c7b\u70ed\u56fe\uff0c\u901a\u8fc7\u4e0d\u540c\u78b3\u6e90\uff08\u8102\u8d28\u548c\u8461\u8404\u7cd6\uff09\u53ca\u751f\u7269\u91cd\u590d\u805a\u96c6\u3002\u5dee\u5f02\u57fa\u56e0\u529f\u80fd\u5206\u7c7b\uff08\u56fe1-3C\uff09\uff1a\u5927\u90e8\u5206\u5c5e\u4e8e\u5047\u8bbe\u6027\u86cb\u767d\u3001\u7ec6\u80de\u58c1\u548c\u7ec6\u80de\u8fc7\u7a0b\u3001\u7ec6\u80de\u4ee3\u8c22\u548c\u547c\u5438\u4ee5\u53ca\u9644\u52a0\u5206\u7c7b\uff08IGR\u548csRNA\uff09\u3002\u7ed3\u679c\u8868\u660e\uff0c\u8102\u8d28\u7684\u5b58\u5728\u4fc3\u8fdb\u8102\u7c7b\u4ee3\u8c22\u7c7b\u3001\u4e2d\u95f4\u4ee3\u8c22\u7c7b\u3001\u547c\u5438\u7c7b\u57fa\u56e0\u8868\u8fbe\uff08\u56fe1-3D\uff09\u3002<\/p>\n

\"\"<\/p>\n

\u56fe1-3 \u8102\u8d28\u6761\u4ef6\u4e0b\u7ed3\u6838\u5206\u679d\u6746\u83cc\u5dee\u5f02\u57fa\u56e0\u8868\u8fbe<\/span><\/p>\n

 <\/p>\n

\u8102\u8d28\u6761\u4ef6\u4e0b\u7ed3\u6838\u5206\u679d\u6746\u83cc\u4e0d\u540c\u751f\u957f\u9636\u6bb5\u5404\u57fa\u56e0\u8868\u8fbe\u5206\u6790<\/strong><\/p>\n

\u4ee5\u8461\u8404\u7cd6\u4e3a\u78b3\u6e90\u505a\u5bf9\u7167\uff0c\u8102\u8d28\u4e3a\u78b3\u6e90\u7684\u6761\u4ef6\u4e0b\uff0c\u5728LE\u9636\u6bb5\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u67091241\u4e2a\uff08\u5176\u4e2d557\u4e2a\u4e0b\u8c03\uff0c684\u4e2a\u4e0a\u8c03\uff09\uff1b\u5728LS\u9636\u6bb5\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u67092043\u4e2a\uff08\u5176\u4e2d1196\u4e2a\u4e0b\u8c03\uff0c847\u4e2a\u4e0a\u8c03\uff09\uff1b\u5728LNRP1\u9636\u6bb5\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u6709138\u4e2a\uff08\u5176\u4e2d43\u4e2a\u4e0b\u8c03\uff0c95\u4e2a\u4e0a\u8c03\uff09\uff08\u56fe1-4\uff09\u3002<\/p>\n

\"\"<\/p>\n

\u56fe1-4 \u8102\u8d28\u6761\u4ef6\u4e0b\u4e0d\u540c\u751f\u957f\u9636\u6bb5\u7684\u5dee\u5f02\u57fa\u56e0\u8868\u8fbe<\/span><\/p>\n

 <\/p>\n

\u5982\u56fe1-5\u6240\u793a\uff0c\u8868\u793a\u6bcf\u7c7b\u529f\u80fd\u57fa\u56e0\u8868\u8fbe\u5dee\u5f02\u4e0a\u4e0b\u8c03\u6570\u76ee\uff0c\u5dee\u5f02\u6700\u591a\u7684\u7c7b\u522b\u6709\uff1a\u4fdd\u5b88\u5047\u8bbe\u86cb\u767d\u3001\u7ec6\u80de\u58c1\u548c\u7ec6\u80de\u8fc7\u7a0b\u3001\u4ee3\u8c22\u548c\u547c\u5438\u4ee5\u53ca\u9644\u52a0\u5206\u7c7b\uff08IGR\u548csRNA\uff09\u3002\u56fe5B\u4e3a\u4e0d\u540c\u529f\u80fd\u7c7b\u522b\u5206\u6790\u3002\u901a\u8fc7\u6b64\u5206\u6790\uff0c\u53ef\u5bf9\u6bcf\u4e2a\u751f\u957f\u9636\u6bb5\u7684\u57fa\u56e0\u4e0a\u4e0b\u8c03\u7c7b\u522b\u5206\u7c7b\u3002\u5728LS\u548cLNRP1\u9636\u6bb5\uff0cPE\/PPE\u7684\u8868\u8fbe\u91cf\u660e\u663e\u4e0b\u964d\uff0c\u800c\u76f8\u5173\u8102\u8d28\u3001\u4e2d\u95f4\u4ee3\u8c22\u548c\u547c\u5438\u7684\u57fa\u56e0\u8868\u8fbe\u91cf\u660e\u663e\u4e0a\u8c03\u3002\u5728LS\u9636\u6bb5\uff0c\u4fe1\u606f\u901a\u8def\u53ca\u7ec6\u80de\u58c1\u548c\u7ec6\u80de\u8fc7\u7a0b\u8fd9\u4e24\u7c7b\u8868\u8fbe\u4e0a\u8c03\uff0c\u5728LNRP1\u7f3a\u6c27\u9636\u6bb5\uff0c\u6bd2\u529b\u548c\u8c03\u63a7\u86cb\u767d\u8868\u8fbe\u4e0a\u8c03\u3002\u5728LE\u9636\u6bb5\u53ea\u6709\u4fe1\u606f\u901a\u8def\u76f8\u5173\u7c7b\u522b\u8868\u8fbe\u660e\u663e\u4e0b\u8c03\u3002<\/p>\n

 <\/p>\n

\"\"<\/p>\n

\u56fe1-5 \u8102\u8d28\u6761\u4ef6\u4e0b\u4e0d\u540c\u751f\u957f\u9636\u6bb5\u7684\u5dee\u5f02\u57fa\u56e0\u7684\u529f\u80fd\u5206\u7c7b<\/span><\/p>\n

 <\/p>\n

\u4e3b\u8981\u7684\u6838\u5fc3\u8102\u8d28\u53cd\u5e94<\/strong><\/p>\n

\u56fe1-6\u4e3a\u8102\u8d28\u8bf1\u5bfc\u7684\u57fa\u56e0\u6570\u76ee\uff0c\u5c55\u793a\u4e86\u5728\u8102\u8d28\u6761\u4ef6\u4e0b\u8868\u8fbe\u91cf\u663e\u8457\u589e\u52a0\u57fa\u56e0\u3002\u4e2d\u5fc3\u7684\u91cd\u53e0\u533a\u57df\u4e3a\u5728\u4e09\u4e2a\u9636\u6bb5\u8868\u8fbe\u91cf\u90fd\u660e\u663e\u5347\u9ad8\u7684\u57fa\u56e0\uff0c\u79f0\u4e3a\u6838\u5fc3\u8102\u8d28\u53cd\u5e94\uff0c\u67096\u4e2a\u57fa\u56e0\u5206\u522b\u4e3a\uff1aRv3161c\u3001Rv3160c\u3001Rv0678\u3001Rv1217c\u3001PPE53<\/em>\u548cche1<\/em>\u3002<\/p>\n

\"\"<\/p>\n

\u56fe1-6 \u8102\u8d28\u8bf1\u5bfc\u7684\u6838\u5fc3\u57fa\u56e0<\/span><\/p>\n

 <\/p>\n

qPCR\u9a8c\u8bc1<\/strong><\/p>\n

\u9009\u62e910\u4e2a\u5728\u8102\u8d28\u6761\u4ef6\u4e0b\u660e\u663e\u4e0a\u8c03\u57fa\u56e0\uff0c\u901a\u8fc7qPCR\u9a8c\u8bc1\u3002\u5176\u4e2d7\u4e2a\u57fa\u56e0\u4e3aRv3160c\u3001Rv3161c\u3001PPE53<\/em>\u3001che1<\/em>\u3001mmpS5<\/em>\u3001hsd4A<\/em>\u548cusfY<\/em>\uff08DGE\u5206\u6790\u5728\u8102\u8d28\u4e09\u4e2a\u9636\u6bb5\u5747\u4e0a\u8c03\u57fa\u56e0\uff09,\u53e6\u59163\u4e2a\u4e3ahrp1<\/em>\uff08LE\u9636\u6bb5\u589e\u52a0\uff09\u3001Rv1639c\uff08LS\u9636\u6bb5\u589e\u52a0\uff09\u3001Rv0560\uff08LNRP1\u9636\u6bb5\u589e\u52a0\uff09\u3002\u6d4b\u5b9a\u8fd910\u4e2a\u57fa\u56e0\u7684\u7edd\u5bf9\u8868\u8fbe\u91cf\uff08\u4ee516s RNA\u4e3a\u5185\u53c2\uff09\uff08\u56fe1-7A\uff09\uff0c\u4ee5\u8461\u8404\u7cd6\u4e3a\u78b3\u6e90\u505a\u5bf9\u7167\u7ec4\uff0c\u8102\u8d28\u6761\u4ef6\u4e0b\u5404\u57fa\u56e0\u8868\u8fbe\u91cf\uff08\u56fe1-7B\uff09\u3002<\/p>\n

 <\/p>\n

\"\"<\/p>\n

\u56fe1-7 qPCR\u9a8c\u8bc1\u8102\u8d28\u6761\u4ef6\u4e0b\u57fa\u56e0\u8868\u8fbe\u5dee\u5f02<\/span><\/p>\n

 <\/p>\n

qPCR\u7ed3\u679c\u4e0e\u4e0a\u8ff0\u8f6c\u5f55\u7ec4\u5b66\u7ed3\u679c\u4e00\u81f4\uff0c\u5176\u4e2dhrp1<\/em>\u3001Rv1639c\u548cRv0560\u5206\u522b\u5728LE\u3001LS\u548cLNRP1\u4e2d\u8868\u8fbe\u6700\u591a\uff0cPPE53\u5728LS\u9636\u6bb5\uff0cche1<\/em>\u5728LE\u9636\u6bb5\u65e0\u7edf\u8ba1\u5b66\u610f\u4e49\u3002<\/p>\n

\u8f6c\u5f55\u7ec4\u5b66\u6570\u636e\u8868\u660e\uff0c\u8102\u8d28\u4f5c\u4e3a\u78b3\u6e90\u53ef\u8bf1\u5bfc\u6838\u5fc3\u8102\u8d28\u53cd\u5e94\u30026\u4e2a\u6838\u5fc3\u8102\u8d28\u53cd\u5e94\u57fa\u56e0\u4e0e\u8010\u836f\u3001\u94c1\u7684\u83b7\u53d6\u53ca\u786b\u51cf\u5c11\u76f8\u5173\uff0c\u4ee3\u8c22\u901a\u8def\u5982\u56fe1-8\u6240\u793a\u3002\u5728\u7ec6\u80de\u58c1\u548c\u547c\u5438\u6291\u5236\u5242\u5b58\u5728\u7684\u60c5\u51b5\u4e0b\uff0c\u4e5f\u53d1\u73b0\u4e86Rv3160c-RV3161c\u548cPPE53<\/em>\u7684\u76f8\u5173\u8fc7\u8868\u8fbe\u3002mmpL5<\/em>–mmpS5<\/em>\u4e0d\u4ec5\u4e0e\u8010\u836f\u76f8\u5173\uff0c\u4e5f\u4e0e\u94c1\u86cb\u767d\u8f6c\u8fd0\u76f8\u5173\u3002\u53e6\u4e00\u4e2a\u4e0e\u94c1\u5229\u7528\u76f8\u5173\u7684\u5e73\u5747\u6838\u5fc3\u8102\u8d28\u53cd\u5e94\u57fa\u56e0\u662fche1<\/em>\uff0c\u5176\u7f16\u7801\u4ea7\u7269\u662f\u94c1\u87af\u5408\u9176\u3002Rv1217c\u88ab\u9884\u6d4b\u4e3aRv1216c\u3001Rv1217c\u3001Rv1218c\u548cRv1219c\u64cd\u7eb5\u5b50\u7684\u4e00\u90e8\u5206\uff0c\u5728\u8102\u8d28\u5b58\u5728\u7684\u60c5\u51b5\u4e0b\uff0c\u5b83\u4eec\u7684\u8868\u8fbe\u91cf\u63d0\u9ad8\u3002\u540c\u6837\uff0c\u8be5\u64cd\u7eb5\u5b50\u5728\u836f\u7269\u5b58\u5728\u65f6\u4e0emmpS5-mmpL5<\/em>\u5171\u540c\u8868\u8fbe\u3002\u540c\u6837\u91cd\u8981\u7684\u8fd8\u6709\u5916\u6392\u6cf5\u7f16\u7801\u57fa\u56e0mmr<\/em>\u548c\u786b\u8fd8\u539f\u64cd\u7eb5\u5b50(Rv2391-Rv2394)\u7684\u8fc7\u8868\u8fbe\uff0c\u5728\u672c\u7814\u7a76\u4e2d\uff0c\u6240\u6709\u8fd9\u4e9b\u57fa\u56e0\u90fd\u4ee5\u67d0\u79cd\u65b9\u5f0f\u8054\u7cfb\u5728\u4e00\u8d77\uff0c\u5e76\u4e14\u5728\u8102\u8d28\u5b58\u5728\u7684\u60c5\u51b5\u4e0b\u663e\u8457\u8fc7\u8868\u8fbe\u3002\u6839\u636e\u672c\u7814\u7a76\u63a8\u6d4b\u7ed3\u6838\u5206\u679d\u6746\u83cc\u5229\u7528\u8102\u8d28\u4e3a\u78b3\u6e90\u5408\u6210\u65b0\u7684\u8102\u8d28\u53ca\u7ec6\u80de\u58c1\u91cd\u6784\u3002\u672c\u7814\u7a76\u4e2d\u8102\u8d28\u4e5f\u8bf1\u5bfc\u4e86\u975e\u7f16\u7801\u57fa\u56e0\u7684\u8fc7\u91cf\u8868\u8fbe\uff0c\u4e5f\u8bc1\u5b9e\u4e86\u4e4b\u524d\u7684\u7814\u7a76\uff0cncRNA\u4e0e\u7ed3\u6838\u5b58\u6d3b\u5bc6\u5207\u76f8\u5173\u3002<\/p>\n

\"\"<\/p>\n

\u56fe1-8 \u6838\u5fc3\u8102\u8d28\u53cd\u5e94\u6fc0\u6d3b\u7684\u4e3b\u8981\u4ee3\u8c22\u901a\u8def<\/span><\/p>\n

 <\/p>\n

\u968f\u7740\u8d8a\u6765\u8d8a\u591a\u7684\u7814\u7a76\u8bc1\u5b9e\u4e86\u8102\u8d28\u5728\u7ed3\u6838\u75c5\u75c5\u7406\u8fc7\u7a0b\u4e2d\u7684\u91cd\u8981\u6027\uff0c\u6838\u5fc3\u8102\u8d28\u53cd\u5e94\u53ef\u80fd\u4e3a\u6211\u4eec\u63d0\u4f9b\u4e00\u4e9b\u7ebf\u7d22\uff0c\u4ee5\u786e\u5b9aMtb\u4ee3\u8c22\u4e2d\u7684\u76f8\u5173\u9014\u5f84\uff0c\u5e76\u627e\u5230\u66f4\u597d\u7684\u6cbb\u7597\u9776\u70b9\uff0c\u7528\u4e8e\u7ed3\u6838\u75c5\u60a3\u8005\u7684\u6297\u751f\u7d20\u548c\u75ab\u82d7\u5f00\u53d1\u4ee5\u53ca\u66f4\u597d\u7684\u6cbb\u7597\u65b9\u6848\u3002<\/p>\n

 <\/p>\n

\u6587\u732e\u89e3\u8bfb2\uff1ammpL3<\/em>\u57fa\u56e0\u6c89\u9ed8\u5bf9\u7ed3\u6838\u5206\u679d\u6746\u83cc\u57fa\u56e0\u8868\u8fbe\u7684\u5f71\u54cd<\/strong><\/span><\/p>\n

\"\"<\/p>\n

 <\/p>\n

\u5b9e\u9a8c\u8bbe\u8ba1<\/strong><\/p>\n

\u56db\u73af\u7d20\u8bf1\u5bfc\u7684\u65b9\u6cd5\u5bf9\u5fc5\u9700\u57fa\u56e0mmpL3<\/em>\u6572\u4f4e\u540e\uff0c\u68c0\u6d4bmmpL3<\/em>\u6572\u4f4e\u83cc\u5728\u80de\u5185\u3001\u80de\u5916\u7684\u751f\u5b58\u80fd\u529b\uff0c\u5bf9mmpL3<\/em>\u6572\u4f4e\u83cc\u682a\u8102\u8d28\u6210\u5206\u5206\u6790\uff0c\u901a\u8fc7RNAseq\u68c0\u6d4b\u8be5\u57fa\u56e0\u8868\u8fbe\u91cf\u5bf9\u7ed3\u6838\u5206\u679d\u6746\u83cc\u57fa\u56e0\u8f6c\u5f55\u6c34\u5e73\u7684\u5f71\u54cd\u3002<\/p>\n

 <\/p>\n

\u6784\u5efa\u56db\u73af\u7d20\u4ecb\u5bfc(tet-off)\u7684mmpL3<\/em>\u57fa\u56e0\u6572\u4f4e\u83cc\u682a<\/strong><\/p>\n

\u5c06\u56db\u73af\u7d20\u8bf1\u5bfc\u7684\u6572\u4f4e\u83cc\u682a\u547d\u540d\u4e3aTB416\uff0cmmpL3<\/em>\u4e3a\u5fc5\u9700\u57fa\u56e0\uff0c\u6dfb\u52a0\u56db\u73af\u7d20\u540e\uff0c\u5bf9mmpL3<\/em>\u57fa\u56e0\u7684\u8868\u8fbe\u5177\u6709\u6291\u5236\u4f5c\u7528\uff0cTB416\u5728500ng\/mL\u6d53\u5ea6\u56db\u73af\u7d20\u7684\u5e73\u677f\u4e0a\u4e0d\u80fd\u751f\u957f\uff0c\u5982\u56fe2-1A\u6240\u793a\u3002\u6db2\u4f53\u57f9\u517b\u65f6\uff0c\u6dfb\u52a0\u56db\u73af\u7d20\u5bf9\u751f\u957f\u5177\u6709\u660e\u663e\u7684\u6291\u5236\u4f5c\u7528\uff0c\u5982\u56fe2-1B\u6240\u793a\u3002<\/p>\n

\"\"<\/p>\n

\u56fe2-1 \u6dfb\u52a0\u56db\u73af\u7d20\u5bf9\u6572\u4f4e\u83cc\u682a\u751f\u957f\u5f71\u54cd
\n<\/span>\u6ce8\uff1a\u25cf\u4e3aATC 500ng\/mL;\u25a0\u4e3aATC 100ng\/mL;\u25b2\u65e0ATC\u6dfb\u52a0\u3002<\/span><\/p>\n

 <\/p>\n

\u6572\u4f4e\u83cc\u682aTB416\u8102\u8d28\u6210\u5206\u5206\u6790<\/strong><\/p>\n

\u5bf9\u6dfb\u52a0ATC\u548c\u4e0d\u6dfb\u52a0ATC\u57f9\u517b96\u5c0f\u65f6\u7684\u6572\u4f4e\u83cc\u682a\u3001\u91ce\u751f\u578b\u83cc\u682a\u7684\u8102\u8d28\u5b9a\u6027\u9274\u522b\uff0cmmpL3<\/em>\u57fa\u56e0\u6572\u4f4e\u540e\u5bfc\u81f4\u7ec6\u80de\u819c\u6210\u5206\u53d8\u5316\uff0cTDM\u7684\u51cf\u5c11\u548cTMM\u7684\u7d2f\u79ef\u8f83\u4e3a\u660e\u663e\uff0c\u5982\u56fe2-2\u6240\u793a\u3002<\/p>\n

\"\"<\/p>\n

\u56fe2-2 mmpL3\u6572\u4f4e\u83cc\u682aTMM\u7684\u7d2f\u79ef<\/span><\/p>\n

 <\/p>\n

TB416\u611f\u67d3\u5de8\u566c\u7ec6\u80de<\/strong><\/p>\n

\u5c06\u83cc\u682aTB416\u4fb5\u67d3THP-1\u5de8\u566c\u7ec6\u80de\u540e\uff0c\u5728\u57f9\u517b\u57fa\u4e2d\u6dfb\u52a0ATC\uff08200ng\/mL\uff09\uff0c\u964d\u4f4emmpL3<\/em>\u7684\u8868\u8fbe\u91cf\uff0c\u8bc4\u4f30mmpL3<\/em>\u57fa\u56e0\u8868\u8fbe\u4e0e\u7ed3\u6838\u5206\u679d\u6746\u83cc\u5728\u7ec6\u80de\u751f\u957f\u4e2d\u91cd\u8981\u6027\u3002\u5982\u56fe2-3\u6240\u793a\uff0c\u6dfb\u52a0ATC\u7684TB416\u80de\u5185\u8f7d\u83cc\u91cf\u4e0e\u91ce\u751f\u83cc\u682a\u4e00\u81f4\uff0c\u6dfb\u52a0ATC\u7684TB416\u5728\u7b2c4\u5929\u83cc\u91cf\u4e0e\u91ce\u751f\u83cc\u6bd4\u8f83\u51cf\u5c11100\u500d\uff0c\u663e\u793ammpL3<\/em>\u57fa\u56e0\u662f\u7ed3\u6838\u5206\u679d\u6746\u83cc\u5728\u80de\u5185\u751f\u957f\u3001\u5b58\u6d3b\u6240\u5fc5\u9700\u7684\u3002<\/p>\n

\"\"<\/p>\n

\u56fe2-3 mmpL3\u57fa\u56e0\u6572\u4f4e\u83cc\u682a\u5728\u5de8\u566c\u7ec6\u80de\u5185\u5b58\u6d3b\u60c5\u51b5<\/span>
\n\u6ce8\uff1a\u25cf\u4e3a\u4eb2\u672c\u83cc\u682aTB38\uff1b\u25a0\u4e3a\u65e0ATC\u6dfb\u52a0\u7684TB416;\u25b2\u57f9\u517b\u57fa\u4e2d\u6dfb\u52a0ATC\uff08200ng\/mL\uff09\u7684TB416\u3002<\/span><\/p>\n

 <\/p>\n

\u8f6c\u5f55\u7ec4\u6d4b\u5e8f<\/strong><\/p>\n

\u7ed3\u6838\u5206\u679d\u6746\u83cc\u6dfb\u52a0\u56db\u73af\u7d20\u540e\u6291\u5236mmpL3<\/em>\u57fa\u56e0\u8868\u8fbe\uff0c\u8fdb\u884c\u8f6c\u5f55\u7ec4\u6d4b\u5e8f\uff0c\u4e86\u89e3\u8be5\u57fa\u56e0\u751f\u7269\u529f\u80fd\u3002ATC\u8bf1\u5bfc\u7684TB416\u5bfc\u81f447\u4e2a\u57fa\u56e0\u8868\u8fbe\u91cf\u4e0a\u8c03\uff0c23\u4e2a\u57fa\u56e0\u8868\u8fbe\u91cf\u4e0b\u8c03\uff083\u500d\u4ee5\u4e0a\uff09\uff0c\u4ece\u4e2d\u9009\u62e9\u4e865\u4e2a\u57fa\u56e0\uff082\u4e2a\u4e0a\u8c03\uff0c3\u4e2a\u4e0b\u8c03\uff09qPCR\u9a8c\u8bc1\u8f6c\u5f55\u6570\u636e\u3002<\/p>\n

 <\/p>\n

\u751f\u7269\u4fe1\u606f\u5b66\u5206\u6790<\/strong><\/p>\n

\u572847\u4e2a\u4e0a\u8c03\u57fa\u56e0\u4e2d\uff0c\u670926\u4e2a\u57fa\u56e0\u662f\u4f4d\u4e8e9\u4e2a\u76f8\u90bb\u57fa\u56e0\u7c07\u4e2d\uff0c\u5e76\u534f\u540c\u8f6c\u5f55\u3002Rv1057\u4e3a\u4e0a\u8c03\u6700\u9ad8\u7684\u57fa\u56e0\uff0811.8\u500d\uff09\uff0c\u8be5\u57fa\u56e0\u7f16\u7801\u4e00\u4e2a\u672a\u77e5\u529f\u80fd\u7684\u03b2\u87ba\u65cb\u7ed3\u6784\u86cb\u767d\uff0c\u636e\u62a5\u9053\u8be5\u57fa\u56e0\u5728\u7ed3\u6838\u5206\u679d\u6746\u83cc\u53d7\u5230\u8868\u9762\u80c1\u8feb\u88ab\u8bf1\u5bfc\uff0c\u5e76\u53d7MprA<\/em>\u3001SigE<\/em>\u57fa\u56e0\u7684\u8c03\u63a7\u3002\u94dc\u8bf1\u5bfcmmpL3<\/em>\u7f3a\u5931\u65f6\u5bfc\u81f4\u4e0a\u8c03\u7684\u57fa\u56e0\u79f0\u4e3acso<\/em>\u64cd\u7eb5\u5b50\uff0c\u7f16\u7801\u94dc\u8fd0\u8f93\u7cfb\u7edf\uff0ccadI<\/em>\u3001arsC<\/em>\uff08\u7f16\u7801\u5047\u5b9a\u91d1\u5c5e\u8f6c\u8fd0\u4f53\uff09\uff0cRv2963\uff08\u7f16\u7801\u5047\u5b9aABC\u901a\u900f\u9176\uff09\uff0cmymT <\/em>(\u7f16\u7801\u91d1\u5c5e\u786b\u86cb\u767d\uff0c\u4fdd\u62a4\u7ec6\u80de\u514d\u53d7\u94dc\u4e2d\u6bd2)\uff0cmmcO<\/em>\uff08\u7f16\u7801\u8010\u94dc\u6240\u5fc5\u9700\u7684\u591a\u94dc\u6c27\u5316\u9176\uff09\uff0clpqS-cysK2-rv0849-rv0850<\/em>\u64cd\u7eb5\u5b50,\u5bf9\u6bd2\u529b\u548c\u94dc\u6297\u6027\u81f3\u5173\u91cd\u8981\u3002\u8fd9\u4e9b\u6570\u636e\u8868\u660e\uff0cMmpL3\u7684\u8868\u8fbe\u91cf\u5f71\u54cd\u7ec6\u80de\u901a\u900f\u6027\uff0c\u6270\u4e71\u91d1\u5c5e\u7a33\u6001\uff0c\u4ece\u800c\u5f71\u54cd\u7ec6\u80de\u8d28\u6c27\u5316\u8fd8\u539f\u7535\u4f4d\u3002<\/p>\n

Rv0516c(\u6e17\u900f\u86cb\u767dA)\u53c2\u4e0e\u6e17\u900f\u4fe1\u53f7\u9014\u5f84\uff08\u5305\u542bpknd<\/em>\u3001sigF<\/em>\uff09\uff0c\u8c03\u8282\u80bd\u805a\u7cd6\u67b6\u6784\uff0c\u63a8\u6d4bmmpL3<\/em>\u8868\u8fbe\u51cf\u5c11\uff0c\u8102\u8d28\u6210\u5206\u6539\u53d8\uff0c\u5bfc\u81f4\u8868\u9762\u901a\u900f\u6027\u6539\u53d8\uff0c\u4f7f\u7ec6\u80de\u53d7\u5230\u6e17\u900f\u538b\u80c1\u8feb\u3002\u4e0e\u6b64\u5047\u8bbe\u4e00\u81f4\uff0cargC<\/em>\u57fa\u56e0\u4e0a\u8c033.9\u500d\uff08\u7cbe\u6c28\u9178\u5408\u6210\u64cd\u7eb5\u5b50\u7684\u7b2c\u4e00\u4e2a\u57fa\u56e0\uff09\uff0c\u7cbe\u6c28\u9178\u662f\u4e00\u79cd\u6e17\u900f\u4fdd\u62a4\u5242\u3002iniBAC<\/em>\u64cd\u7eb5\u5b50\uff0c\u5e38\u89c1\u7684\u7ec6\u80de\u58c1\u5e94\u6fc0\u64cd\u7eb5\u5b50\uff0c\u52a0\u5165EMB\u548cINH\u540e\u7ec6\u80de\u58c1\u5408\u6210\u53d7\u5230\u6291\u5236\u3002<\/p>\n

\u4e0a\u8c03\u57fa\u56e0\u8fd8\u5305\u542b\u51e0\u4e2a\u8f6c\u5f55\u8c03\u63a7\u56e0\u5b50\uff0cwhiB6<\/em>\uff08\u8c03\u8282ESX-1\u548cDosR\u8c03\u8282\u5b50\u5bf9NO\u53cd\u5e94\uff0c\u4ece\u800c\u8c03\u8282\u8089\u82bd\u80bf\u5f62\u6210\u548c\u6bd2\u529b\uff09\u548cwhiB7<\/em>\uff08\u8c03\u8282\u51e0\u79cd\u8010\u836f\u57fa\u56e0\uff09\u3002\u63a8\u6d4bMmpL3\u7684\u8017\u7aed\u5f71\u54cd\u7ed3\u6838\u5206\u679d\u6746\u83cc\u6bd2\u529b\u53ca\u836f\u7269\u654f\u611f\u6027\u3002<\/p>\n

\u5728\u88ab\u6291\u52363\u500d\u4ee5\u4e0a\u7684\u57fa\u56e0\u4e2d\u6700\u591a\u7684\u529f\u80fd\u7c7b\u522b\u662f\u8102\u8d28\u4ee3\u8c22\u3002\u572823\u4e2a\u4e0b\u8c03\u57fa\u56e0\u4e2d15\u4e2a\u57fa\u56e0\u57286\u4e2a\u57fa\u56e0\u7c07\u4e0a\u5171\u8f6c\u5f55\uff0c\u6beb\u65e0\u7591\u95ee\uff0c\u4e0b\u8c03\u6700\u591a\u7684\u4e3aMmpL3\uff0c\u540c\u65f6\u7f16\u7801\u5206\u679d\u83cc\u9178\u3001TDM\u751f\u7269\u5408\u6210\u76f8\u5173\u51e0\u4e2a\u57fa\u56e0\u4e0b\u8c03\uff08\u5982acpM-kasA-kasB<\/em>\u3001fbpB<\/em>\uff09\uff0c\u63a8\u6d4bTMM\u7684\u7d2f\u79ef\uff0c\u5b58\u5728\u53cd\u9988\u673a\u5236\u4e0b\u8c03\u8fd9\u4e00\u751f\u7269\u5408\u6210\u9014\u5f84\u3002\u5176\u5b83\u88ab\u6291\u5236\u57fa\u56e0\u53c2\u4e0e\u80fd\u91cf\u751f\u4ea7\u5982sdaA<\/em>\u53c2\u4e0e\u4e1d\u6c28\u9178\u7cd6\u5f02\u751f\uff0catpB<\/em>\u7f16\u7801ATP\u5408\u6210\u9176\uff0cnuoB\u3001D\u3001H<\/em>\u7f16\u7801NADH\u8131\u6c22\u9176\u7684\u4e0d\u540c\u4e9a\u57fa\uff0cmce1<\/em>\u64cd\u7eb5\u5b50\uff0c\u63a8\u6d4b\u5b83\u7f16\u7801\u4e86\u4e00\u4e2a\u8102\u8d28\u518d\u6444\u53d6\u7cfb\u7edf\uff0c\u4f7f\u83cc\u9178\u80fd\u591f\u5faa\u73af\u5229\u7528\uff0c\u8fd9\u8868\u660e\u7ec6\u80de\u7684\u4ee3\u8c22\u6d3b\u52a8\u51cf\u5c11\u4e86\u3002<\/p>\n

\u5bf9\u4ee5\u4e0a\u6570\u636e\u7684\u5206\u6790\uff0c\u8868\u660eMmpL3\u7684\u7f3a\u5931\u5bfc\u81f4\u4e86\u8868\u9762\u901a\u900f\u6027\u7684\u6539\u53d8\uff0c\u53ef\u80fd\u662f\u7531\u4e8e\u4e0d\u80fd\u6b63\u786e\u5730\u6784\u5efa\u7ec6\u80de\u58c1\uff0c\u5bfc\u81f4\u6e17\u900f\u80c1\u8feb\u548c\u91d1\u5c5e\u7a33\u6001\u7684\u7834\u574f\uff0c\u8fd9\u4e9b\u662fMmpL3\u7f3a\u5931\u5f71\u54cd\u7ec6\u80de\u6d3b\u529b\u7684\u4e3b\u8981\u673a\u5236\u3002<\/p>\n

 <\/p>\n

\u8f6c\u5f55\u7ec4\u5b66\u5e38\u7528\u5e94\u7528\u5957\u8def\u56fe\uff1a<\/strong><\/span><\/p>\n

\"\"<\/p>\n

\u9488\u5bf9\u836f\u7269\u4f5c\u7528\u673a\u5236\u3001\u8010\u836f\u673a\u5236\u7684\u7814\u7a76\uff0c\u627e\u5230\u6838\u5fc3\u9776\u70b9\u57fa\u56e0\u540e\uff0c\u8fdb\u4e00\u6b65\u6df1\u5165\u7684\u540e\u7eed\u7814\u7a76\u53ef\u4ee5\u8003\u8651\uff0c\u901a\u8fc7\u5bf9\u8be5\u57fa\u56e0\u7684\u6572\u9664\u6216\u8fc7\u8868\u8fbe\u83cc\u682a\u6784\u5efa\uff0c\u9a8c\u8bc1\u8be5\u83cc\u682a\u5bf9\u836f\u7269\u7684MIC\u662f\u5426\u53d1\u751f\u53d8\u5316\uff0c\u4ece\u800c\u8fdb\u4e00\u6b65\u786e\u5b9a\u8be5\u9776\u70b9\u3002<\/strong><\/span><\/p>\n

 <\/p>\n

\u53c2\u8003\u6587\u732e\uff1a<\/span><\/strong><\/p>\n

1.\u00a0Diana A. Aguilar-Ayala, Laurentijn Tilleman,et al.The transcriptome of Mycobacterium tuberculosis in a lipid-rich dormancy model through RNAseq analysis. scientific reports.2017;7:17665<\/span><\/p>\n

2.\u00a0Giulia Degiacomi, Andrej BenjakJan Madacki, et al. Essentiality of mmpL3 and impact of its silencing on Mycobacterium tuberculosis gene expression.scientific reports. 2017; 7: 43495.<\/span><\/p>\n

 <\/p>\n

\u7ed3\u6838\u8f6c\u5f55\u7ec4\u5b66\u76f8\u5173\u6587\u732e\u5171\u4eab\uff1a<\/span><\/strong><\/p>\n

1. Genome-Wide Transcriptional Responses of Mycobacterium to Antibiotics.<\/span><\/p>\n

2.\u00a0Small RNA profiling in Mycobacterium tuberculosis identifies MrsI as necessary for an anticipatory iron sparing response.<\/span><\/p>\n

3.\u00a0The transcriptome of Mycobacterium tuberculosis in a lipid-rich dormancy model through RNAseq analysis.<\/span><\/p>\n

4.\u00a0Essentiality of mmpL3 and impact of its silencing on Mycobacterium tuberculosis gene expression.<\/span><\/p>\n

 <\/p>\n

\u4e91\u76d8\u5171\u4eab\u94fe\u63a5<\/strong><\/p>\n

\u94fe\u63a5\uff1ahttps:\/\/pan.baidu.com\/s\/1i1-aBSDeK5zwFcnGkYW_tA<\/p>\n

\u63d0\u53d6\u7801\uff1afm3c<\/p>\n

 <\/p>\n

\u8054\u7cfb\u6211\u4eec<\/strong><\/p>\n

\u4e0a\u6d77\u6676\u8bfa\u751f\u7269\u79d1\u6280\u6709\u9650\u516c\u53f8<\/section>\n
\u7535\u8bdd\uff1a0757-29328131-616<\/section>\n
\u624b\u673a\/\u5fae\u4fe1\uff1a13392273325<\/section>\n
Q Q\uff1a2775427010<\/section>\n
\u4e1a\u52a1\uff1ainfo@gene-optimal.com<\/section>\n
\u62db\u8058\uff1ahr@gene-optimal.com<\/section>\n
\u5730\u5740\uff1a\u5e7f\u4e1c\u7701\u4f5b\u5c71\u5e02\u4f5b\u5c71\u65b0\u57ce\u5cad\u5357\u5927\u90532\u53f7\u4e2d\u6b27\u4e2d\u5fc3A\u680b<\/section>\n
\u5b98\u7f51\uff1awww.gene-optimal.com<\/section>\n
\u7248\u6743\u58f0\u660e\uff1a\u672c\u6587\u7cfb\u6676\u8bfa\u751f\u7269\u7f16\u8bd1\u6574\u7406\uff0c\u672a\u7ecf\u6676\u8bfa\u751f\u7269\u6388\u6743\uff0c\u4e0d\u5f97\u8f6c\u8f7d\u548c\u4f7f\u7528\uff0c\u5982\u9700\u83b7\u53d6\u6388\u6743\uff0c\u8bf7\u8054\u7cfb\u6211\u4eec\u3002<\/span><\/section>\n","protected":false},"excerpt":{"rendered":"

\u8f6c\u5f55\u7ec4\u5b66\u662f\u529f\u80fd\u57fa\u56e0\u7814\u7a76\u7684\u91cd\u8981\u7ec4\u6210\u90e8\u5206\uff0c\u662f\u4e00\u95e8\u5728\u6574\u4f53…<\/p>\n","protected":false},"author":1,"featured_media":41695,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[4],"tags":[],"_links":{"self":[{"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/posts\/41679"}],"collection":[{"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/comments?post=41679"}],"version-history":[{"count":0,"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/posts\/41679\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/media\/41695"}],"wp:attachment":[{"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/media?parent=41679"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/categories?post=41679"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/tags?post=41679"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}