{"id":41629,"date":"2019-12-23T08:00:29","date_gmt":"2019-12-23T00:00:29","guid":{"rendered":"https:\/\/www.gene-optimal.com\/?p=41629"},"modified":"2023-12-05T13:18:06","modified_gmt":"2023-12-05T05:18:06","slug":"mod-proteomics-mtb","status":"publish","type":"post","link":"https:\/\/www.gene-optimal.com\/mod-proteomics-mtb\/","title":{"rendered":"\u4fee\u9970\u86cb\u767d\u8d28\u7ec4\u5b66\u5e94\u7528\u4e8e\u7ed3\u6838\u5206\u679d\u6746\u83cc"},"content":{"rendered":"<p><strong>\u4fee\u9970\u86cb\u767d\u8d28\u7ec4\u5b66\uff1a<\/strong>\u5c06\u86cb\u767d\u5b9a\u91cf\u6280\u672f\u4e0e\u86cb\u767d\u8d28\u4fee\u9970\u5bcc\u96c6\u6280\u672f\u76f8\u7ed3\u5408\uff0c\u5728\u9274\u5b9a\u7ffb\u8bd1\u540e\u4fee\u9970\u4f4d\u70b9\u7684\u540c\u65f6\uff0c\u5bf9\u4e0d\u540c\u7684\u6837\u672c\u4e2d\u7ffb\u8bd1\u540e\u4fee\u9970\u7684\u7a0b\u5ea6\u8fdb\u884c\u76f8\u5bf9\u5b9a\u91cf\uff0c\u5b9e\u73b0\u5927\u89c4\u6a21\u7684\u4fee\u9970\u86cb\u767d\u8d28\u7ec4\u5b66\u5b9a\u6027\u548c\u5b9a\u91cf\u5206\u6790\uff0c\u4ece\u800c\u8fdb\u4e00\u6b65\u63ed\u793a\u67d0\u4e9b\u751f\u7406\u3001\u75c5\u7406\u673a\u5236\uff0c\u5bfb\u627e\u751f\u7269\u6807\u5fd7\u7269\u3001\u836f\u7269\u7684\u4f5c\u7528\u9776\u70b9\u7b49\u3002<\/p>\n<p>\u86cb\u767d\u8d28\u7ffb\u8bd1\u540e\u4fee\u9970\u7c7b\u578b\u591a\u6837\uff0c\u76ee\u524d\u5df2\u6709\u62a5\u9053\u5305\u62ec<strong>\u78f7\u9178\u5316\u3001N-\u7cd6\u57fa\u5316\u3001\u4e59\u9170\u5316\u3001\u6cdb\u7d20\u5316\u3001\u7532\u57fa\u5316\u3001\u4e19\u9170\u5316\u3001\u4e19\u4e8c\u9170\u5316\u3001\u620a\u4e8c\u9170\u5316\u3001\u7425\u73c0\u9170\u5316<\/strong>\u7b49\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-41630\" src=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot01.png\" alt=\"\" width=\"835\" height=\"172\" srcset=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot01.png 1330w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot01-150x31.png 150w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot01-300x62.png 300w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot01-768x158.png 768w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot01-1024x211.png 1024w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot01-200x41.png 200w\" sizes=\"(max-width: 835px) 100vw, 835px\" \/><\/p>\n<p style=\"text-align: center;\"><span style=\"color: #3366ff;\"><strong>\u6587\u732e\u89e3\u8bfb\uff1a\u8d56\u6c28\u9178\u4e59\u9170\u57fa\u5316\u5728<em>M.tb<\/em>\u4e2d\u7684\u529f\u80fd<\/strong><\/span><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-41631\" src=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot02.png\" alt=\"\" width=\"736\" height=\"249\" srcset=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot02.png 1144w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot02-150x51.png 150w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot02-300x101.png 300w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot02-768x260.png 768w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot02-1024x346.png 1024w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot02-200x68.png 200w\" sizes=\"(max-width: 736px) 100vw, 736px\" \/><\/p>\n<p>\u86cb\u767d\u8d28\u7684\u8d56\u6c28\u9178\u4e59\u9170\u5316\u662f\u4e00\u79cd\u53ef\u9006\u7684\u7ffb\u8bd1\u540e\u4fee\u9970\uff0c\u5728\u771f\u6838\u751f\u7269\u548c\u539f\u6838\u751f\u7269\u4e2d\u8d77\u7740\u91cd\u8981\u7684\u8c03\u63a7\u4f5c\u7528\u3002\u8d56\u6c28\u9178\u4e59\u9170\u5316\u53ef\u80fd\u5728\u7ed3\u6838\u5206\u679d\u6746\u83cc\u7684\u53d1\u75c5\u673a\u5236\u4e2d\u8d77\u91cd\u8981\u4f5c\u7528\uff0c\u7136\u800c\uff0c\u76ee\u524d\u5df2\u77e5\u7684\u7ed3\u6838\u75c5\u4e59\u9170\u5316\u86cb\u767d\u5f88\u5c11\uff0c\u8fd9\u662f\u7406\u89e3\u53ef\u9006\u8d56\u6c28\u9178\u4e59\u9170\u5316\u5728\u8be5\u75c5\u539f\u4f53\u4e2d\u7684\u529f\u80fd\u4f5c\u7528\u7684\u4e3b\u8981\u969c\u788d\u3002\u8be5\u6587\u7814\u7a76\u4e3b\u8981\u5185\u5bb9\u5982\u4e0b\uff1a<\/p>\n<p>\uff081\uff09\u7ed3\u6838\u5206\u679d\u6746\u83ccH37Ra\u4e59\u9170\u57fa\u5316\u86cb\u767d\u8d28\u7ec4\u5b66\u5206\u6790\u3002<\/p>\n<p>\uff082\uff09\u5728\u8d56\u6c28\u9178\u4e59\u9170\u5316\u8c31\u6570\u636e\u751f\u7269\u4fe1\u606f\u5b66\u5206\u6790\u57fa\u7840\u4e0a\uff0c\u4e3a\u8fdb\u4e00\u6b65\u9a8c\u8bc1\u4e59\u9170\u5316\u4fee\u9970\u5728\u7ec6\u80de\u751f\u7406\u8fc7\u7a0b\u4e2d\u7684\u529f\u80fd\uff0c\u8be5\u7814\u7a76\u901a\u8fc7\u6784\u5efa<em>MRA_1161<\/em>\u57fa\u56e0\uff08\u7f16\u7801\u53bb\u4e59\u9170\u5316\u9176Sir2\u7c7b\u86cb\u767d\uff0c\u76ee\u524d\u7ed3\u6838\u5206\u679d\u6746\u83cc\u4e2d\u552f\u4e00\u5df2\u77e5\u53bb\u4e59\u9170\u5316\u9176\uff09\u7f3a\u5931\u7a81\u53d8\u682a\uff0c\u9a8c\u8bc1\u86cb\u767d\u8d28\u4e59\u9170\u5316\u5bf9\u83cc\u682a\u5f62\u6001\u3001\u751f\u7269\u819c\u5f62\u6210\u3001\u70ed\u6fc0\u53cd\u5e94\u3001\u78b3\u6e90\u5229\u7528\u7684\u5f71\u54cd\u3002<\/p>\n<p>\uff083\uff09\u4e59\u9170\u5316\u5bf9\u70ed\u4f11\u514b\u86cb\u767dHspX\u7684\u514d\u75ab\u539f\u6027\u5f71\u54cd\u3002<\/p>\n<p>&nbsp;<\/p>\n<p style=\"text-align: center;\"><strong>\u7ed3\u6838\u5206\u679d\u6746\u83ccH37Ra\u4e59\u9170\u57fa\u5316\u86cb\u767d\u8d28\u7ec4\u5b66\u5206\u6790<\/strong><\/p>\n<p>\uff081\uff09\u6eb6\u6db2\u548c\u51dd\u80f6\u4e2d\u6d88\u5316\u86cb\u767d\u63d0\u53d6\u7269\uff0c\u5e76\u8fdb\u884c\u8fde\u7eed\u7684\u80f0\u86cb\u767d\u9176\u5904\u7406\u3002<\/p>\n<p>\uff082\uff09\u5c06\u63d0\u53d6\u7684\u86cb\u767d\u8d28\u6d88\u5316\u540e\u7684\u80bd\u6bb5\u5728\u53cd\u76f8C18\u67f1\u4e0a\u8fdb\u884c\u5206\u79bb\u548c\u8131\u76d0\uff0c\u6536\u96c6\u4f4e\u4e30\u5ea6\u7684\u4fee\u9970\u4f4d\u70b9\u3002<\/p>\n<p>\uff083\uff09\u5229\u7528\u6297\u4e59\u9170\u8d56\u6c28\u9178\u6297\u4f53\u5bf9\u8d56\u6c28\u9178\u4e59\u9170\u5316\u80bd\u6bb5\u8fdb\u884c\u514d\u75ab\u5bcc\u96c6\u3002<\/p>\n<p>\uff084\uff09\u7528nanoLC-MS\/MS\u5728\u7535\u55b7\u96fe\u79bb\u5b50\u9631\u8d28\u8c31\u4eea\u4e0a\u8fdb\u884c\u9274\u5b9a\u3002<\/p>\n<p>\uff085\uff09\u5bf9\u539f\u59cb\u6570\u636e\u8fdb\u884c\u5904\u7406\uff0c\u751f\u7269\u4fe1\u606f\u5b66\u5206\u6790\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-41632\" src=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot03.png\" alt=\"\" width=\"276\" height=\"733\" srcset=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot03.png 276w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot03-56x150.png 56w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot03-113x300.png 113w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot03-200x531.png 200w\" sizes=\"(max-width: 276px) 100vw, 276px\" \/><\/p>\n<p><strong>1.\u00a0<\/strong><strong>\u7ed3\u6838\u5206\u679d\u6746\u83ccH37Ra\u4e59\u9170\u57fa\u5316\u86cb\u767d\u8d28\u7ec4\u5b66\u5206\u6790<\/strong><\/p>\n<p>\u5171\u68c0\u6d4b\u5230137\u4e2a\u4e59\u9170\u5316\u86cb\u767d\uff0c\u5171226\u4e2a\u4e59\u9170\u5316\u4f4d\u70b9\u3002\u5176\u4e2din-gel\u68c0\u6d4b72\u4e2a\u4e59\u9170\u5316\u86cb\u767d\uff0cin-solution\u68c0\u6d4b115\u4e2a\u4e59\u9170\u5316\u86cb\u767d\uff0c\u5176\u4e2d\u670950\u4e2a\u4e00\u81f4\u3002in-gel\u68c0\u6d4b105\u4e2a\u4e59\u9170\u5316\u4f4d\u70b9\uff0cin-solution\u68c0\u6d4b187\u4e2a\u4e59\u9170\u5316\u4f4d\u70b9\uff0c\u5176\u4e2d\u670966\u4e2a\u4e00\u81f4\u3002<\/p>\n<p>\u8ba1\u7b97\u4e86\u7ed3\u6838\u5206\u679d\u6746\u83ccH37Ra\u86cb\u767d\u7ec4\u4e2d\u6bcf\u4e2a\u86cb\u767d\u4fee\u9970\u4f4d\u70b9\u7684\u6570\u91cf\uff0c\u4ee5\u8bc4\u4f30\u4e59\u9170\u5316\u4f4d\u70b9\u7684\u5206\u5e03\uff0c\u5176\u4e2d71.53%\u7684\u86cb\u767d\u53ea\u6709\u4e00\u4e2a\u4e59\u9170\u5316\u4f4d\u70b9\u3002\u9ad8\u5ea6\u4e59\u9170\u5316\u86cb\u767d\u8d28\u6709\uff1aGroEL\u3001HspX\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-41633\" src=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot04.png\" alt=\"\" width=\"447\" height=\"849\" srcset=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot04.png 447w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot04-79x150.png 79w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot04-158x300.png 158w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot04-200x380.png 200w\" sizes=\"(max-width: 447px) 100vw, 447px\" \/><\/p>\n<p>\u4e3a\u4e86\u8fdb\u4e00\u6b65\u7814\u7a76\u4e59\u9170\u5316\u86cb\u767d\u7684\u529f\u80fd\u4fe1\u606f\uff0c\u6839\u636eGO\u6ce8\u91ca\u5bf9\u4e59\u9170\u5316\u86cb\u767d\u7684\u751f\u7269\u5b66\u8fc7\u7a0b\u548c\u5206\u5b50\u529f\u80fd\u8fdb\u884c\u4e86\u5206\u7c7b\u3002\u5bf9\u751f\u7269\u5b66\u8fc7\u7a0b\u5bcc\u96c6\u5206\u6790\uff0c\u53d1\u73b0\u86cb\u767d\u8d28\u4e59\u9170\u5316\u4e0e\u4ee5\u4e0bGO\u751f\u7269\u5b66\u8fc7\u7a0b\u5bc6\u5207\u76f8\u5173\uff1a<strong>\u524d\u4f53\u4ee3\u8c22\u7269\u548c\u80fd\u91cf\u7684\u4ea7\u751f\uff0c\u4e59\u9170\u8f85\u9176A\u5206\u89e3\u4ee3\u8c22\u8fc7\u7a0b\uff0c\u67e0\u6aac\u9178\u5faa\u73af\uff0c\u4e59\u9170\u8f85\u9176A\u4ee3\u8c22\u8fc7\u7a0b\uff0c\u8f85\u52a9\u56e0\u5b50\u5206\u89e3\u4ee3\u8c22\u8fc7\u7a0b\uff0c\u8f85\u9176\u964d\u89e3\u8fc7\u7a0b\uff0c\u6709\u6c27\u547c\u5438\uff0c\u7ec6\u80de\u547c\u5438\u7b49\u4ee3\u8c22\u8fc7\u7a0b\u3002<\/strong>\u5bf9\u9274\u5b9a\u7684\u4e59\u9170\u5316\u86cb\u767d\u8fdb\u884cKEGG\u4ee3\u8c22\u9014\u5f84\u5206\u6790\uff0c<strong>\u7ed3\u679c\u8868\u660e\uff0cTCA\u5faa\u73af\u3001\u4e59\u919b\u9178\u548c\u4e8c\u7fa7\u9178\u4ee3\u8c22\u3001\u8102\u80aa\u9178\u4ee3\u8c22\u7b49\u9014\u5f84\u88ab\u663e\u8457\u5bcc\u96c6\uff0c\u56e0\u6b64\u63a8\u6d4b\u4e59\u9170\u5316\u4fee\u9970\u5728TCA\u5faa\u73af\u4e2d\u53d1\u6325\u4e86\u91cd\u8981\u4f5c\u7528\u3002<\/strong><\/p>\n<p><strong>2.\u00a0<\/strong><strong>\u4e59\u9170\u5316\u8d56\u6c28\u9178\u4f4d\u70b9\u5206\u6790<\/strong><\/p>\n<p>\u4e3a\u4e86\u89e3\u4e59\u9170\u5316\u4f4d\u70b9\u5468\u56f4\u6c28\u57fa\u9178\u7684\u5206\u5e03\u89c4\u5f8b\uff0c\u68c0\u67e5\u4e86\u4e59\u9170\u5316\u4f4d\u70b9\u4e24\u4fa7\u768412\u4e2a\u6b8b\u57fa\uff08\u4e0a\u4e0b\u6e38\u54046\u4e2a\uff09\u51fa\u73b0\u9891\u7387\uff0c\u5e76\u7ed8\u5236\u4e86\u70ed\u529b\u578b\u6570\u636e\u56fe\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-41634\" src=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot05.png\" alt=\"\" width=\"315\" height=\"380\" srcset=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot05.png 315w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot05-124x150.png 124w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot05-249x300.png 249w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot05-200x241.png 200w\" sizes=\"(max-width: 315px) 100vw, 315px\" \/><\/p>\n<p>\u6839\u636e\u70ed\u56fe\u5206\u6790\u53d1\u73b0\uff0c\u4e09\u4e2a\u4fa7\u7ffc\u4f4d\u70b9\u6709\u5f88\u5f3a\u7684\u6c28\u57fa\u9178\u504f\u7231\u6027\uff0c\u8c37\u6c28\u9178\u5728-1\u4f4d\u7f6e\u51fa\u73b0\u9891\u7387\u8f83\u9ad8\uff0c\u82ef\u4e19\u6c28\u9178\u5728+1\u4f4d\u7f6e\u51fa\u73b0\u9891\u7387\u8f83\u9ad8\uff0c\u8d56\u6c28\u9178\u5728+3\u4f4d\u7f6e\u51fa\u73b0\u9891\u7387\u8f83\u9ad8\u3002\u7ed3\u6838\u5206\u679d\u6746\u83ccH37Ra\u86cb\u767d\u4e59\u9170\u5316\u4f4d\u70b9\u4e24\u4fa7\u6c28\u57fa\u9178\u6b8b\u57fa\u7684\u5dee\u5f02\u8868\u660e\uff0c\u8fd9\u79cd\u4fee\u9970\u662f\u7531\u4e00\u79cd\u65b0\u7684\u4e59\u9170\u8f6c\u79fb\u9176\u4e9a\u7fa4\u50ac\u5316\u7684\uff0c\u5177\u6709\u72ec\u7279\u7684\u5e95\u7269\u504f\u597d\u3002\u4e59\u9170\u5316\u4f4d\u70b9\u5e38\u53d1\u751f\u5728\u4e8c\u7ea7\u7ed3\u6784\u6709\u5e8f\u7684\u533a\u57df\uff0c\u4e3a\u4e86\u66f4\u8be6\u7ec6\u5730\u7814\u7a76\u4e59\u9170\u5316\u4f4d\u70b9\u5468\u56f4\u86cb\u767d\u5e8f\u5217\u7684\u5c40\u90e8\u4e8c\u7ea7\u7ed3\u6784\uff0c\u6bd4\u8f83\u4e86\u4e59\u9170\u5316\u8d56\u6c28\u9178\u7684\u4e8c\u7ea7\u7ed3\u6784\u4e0e\u6240\u6709\u8d56\u6c28\u9178\u3002\u8fd8\u8bc4\u4f30\u4e86\u4e59\u9170\u5316\u8d56\u6c28\u9178\u4f4d\u70b9\u7684\u6eb6\u5242\u53ef\u53ca\u6027;\u9274\u5b9a\u7684\u4e59\u9170\u5316\u8d56\u6c28\u9178\u4f4d\u70b9\u4e2d\u670984.3%\u66b4\u9732\u4e8e\u86cb\u767d\u8868\u9762\uff0c\u800c\u6240\u6709\u8d56\u6c28\u9178\u6b8b\u57fa\u4e2d\u670978.7%\u66b4\u9732\u4e8e\u86cb\u767d\u8868\u9762\u3002\u8fd9\u4e00\u7ed3\u679c\u8868\u660e\uff0c\u9ad8\u5ea6\u4fdd\u5b88\u7684\u4e59\u9170\u5316\u4f4d\u70b9\u5177\u6709\u5c40\u90e8\u7ed3\u6784\u504f\u597d\uff0c\u66f4\u503e\u5411\u4e8e\u51fa\u73b0\u5728\u03b1-\u87ba\u65cb\u548c\u86cb\u767d\u8d28\u8868\u9762\u3002<\/p>\n<p>\u4e3a\u8bc4\u4f30\u7ed3\u6838\u5206\u679d\u6746\u83cc\u4e59\u9170\u5316\u86cb\u767d\u7684\u8fdb\u5316\u4fdd\u5b88\u6027\uff0c\u7ed3\u6838\u5206\u679d\u6746\u83cc\u7684\u86cb\u767d\u7ec4\u4e0e\u5176\u4ed6231\u4e2a\u7269\u79cd\u86cb\u767d\u7ec4\u8fdb\u884c\u6bd4\u5bf9\uff0c\u7ed3\u679c\u8868\u660e\uff0c\u4e0e\u975e\u4e59\u9170\u5316\u86cb\u767d\u76f8\u6bd4\uff0c\u7ed3\u6838\u5206\u679d\u6746\u83ccH37Ra\u7684\u4e59\u9170\u5316\u86cb\u767d\u5177\u6709\u66f4\u9ad8\u7684\u4fdd\u5b88\u6027\u3002<\/p>\n<p>3.\u00a0<strong>\u4e59\u9170\u5316\u86cb\u767d\u529f\u80fd\u76f8\u4e92\u4f5c\u7528\u7f51\u7edc<\/strong><\/p>\n<p>\u5229\u7528\u86cb\u767d\u8d28\u76f8\u4e92\u4f5c\u7528\u6570\u636e\u5e93\u4e2d\u7684\u86cb\u767d\u8d28\u76f8\u4e92\u4f5c\u7528\u4fe1\u606f\uff0c\u5efa\u7acb\u4e86\u86cb\u767d\u8d28\u76f8\u4e92\u4f5c\u7528\u7f51\u7edc\uff0c\u4ee5\u8bc6\u522b\u5df2\u9274\u5b9a\u7684\u4e59\u9170\u5316\u86cb\u767d\u8d28\u4e4b\u95f4\u7684\u7269\u7406\u548c\u529f\u80fd\u76f8\u4e92\u4f5c\u7528\u3002\u7ed3\u679c\u8868\u660e\uff0c\u5728\u7ed3\u6838\u5206\u679d\u6746\u83cc\u4e2d\uff0c\u86cb\u767d\u7ffb\u8bd1\u53ef\u80fd\u53d7\u8d56\u6c28\u9178\u4e59\u9170\u5316\u7684\u8c03\u63a7\uff0c\u800c\u5927\u591a\u6570\u5df2\u9274\u5b9a\u7684\u4e59\u9170\u5316\u86cb\u767d\u90fd\u53c2\u4e0e\u63a7\u5236\u5173\u952e\u4ee3\u8c22\u8fc7\u7a0b\u7684\u4e2d\u5fc3\u4ee3\u8c22\u901a\u8def\u3002\u5df2\u77e5\u7684\u7ed3\u6838\u5206\u679d\u6746\u83cc\u514d\u75ab\u539fHspX\u4e0e\u5176\u4ed6\u529f\u80fd\u76f8\u5173\u86cb\u767d\u7d27\u5bc6\u76f8\u4e92\u4f5c\u7528\u3002<\/p>\n<p>4.\u00a0<strong>\u4ee3\u8c22\u9176\u7684\u4e59\u9170\u5316\u4fee\u9970<\/strong><\/p>\n<p>\u7ed3\u6838\u5206\u679d\u6746\u83ccH37Ra\u4e2d\u8bb8\u591a\u4e59\u9170\u5316\u86cb\u767d\u53c2\u4e0e\u4e2d\u5fc3\u4ee3\u8c22\u9014\u5f84\uff0c\u8d56\u6c28\u9178\u4e59\u9170\u5316\u9176\u53c2\u4e0e\u7cd6\u9175\u89e3\u3001\u7cd6\u5f02\u751f\u548c\u67e0\u6aac\u9178\u5faa\u73af\uff0c\u5728\u4e2d\u5fc3\u4ee3\u8c22\u9014\u5f84\u4e59\u9170\u5316\u7684\u9176\u4e2d\uff0c\u67098\u79cd\u662f\u67e0\u6aac\u9178\u5faa\u73af\u7684\u7ec4\u6210\u90e8\u5206\uff0c\u4ee5\u53ca2\u79cd\u53c2\u4e0e\u4e86\u4e59\u919b\u9178\u916f\u7684\u4ee3\u8c22\u9014\u5f84\uff0c\u5f02\u67e0\u6aac\u9178\u88c2\u89e3\u9176(ICL1)\u548c\u82f9\u679c\u9178\u5408\u6210\u9176(GlcB)\uff0c\u5728\u7ed3\u6838\u5206\u679d\u6746\u83cc\u4e2d\uff0c\u5f02\u67e0\u6aac\u9178\u88c2\u89e3\u9176\u548c\u82f9\u679c\u9178\u9176\u662f\u8be5\u7ec6\u83cc\u5728\u4f53\u5185\u5b58\u6d3b\u6240\u5fc5\u9700\u7684\u3002<\/p>\n<p>\u9664\u4e86\u67e0\u6aac\u9178\u5faa\u73af\u548c\u4e59\u919b\u9178\u9014\u5f84\u4e2d\u7684\u9176\u5916\uff0c\u5728\u7ed3\u6838\u5206\u679d\u6746\u83cc\u4e2d\u8fd8\u53d1\u73b0\u4e86\u8bb8\u591a\u4e0e\u8102\u80aa\u9178\u4ee3\u8c22\u76f8\u5173\u7684\u9176\u88ab\u4e59\u9170\u5316\uff0c\u5305\u62ec\u4e00\u4e9b\u53c2\u4e0e\u8102\u80aa\u9178\u6c27\u5316\u7684\u9176\u548c\u4e00\u4e9b\u53c2\u4e0e\u8102\u80aa\u9178\u751f\u7269\u5408\u6210\u7684\u9176\u3002\u5728\u7ed3\u6838\u5206\u679d\u6746\u83cc\u4e2d\uff0c\u8102\u80aa\u9178\u53ca\u5176\u4ee3\u8c22\u884d\u751f\u7269\u662f\u7ec6\u80de\u58c1\u590d\u5408\u4f53\u7684\u91cd\u8981\u7ec4\u6210\u90e8\u5206\uff0c\u4e0e\u7ec6\u83cc\u7684\u81f4\u75c5\u6027\u6709\u5173\u3002\u86cb\u767d\u8d28\u7ec4\u6570\u636e\u8868\u660e\u4e59\u9170\u5316\u5728\u7ed3\u6838\u5206\u679d\u6746\u83cc\u7684\u8102\u80aa\u9178\u4ee3\u8c22\u4e2d\u8d77\u7740\u91cd\u8981\u7684\u4f5c\u7528\u3002<\/p>\n<p>&nbsp;<\/p>\n<p style=\"text-align: center;\"><strong>\u7ed3\u6838\u5206\u679d\u6746\u83cc\u8d56\u6c28\u9178\u4e59\u9170\u5316\u86cb\u767d\u751f\u7269\u529f\u80fd\u7814\u7a76<\/strong><\/p>\n<p><strong>1.\u00a0<\/strong><strong>\u6784\u5efa\u53bb\u4e59\u9170\u5316\u9176\u7f16\u7801\u57fa\u56e0\u6572\u9664\u83cc\u682aH37Ra\u2206MRA_1161<\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-41635\" src=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot06.png\" alt=\"\" width=\"644\" height=\"366\" srcset=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot06.png 1050w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot06-150x85.png 150w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot06-300x171.png 300w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot06-768x437.png 768w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot06-1024x582.png 1024w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot06-200x114.png 200w\" sizes=\"(max-width: 644px) 100vw, 644px\" \/><\/p>\n<p style=\"text-align: center;\">MRA_1161\u57fa\u56e0\u6572\u9664\u793a\u610f\u56fe<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-41636\" src=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot07.png\" alt=\"\" width=\"459\" height=\"251\" srcset=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot07.png 459w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot07-150x82.png 150w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot07-300x164.png 300w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot07-200x109.png 200w\" sizes=\"(max-width: 459px) 100vw, 459px\" \/><\/p>\n<p style=\"text-align: center;\">PCR\u9a8c\u8bc1\u6572\u9664\u83cc\u682a\u3001SDS-PAGE\u53ca\u514d\u75ab\u5370\u8ff9<\/p>\n<p style=\"text-align: center;\">lane 1: <em>M.tb<\/em> H37Ra\uff1blane 2: <em>M.tb<\/em> H37Ra \u2206MRA_1161\uff1b<\/p>\n<p style=\"text-align: center;\">lane3: <em>M.tb<\/em> H37Ra \u2206MRA_1161(<em>hyg<\/em>&#8211;<em>sac<\/em>B)<\/p>\n<p>&nbsp;<\/p>\n<p>PCR\u7ed3\u679c\u8868\u660e\uff0cMRA_1161\u57fa\u56e0\u6572\u9664\u7a81\u53d8\u682a\u6784\u5efa\u6210\u529f\u3002\u76ee\u524dMRA_1161\u662f\u552f\u4e00\u5df2\u77e5\u7684\u53bb\u4e59\u9170\u57fa\u5316\u86cb\u767d\u8d28\u7f16\u7801\u57fa\u56e0\uff0c\u4e59\u9170\u5316\u9176\u4ee3\u8c22\u53ef\u9006\u539f\u56e0\uff0c\u514d\u75ab\u5370\u8ff9\u5206\u6790\u7ed3\u679c\u8868\u660eMRA_1161\u57fa\u56e0\u7f3a\u5931\u540e\uff0c\u83cc\u682a\u4e59\u9170\u5316\u8868\u8fbe\u6c34\u5e73\u63d0\u9ad8\u3002<\/p>\n<p><strong>2. <\/strong><strong>\u6572\u9664MRA_1161\u7684\u5f71\u54cd<\/strong><\/p>\n<p><strong>\uff081\uff09\u83cc\u843d\u5f62\u6001\u3001\u751f\u7269\u819c\u7684\u5f62\u6210<\/strong><\/p>\n<p>\u7a81\u53d8\u682a\u4e0e\u91ce\u751f\u682a\u76f8\u6bd4\uff0c\u83cc\u843d\u8868\u9762\u6e7f\u6da6\uff0c\u8936\u76b1\u51cf\u5c11\uff0c\u8d8b\u4e8e\u5e73\u6ed1\u3002\u7ed3\u679c\u8868\u660e\uff0c\u53bb\u4e59\u9170\u5316\u9176\u7f16\u7801\u57fa\u56e0MRA_1161\u7f3a\u5931\u53ef\u5f15\u8d77\u7ed3\u6838\u5206\u679d\u6746\u83cc\u83cc\u843d\u5f62\u6001\u53d1\u751f\u6539\u53d8\u3002\u5728\u6db2\u4f53\u57f9\u517b\u6761\u4ef6\u4e0b\uff0c\u53d1\u73b0\u7a81\u53d8\u682a\u4e0e\u91ce\u751f\u682a\u751f\u7269\u819c\u5f62\u6210\u5b58\u5728\u5dee\u5f02\uff0c\u91ce\u751f\u682a\u4f1a\u5728\u6db2\u4f53\u57f9\u517b\u57fa\u8868\u9762\u5f62\u6210\u4e00\u5c42\u767d\u8272\u8584\u819c\uff0c\u5e76\u6cbf\u74f6\u58c1\u5ef6\u4f38\uff0c\u800c\u7a81\u53d8\u682a\u5f62\u6210\u7684\u8584\u819c\u975e\u5e38\u5fae\u5f31\uff0c\u5e76\u6ca1\u6709\u660e\u663e\u5ef6\u4f38\u3002\u8fd9\u4e00\u7ed3\u679c\u8868\u660e\uff0c\u53bb\u4e59\u9170\u5316\u9176\u7f16\u7801\u57fa\u56e0MRA_1161\u7f3a\u5931\u4f1a\u5bfc\u81f4\u7ed3\u6838\u5206\u679d\u6746\u83cc\u751f\u7269\u819c\u5f62\u6210\u80fd\u529b\u7684\u7f3a\u9677\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-41637\" src=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot08.png\" alt=\"\" width=\"403\" height=\"374\" srcset=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot08.png 672w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot08-150x139.png 150w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot08-300x279.png 300w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot08-200x186.png 200w\" sizes=\"(max-width: 403px) 100vw, 403px\" \/><\/p>\n<p><strong>\uff082\uff09\u70ed\u51fb\u8010\u53d7\u6027<\/strong><\/p>\n<p>\u86cb\u767d\u4fee\u9970\u7ec4\u5b66\u5206\u6790\u663e\u793a\uff0c\u6709\u5927\u91cf\u70ed\u4f11\u514b\u86cb\u767d\u5b58\u5728\u4e59\u9170\u5316\u4fee\u9970\uff0c\u70ed\u4f11\u514b\u86cb\u767d\u4e0e\u7ec6\u83cc\u5bf9\u73af\u5883\u70ed\u538b\u529b\u76f8\u5173\u3002\u5bf9\u7a81\u53d8\u682a\u53ca\u91ce\u751f\u682a\u8fdb\u884c\u70ed\u51fb\u8010\u53d7\u6027\u5206\u6790\u5b9e\u9a8c\uff0c\u5c06\u91ce\u751f\u83cc\u4e0e\u6572\u9664\u83cc\u57f9\u517b\u4e00\u5b9a\u65f6\u95f4\u540e\uff0c53\u2103\u6c34\u6d74\u540e\u7a00\u91ca\u6d82\u677f\uff0c37\u2103\u57f9\u517b25\u5929\u540e\u5bf9\u83cc\u4f53\u8ba1\u6570\u3002\u7ed3\u679c\u5982\u4e0b\u56fe\u6240\u793a\uff0c\u4e0e\u91ce\u751f\u682a\u76f8\u6bd4\uff0c\u7a81\u53d8\u682a\u70ed\u6fc01 h\u5b58\u6d3b\u6570\u76ee\u662f\u91ce\u751f\u682a\u7684100\u500d\u3002\u7ed3\u679c\u8bf4\u660e\uff0c\u8d56\u6c28\u9178\u4e59\u9170\u5316\u53ef\u8c03\u8282\u7ed3\u6838\u5206\u679d\u6746\u83cc\u70ed\u51fb\u53cd\u5e94\uff0c\u4f7f\u83cc\u682a\u66f4\u8010\u53d7\u73af\u5883\u4e2d\u7684\u70ed\u538b\u529b\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-41638\" src=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot09.png\" alt=\"\" width=\"441\" height=\"303\" srcset=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot09.png 654w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot09-150x103.png 150w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot09-300x206.png 300w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot09-200x137.png 200w\" sizes=\"(max-width: 441px) 100vw, 441px\" \/><\/p>\n<p><strong>\uff083\uff09\u78b3\u6e90\u5229\u7528<\/strong><\/p>\n<p>MRA_1161\u76ee\u524d\u552f\u4e00\u5df2\u77e5\u7684\u53bb\u4e59\u9170\u57fa\u5316\u86cb\u767d\u8d28\u7f16\u7801\u57fa\u56e0\uff0c\u4e59\u9170\u5316\u9176\u4ee3\u8c22\u53ef\u9006\u539f\u56e0\uff0c\u57fa\u56e0\u7f3a\u5931\u540e\u4f1a\u5f71\u54cd\u78b3\u6e90\u5229\u7528\u3002\u5f53\u8461\u8404\u7cd6\u6210\u4e3a\u552f\u4e00\u78b3\u6e90\u65f6\uff0c\u7a81\u53d8\u682a\u751f\u957f\u7f13\u6162\uff0c\u5f53\u4e59\u9178\u76d0\u4f5c\u4e3a\u552f\u4e00\u78b3\u6e90\u65f6\uff0cMRA_1161\u6572\u9664\u7a81\u53d8\u682a\u5728\u4e0d\u540c\u6d53\u5ea6\u4e59\u9178\u76d0\u4e0b\u7684\u751f\u957f\u901f\u7387\u65e0\u660e\u663e\u53d8\u5316\u3002\u7ed3\u679c\u8868\u660e\uff0c\u8d56\u6c28\u9178\u4e59\u9170\u5316\u4fee\u9970\u5f71\u54cd\u7ed3\u6838\u5206\u679d\u6746\u83cc\u5bf9\u8461\u8404\u7cd6\u7684\u5229\u7528\uff0c\u5bf9\u918b\u9178\u76d0\u7684\u5229\u7528\u5f71\u54cd\u4e0d\u5927\u3002<\/p>\n<p>&nbsp;<\/p>\n<p style=\"text-align: center;\"><strong>\u4e59\u9170\u5316\u5bf9HspX\u514d\u75ab\u539f\u6027\u5f71\u54cd<\/strong><\/p>\n<p>\u8be5\u7814\u7a76\u4e2d\u4ea7\u751f\u7684\u4e59\u9170\u57fa\u7ec4\u6570\u636e\u663e\u793a\uff0c\u670945\u79cd\u4e59\u9170\u5316\u5206\u6ccc\u86cb\u767d\u53ef\u4ee5\u523a\u6fc0\u5bbf\u4e3b\u7684\u514d\u75ab\u53cd\u5e94\u3002\u56e0\u6b64\uff0c\u68c0\u6d4b\u4e86\u4e59\u9170\u5316\u662f\u5426\u5f71\u54cd\u5206\u6ccc\u86cb\u767d\u7684\u514d\u75ab\u539f\u6027\u3002<\/p>\n<p>45\u79cd\u5206\u6ccc\u86cb\u767d\u4e2d\uff0c\u7ed3\u6838\u5206\u679d\u6746\u83cc\u70ed\u4f11\u514b\u86cb\u767dX (HspX)\u6709\u591a\u4e2a\u4f4d\u70b9\u53d1\u751f\u4e59\u9170\u5316\u3002\u6709\u8bc1\u636e\u8868\u660e\u7ed3\u6838\u5206\u679d\u6746\u83cc\u7684HspX\u5728\u5bbf\u4e3b\u4f53\u5185\u8bf1\u5bfc\u6709\u6548\u7684\u514d\u75ab\u5e94\u7b54\uff0c\u91cd\u7ec4HspX\u86cb\u767d\u514d\u75ab\u5c0f\u9f20\u540e\uff0c\u5e72\u6270\u7d20\u6c34\u5e73\u5347\u9ad8\u3002<\/p>\n<p>\u7531\u4e8e\u96be\u4ee5\u5728\u4f53\u5916\u5236\u5907\u4e59\u9170\u5316\u548c\u53bb\u4e59\u9170\u5316\u5168\u957fHspX\uff0c\u5408\u6210\u4e86\u4e24\u4e2a\u5e8f\u5217\u76f8\u540c\u768430-mer\u80bd\u6bb5(HspX\u6b8b\u57fa62-91)\uff0c\u5176\u4e2d\u4e00\u4e2a\u5e8f\u5217\u7684\u8d56\u6c28\u9178\u6b8b\u57fa\u5168\u90e8\u4e59\u9170\u5316(\u80bd\u6bb5A-6291)\uff0c\u53e6\u4e00\u4e2a\u4e0d\u542b\u4e59\u9170\u5316\uff086291\uff09\u3002<\/p>\n<p>\u6536\u96c626\u4e2a\u75c5\u4eba\u7684\u8840\u6837\uff0cELISPOT\u5206\u6790\u6bd4\u8f83A-6291\u548c6291\u514d\u75ab\u539f\u6027\u3002\u7ed3\u679c\u8868\u660e\uff0c\u5176\u4e2d\u4e09\u4e2a\u6837\u54c1\uff083\u300113\u300125\uff09\u5bf9\u80bd6291\u514d\u75ab\u9633\u6027\uff0c\u800c13\u53f7\u6837\u54c1\u5bf9A-6291\u7684\u514d\u75ab\u539f\u6027\u5f88\u5f31\uff0c\u5176\u5b83\u65e0\u514d\u75ab\u539f\u6027\u3002\u8fd9\u4e9b\u7ed3\u679c\u8868\u660e\uff0c\u4e59\u9170\u5316\u4f5c\u7528\u5f71\u54cd\u5176\u5206\u6ccc\u86cb\u767d\u7684\u514d\u75ab\u539f\u6027\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-41639\" src=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot10.png\" alt=\"\" width=\"1006\" height=\"328\" srcset=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot10.png 1006w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot10-150x49.png 150w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot10-300x98.png 300w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot10-768x250.png 768w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/12\/prot10-200x65.png 200w\" sizes=\"(max-width: 1006px) 100vw, 1006px\" \/><\/p>\n<p style=\"text-align: center;\">a\uff1a\u9634\u6027\u5bf9\u7167 \uff1bb:6291\uff1bc:A-6291 \uff1bd:\u9633\u6027\u5bf9\u7167<\/p>\n<p>&nbsp;<\/p>\n<p><strong>\u5c0f\u7ed3<\/strong><\/p>\n<p>\u8be5\u7814\u7a76\u5229\u7528\u514d\u75ab\u6c89\u6dc0\u548c\u9ad8\u5206\u8fa8\u7387\u8d28\u8c31\u6280\u672f\uff0c\u5bf9\u7ed3\u6838\u5206\u679d\u6746\u83cc\u7684\u4e59\u9170\u5316\u86cb\u767d\u8d28\u53ca\u5176\u8d56\u6c28\u9178\u4e59\u9170\u5316\u4fee\u9970\u4f4d\u70b9\u8fdb\u884c\u4e86\u68c0\u6d4b\uff0c\u5171\u9274\u5b9a\u4e86137\u4e2a\u4e59\u9170\u5316\u86cb\u767d\u4e2d\u7684226\u4e2a\u4e59\u9170\u5316\u4f4d\u70b9\u3002<\/p>\n<p>\u5bf9\u7ed3\u6838\u5206\u679d\u6746\u83cc\u4e59\u9170\u5316\u8c31\u6570\u636e\u8fdb\u884c\u751f\u7269\u4fe1\u606f\u5b66\u5206\u6790\uff0c\u5176\u4e2d\u6709127\u4e2a\u4e59\u9170\u5316\u86cb\u767d\u53c2\u4e0e\u751f\u7406\u548c\u4ee3\u8c22\u8fc7\u7a0b\uff0c\u5305\u62ecTCA\u5faa\u73af\u3001\u8102\u80aa\u9178\u7b49\u57fa\u672c\u7269\u8d28\u7684\u4ee3\u8c22\u3001\u83cc\u4f53\u751f\u957f\u3001\u5e94\u6fc0\u53cd\u5e94\u7b49\uff0c\u5c24\u5176\u662f\u70ed\u4f11\u514b\u86cb\u767d\u5177\u6709\u9ad8\u5ea6\u4e59\u9170\u5316\u4fee\u9970\u3002\u7ed3\u679c\u663e\u793a\uff0c\u4e59\u9170\u5316\u4fee\u9970\u53ef\u80fd\u5728\u78b3\u6e90\u4ee3\u8c22\u3001\u8102\u80aa\u9178\u5408\u6210\u3001\u5e94\u6fc0\u53cd\u5e94\u7b49\u8fc7\u7a0b\u4e2d\u53d1\u6325\u7740\u91cd\u8981\u4f5c\u7528\u3002<\/p>\n<p>\u901a\u8fc7\u566c\u83cc\u4f53\u4ecb\u5bfc\u7684\u540c\u6e90\u91cd\u7ec4\u65b9\u6cd5\uff0c\u6784\u5efa\u7ed3\u6838\u5206\u679d\u6746\u83ccH37Ra\u2206MRA_1161\uff08NAH+\u4f9d\u8d56\u8131\u4e59\u9170\u5316\u9176\uff09\uff0c\u8fdb\u4e00\u6b65\u5bf9\u8d56\u6c28\u9178\u4e59\u9170\u5316\u86cb\u767d\u8d28\u529f\u80fd\u8fdb\u884c\u7814\u7a76\uff0c\u7ed3\u679c\u8868\u660e\u86cb\u767d\u4e59\u9170\u5316\u5f71\u54cd\u7ed3\u6838\u5206\u679d\u6746\u83cc\u7684\u83cc\u843d\u5f62\u6001\u3001\u751f\u7269\u819c\u5f62\u6210\uff0c\u540c\u65f6\u53ef\u63d0\u9ad8\u83cc\u682a\u8010\u53d7\u9ad8\u6e29\u80c1\u8feb\u80fd\u529b\uff0c\u964d\u4f4e\u5bf9\u8461\u7cd6\u7684\u5229\u7528\u3002<\/p>\n<p>ELISPOT\u5b9e\u9a8c\u7ed3\u679c\u8868\u660e\uff0c\u8d56\u6c28\u9178\u4e59\u9170\u5316\u53ef\u4ee5\u6291\u5236\u5df2\u77e5\u514d\u75ab\u539fHspX\u4ea7\u751f\u7684\u80bd\u7684\u514d\u75ab\u539f\u6027\uff0c\u8fd9\u8868\u660e\u8d56\u6c28\u9178\u4e59\u9170\u5316\u5728\u514d\u75ab\u539f\u6027\u4e2d\u8d77\u8c03\u8282\u4f5c\u7528\u3002\u8fd9\u4e9b\u6570\u636e\u96c6\u662f\u7ed3\u6838\u5206\u679d\u6746\u83cc\u8d56\u6c28\u9178\u4e59\u9170\u5316\u529f\u80fd\u5206\u6790\u7684\u91cd\u8981\u8d44\u6e90\uff0c\u5e76\u6709\u52a9\u4e8e\u9610\u660e\u8fd9\u79cd\u5371\u53ca\u751f\u547d\u7684\u75c5\u539f\u4f53\u7684\u6574\u4e2a\u4ee3\u8c22\u7f51\u7edc\u3002<\/p>\n<p>&nbsp;<\/p>\n<p style=\"text-align: center;\"><span style=\"color: #3366ff;\"><strong>\u86cb\u767d\u8d28\u4fee\u9970\u529f\u80fd\u7814\u7a76\u7c7b\u6587\u7ae0\u5206\u4eab<\/strong><\/span><\/p>\n<section><\/section>\n<p><strong>\u86cb\u767d\u8d28\u4e59\u9170\u5316\u4fee\u9970\u529f\u80fd\u7814\u7a76\u76f8\u5173\u6587\u732e\uff1a<\/strong><\/p>\n<p>1.\u00a0Lysine acetylation of DosR regulates the hypoxia response of\u00a0<em>Mycobacterium tuberculosis<\/em>.<\/p>\n<p>2.\u00a0Post-translational Acetylation of MbtA Modulates Mycobacterial Siderophore Biosynthesis.<\/p>\n<p>3.\u00a0Modulation of Central Carbon Metabolism by\u00a0Acetylation of Isocitrate Lyase in\u00a0<em>Mycobacterium tuberculosis<\/em>.<\/p>\n<p>&nbsp;<\/p>\n<p><strong>\u86cb\u767d\u8d28\u78f7\u9178\u5316\u4fee\u9970\u529f\u80fd\u7814\u7a76\u76f8\u5173\u6587\u732e\uff1a<\/strong><\/p>\n<p>1. Target DNA stabilizes\u00a0<em>Mycobacterium tuberculosis<\/em>\u00a0DevR\/DosR phosphorylation by the full-length oxygen sensorsDevS\/DosS and DosT.<\/p>\n<p>2. Mycobacterial Mutagenesis and Drug Resistance Are Controlled by Phosphorylation- and Cardiolipin-MediatedInhibition of the RecA Coprotease.<\/p>\n<p>3. Phosphorylation of\u00a0<em>Mycobacterium tuberculosis<\/em>\u00a0protein tyrosine kinase A PtkA by Ser\/Thr protein kinases.<\/p>\n<p>&nbsp;<\/p>\n<p><strong>\u86cb\u767d\u8d28\u7425\u73c0\u9170\u5316\u4fee\u9970\u529f\u80fd\u7814\u7a76\u76f8\u5173\u6587\u732e\uff1a<\/strong><\/p>\n<p>1. Lysine succinylation of\u00a0<em>Mycobacterium tuberculosis<\/em>\u00a0isocitrate lyase (ICL) fine-tunes the microbial resistance toantibiotics.<\/p>\n<p>2. Systematic Analysis of Mycobacterial Acylation Reveals First Example of Acylation-mediated Regulation of Enzyme Activity of a Bacterial Phosphatase.<\/p>\n<p>&nbsp;<\/p>\n<p>\u94fe\u63a5\uff1ahttps:\/\/pan.baidu.com\/s\/1UcfRKwQWcAJ_wFqym3w42g<br \/>\n\u63d0\u53d6\u7801\uff1aitsc<\/p>\n<p>&nbsp;<\/p>\n<p>\u53c2\u8003\u6587\u732e<\/p>\n[1] Fengying Liu, Mingkun Yang, Xudeng Wang, et al. Acetylome Analysis Reveals Diverse Functions of Lysine Acetylation in\u00a0<em>Mycobacterium tuberculosis<\/em>. 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