{"id":41601,"date":"2019-11-27T16:10:43","date_gmt":"2019-11-27T08:10:43","guid":{"rendered":"https:\/\/www.gene-optimal.com\/?p=41601"},"modified":"2023-12-05T13:18:10","modified_gmt":"2023-12-05T05:18:10","slug":"mtb-resistant","status":"publish","type":"post","link":"https:\/\/www.gene-optimal.com\/mtb-resistant\/","title":{"rendered":"\u7ed3\u6838\u5206\u679d\u6746\u83cc\u8010\u836f\u673a\u5236\u7814\u7a76"},"content":{"rendered":"<section>\u76ee\u524d\uff0c\u8010\u836f\u7ed3\u6838\u83cc\u7684\u4f20\u64ad\u4e0e\u8513\u5ef6\u5df2\u6210\u4e3a\u7ed3\u6838\u75c5\u63a7\u5236\u7684\u96be\u9898\u3002\u7ed3\u6838\u8010\u836f\u673a\u5236\u67093\u79cd\u89c2\u70b9\uff1a<\/section>\n<section>\uff081\uff09\u7ec6\u80de\u58c1\u7ed3\u6784\u4e0e\u7ec4\u5206\u53d8\u5316\uff0c\u4f7f\u7ec6\u80de\u58c1\u901a\u900f\u6027\u6539\u53d8\uff0c\u836f\u7269\u901a\u900f\u6027\u964d\u4f4e\uff0c\u4ea7\u751f\u964d\u89e3\u6216\u706d\u6d3b\u9176\u7c7b\uff0c\u6539\u53d8\u4e86\u836f\u7269\u4f5c\u7528\u9776\u4f4d\u3002<\/section>\n<section>\uff082\uff09\u53ef\u80fd\u5b58\u5728\u8010\u836f\u8d28\u7c92\u6216\u8f6c\u5ea7\u5b50\u4ecb\u5bfc\u7684\u8010\u836f\uff1b\u5728MTB\u4e2d\u5df2\u7ecf\u53d1\u73b0\u4e86\u6d3b\u8dc3\u7684\u836f\u7269\u5916\u6392\u6cf5\u7cfb\u7edf\uff0c\u5916\u6392\u6cf5\u80fd\u5c06\u83cc\u4f53\u5185\u836f\u7269\u6cf5\u51fa\uff0c\u4f7f\u80de\u5185\u836f\u7269\u6d53\u5ea6\u4e0d\u80fd\u6709\u6548\u6291\u5236\u6216\u6740\u6b7b\u5206\u679d\u6746\u83cc\uff0c\u4ece\u800c\u4ea7\u751f\u8010\u836f\u6027\u3002<\/section>\n<section>\uff083\uff09\u7ed3\u6838\u8010\u836f\u6027\u7684\u4ea7\u751f\u591a\u89c1\u4e8e\u5176\u57fa\u56e0\u7ec4\u4e0a\u7f16\u7801\u836f\u7269\u6807\u9776\u7684\u57fa\u56e0\u6216\u836f\u7269\u6d3b\u6027\u76f8\u5173\u7684\u9176\u57fa\u56e0\u7a81\u53d8\u6240\u9020\u6210\u3002\u76ee\u524d\u5bf9\u7ed3\u6838\u6746\u83cc\u8010\u836f\u5206\u5b50\u673a\u5236\u7684\u7814\u7a76\u4e3b\u8981\u96c6\u4e2d\u5728\u5404\u79cd\u836f\u7269\u7684\u4f5c\u7528\u9776\u70b9\u53ca\u76f8\u5173\u57fa\u56e0\u7684\u7a81\u53d8\u4e0a\u3002\u5e38\u89c1\u6297\u7ed3\u6838\u836f\u7269\u8010\u836f\u57fa\u56e0\u53ca\u5bf9\u5e94\u7a81\u53d8\u4f4d\u70b9\u89c1\u4e0b\u8868\uff1a<\/section>\n<section><\/section>\n<section><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-41602 \" src=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med01.png\" alt=\"\" width=\"525\" height=\"603\" srcset=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med01.png 681w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med01-131x150.png 131w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med01-261x300.png 261w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med01-200x230.png 200w\" sizes=\"(max-width: 525px) 100vw, 525px\" \/><\/section>\n<section><\/section>\n<section><\/section>\n<section>\u4eca\u5929\u5c0f\u7f16\u5c31\u548c\u5404\u4f4d\u8001\u5e08\u4e00\u8d77\u5b66\u4e60\u51e0\u7bc7\u5173\u4e8e\u7ed3\u6838\u8010\u836f\u673a\u5236\u7814\u7a76\u7684\u6587\u7ae0\uff0c\u603b\u7ed3\u4e00\u4e0b\u5178\u578b\u7684\u8010\u836f\u673a\u5236\u7814\u7a76\u601d\u8def\u3002<\/section>\n<section><\/section>\n<section><\/section>\n<section><\/section>\n<section><\/section>\n<section><\/section>\n<section><strong>\u6587\u732e1\uff1acycA\u70b9\u7a81\u53d8\u5bfc\u81f4BCG\u5bf9\u73af\u4e1d\u6c28\u9178\u8010\u836f<\/strong><\/section>\n<section><\/section>\n<section><\/section>\n<section><\/section>\n<section><strong>\u4e00\u3001\u7814\u7a76\u601d\u8def<\/strong><\/p>\n<section><\/section>\n<section>1. \u76ee\u7684\u57fa\u56e0\u9009\u62e9\uff1a<em>cycA<\/em><\/section>\n<section>2.\u00a0\u91cd\u7ec4\u83cc\u682a\u6784\u5efa\uff1aBCG-Pasteur::I425\u3001BCG-Pasteur::pYUB412\uff08\u5bf9\u7167\uff09\u3001<em>M.smegmatis<\/em> mc<sup>2<\/sup>155::pYUB412\u3001<em>M.smegmatis<\/em> mc<sup>2<\/sup>155::I425\u3001<em>M.smegmatis<\/em> mc<sup>2<\/sup>155\/pMDcycA<sub>Mtb<\/sub>\u3001<em>M.smegmatis<\/em> mc<sup>2<\/sup> 155\/pMDcycA<sub>Mbov<\/sub>\u3001<em>M. smegmatis<\/em> mc<sup>2<\/sup>155\/pMDcycA<sub>BCG<\/sub><\/section>\n<section>3. \u8010\u836f\u673a\u5236\u7814\u7a76\uff1a\u901a\u8fc7\u5bf9\u4ee5\u4e0a\u91cd\u7ec4\u83cc\u682a\u8fdb\u884c\u73af\u4e1d\u6c28\u9178\uff08DCS\uff09\u836f\u654f\u5b9e\u9a8c\uff0c\u68c0\u6d4b\u5728\u4e0d\u540c\u73af\u4e1d\u6c28\u9178\u6d53\u5ea6\u57f9\u517b\u6761\u4ef6\u4e0b\uff0c\u5404\u91cd\u7ec4\u83cc\u682a\u751f\u957f\u60c5\u51b5\u3002<\/section>\n<p><strong>\u4e8c\u3001\u5b9e\u9a8c\u5185\u5bb9\u4e0e\u7ed3\u679c<\/strong><\/p>\n<section>\u6839\u636e\u62a5\u9053\u63a8\u6d4b\uff0cBCG\u7684<em>cycA<\/em>\u57fa\u56e0\u70b9\u7a81\u53d8\uff08G122S\uff09\u5bfc\u81f4\u8be5\u57fa\u56e0\u529f\u80fd\u635f\u5bb3\uff0c\u4ece\u800c\u5bfc\u81f4BCG\u5bf9DCS\u8010\u836f\u6027\u63d0\u9ad8\u3002<\/section>\n<section><strong>\u00a0<\/strong><\/section>\n<section><strong>\u5728BCG\u548c\u803b\u57a2\u5206\u679d\u6746\u83cc\u4e2d\u8868\u8fbe\u7ed3\u6838\u5206\u679d\u6746\u83cc<em>cycA<\/em>\u57fa\u56e0<\/strong>\uff0c\u6784\u5efa\u7ed3\u6838\u5206\u679d\u6746\u83cc<em>cycA<\/em>\uff08Rv1704c\uff09\u57fa\u56e0\u8868\u8fbe\u83cc\u682a\uff0c\u8f7d\u4f53\u4e3apYUB412\uff08\u6574\u5408\u578b\uff09\uff0c\u7535\u8f6c\u81f3<em>M.bovis<\/em>BCG-Pasteur 1173P2\u548c<em>M.smegmatis <\/em>mc<sup>2<\/sup>155\uff0c\u83b7\u5f97\u91cd\u7ec4\u83cc\u682aBCG-Pasteur::I425\u548c<em>M.smegmatis <\/em>mc<sup>2<\/sup>155::I425\uff0c\u5c06\u8d28\u7c92pYUB412\u7535\u8f6c\u81f3<em>M.bovis<\/em>BCG-Pasteur 1173P2\u548c<em>M.smegmatis <\/em>mc<sup>2<\/sup>155\u4f5c\u4e3a\u5bf9\u7167\uff0c\u83b7\u5f97\u83cc\u682aBCG-Pasteur::pYUB412\uff08\u56fe1-1A\uff09\u4ee5\u53ca<em>M.smegmatis<\/em> mc<sup>2<\/sup>155::pYUB412\u3002<\/section>\n<section><\/section>\n<section>DCS\u836f\u7269\u654f\u611f\u6027\u5206\u6790\u7ed3\u679c\u8868\u660e\uff0c\u4e0e\u5bf9\u7167\u76f8\u6bd4\uff0cDCS\u6d53\u5ea6\u4e3a8\u300116 \u03bcg\/mL\u65f6\uff0cBCG-Pasteur::I425\u7684\u751f\u957f\u660e\u663e\u53d7\u5230\u6291\u5236\uff08\u56fe1-1B\uff09\u3002\u5728DCS\u6d53\u5ea6\u4e3a32 \u03bcg\/mL\u57f9\u517b\uff0c\u4e0e\u5bf9\u7167\u76f8\u6bd4\uff0cBCG-Pasteur::I425\u7684\u751f\u957f\u660e\u663e\u53d7\u5230\u6291\u5236\uff08\u56fe1-1C\uff09\uff0c<em>M.smegmatis<\/em>mc<sup>2<\/sup>155::I425\u73af\u4e1d\u6c28\u9178\u654f\u611f\u6027\u65e0\u660e\u663e\u5dee\u5f02\uff08\u56fe1-1D\u3001E\uff09\u3002<\/section>\n<section><strong>\u00a0<\/strong><\/section>\n<section><strong>\u7ed3\u679c\u8868\u660e\uff0cBCG\u5bf9DCS\u5177\u6709\u8010\u836f\u6027\uff0c\u5728BCG\u4e2d\u8868\u8fbe\u7ed3\u6838\u5206\u679d\u6746\u83cc\u7684<\/strong><strong><em>cycA<\/em><\/strong><strong>\u57fa\u56e0\uff0c\u654f\u611f\u6027\u6062\u590d\u3002<\/strong><\/section>\n<\/section>\n<section><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-41603\" src=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med02.png\" alt=\"\" width=\"693\" height=\"775\" srcset=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med02.png 693w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med02-134x150.png 134w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med02-268x300.png 268w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med02-200x224.png 200w\" sizes=\"(max-width: 693px) 100vw, 693px\" \/><\/section>\n<section>\n<p style=\"text-align: center;\">\u56fe1-1 BCG\u3001\u803b\u57a2\u5206\u679d\u6746\u83cc\u4e2d\u8868\u8fbe<em>cycA<\/em>\u57fa\u56e0\u83cc\u682a\u8010\u836f\u5206\u6790<\/p>\n<p style=\"text-align: center;\">\u6ce8\uff1a\u56feB\u4e2d\u767d\u8272\u4e3aBCG-Pasteur::I425\uff0c\u9ed1\u8272\u4e3aBCG-Pasteur::pYUB412\u3002\u56feC\u65b9\u5f62\u4e3aBCG-Pasteur::I425\uff0c\u83f1\u5f62\u4e3aBCG-Pasteur::pYUB412\u3002\u56feD\u4e2d\u767d\u8272\u4e3a<em>M. smegmatis<\/em> mc<sup>2<\/sup>155::I425\uff0c\u9ed1\u8272\u4e3a<em>M. smegmatis<\/em>mc<sup>2<\/sup>155::pYUB412\u3002\u56feE\u4e2d\u65b9\u5f62\u4e3a<em>M. smegmatis <\/em>mc<sup>2<\/sup>155::I425\uff0c\u83f1\u5f62\u4e3a<em>M.smegmatis<\/em>mc<sup>2<\/sup>155::pYUB412\u3002<\/p>\n<p><strong>\u5728\u803b\u57a2\u5206\u679d\u6746\u83cc\u4e2d\u5206\u522b\u8fc7\u8868\u8fbe<em>M.tb<\/em>\u3001<em>M.boiv<\/em>\u3001BCG\u7684<em>cycA<\/em>\u57fa\u56e0<\/strong>\uff0c\u8f7d\u4f53\u4e3apMD31\uff08\u591a\u62f7\u8d1d\uff09\uff0c\u5176\u4e2dBCG\u7684<em>cycA<\/em>\u57fa\u56e0\u4e3a\u7f3a\u9677\u578b\uff0c<em>M.tb<\/em>\u548c<em>M.boiv<\/em>\u7684cycA\u57fa\u56e0\u4e3a\u529f\u80fd\u578b\u3002\u5bf9\u91cd\u7ec4\u83cc\u682a\u836f\u654f\u6027\u5206\u6790\u7ed3\u679c\u663e\u793a\uff0c\u8fc7\u8868\u8fbe<em>M.tb<\/em>\u548c<em>M.boiv<\/em>\u7684<em>cycA<\/em>\u57fa\u56e0\u83cc\u682a\u836f\u7269\u654f\u611f\u6027\u63d0\u9ad8\uff0c\u800c\u8fc7\u8868\u8fbeBCG\u7684<em>cycA<\/em>\u57fa\u56e0\u83cc\u682a\u7684\u836f\u7269\u654f\u611f\u6027\u4e0e\u5bf9\u7167\u4e00\u81f4\uff0c\u672a\u89c1\u83cc\u682a\u5bf9\u73af\u4e1d\u6c28\u9178\u836f\u7269\u654f\u611f\u6027\u660e\u663e\u63d0\u9ad8\uff08\u56fe1-2A\uff09\u3002DCS\u6d53\u5ea6\u4e3a200 \u03bcg\/mL\u60c5\u51b5\u4e0b\uff0c\u4e0e\u5bf9\u7167\u76f8\u6bd4\uff0c<em>M.smegmatis<\/em>mc<sup>2<\/sup>155\/pMDcycA<sub>Mtb<\/sub>\u3001<em>M.smegmatis <\/em>mc<sup>2<\/sup>155\/pMDcycA<sub>Mboiv<\/sub>\u83cc\u682a\u751f\u957f\u660e\u663e\u53d7\u5230\u6291\u5236\uff08\u56fe1-2B\uff09\u3002<\/p>\n<\/section>\n<section><strong>\u00a0<\/strong><\/section>\n<section><strong>\u7ed3\u679c\u8868\u660e\uff0c<em>cycA<\/em>\u57fa\u56e0\u8868\u8fbe\u91cf\u63d0\u9ad8\uff0c\u5bf9DCS\u654f\u611f\u6027\u63d0\u9ad8\uff0c\u4e14BCG\u7684<em>cycA<\/em>\u57fa\u56e0\u7a81\u53d8\u5bfc\u81f4\u5176\u7f16\u7801\u4ea7\u7269\u5931\u53bb\u6d3b\u6027\u3002<\/strong><\/section>\n<section><\/section>\n<section><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-41604\" src=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med03.png\" alt=\"\" width=\"699\" height=\"327\" srcset=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med03.png 699w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med03-150x70.png 150w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med03-300x140.png 300w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med03-200x94.png 200w\" sizes=\"(max-width: 699px) 100vw, 699px\" \/><\/section>\n<section>\n<p style=\"text-align: center;\"><span style=\"font-size: 10pt;\">\u56fe1-2\u803b\u57a2\u8fc7\u8868\u8fbe\u83cc\u682a\u5bf9DCS\u654f\u611f\u6027\u5b9e\u9a8c<\/span><\/p>\n<p style=\"text-align: center;\">\u6ce8\uff1a\u9ed1\u8272\u4e3a<em>M. smegmatis mc<sup>2<\/sup>155<\/em>\/pMD31\uff08\u5bf9\u7167\uff09\uff0c\u767d\u8272\u4e3a<em>M. smegmatis <\/em>mc<sup>2<\/sup>155\/pMDcycA<sub>Mtb<\/sub>\uff0c\u6d45\u7070\u4e3a<em>M. smegmatis <\/em>mc<sup>2<\/sup>155\/pMDcycA<sub>Mbov<\/sub>\uff0c\u6df1\u7070\u4e3a<em>M. smegmatis <\/em>mc<sup>2<\/sup>155\/pMDcycA<sub>BCG<\/sub>\u3002<\/p>\n<p>&nbsp;<\/p>\n<p><strong>\u6587\u732e2\uff1a\u5168\u57fa\u56e0\u7ec4\u6d4b\u5e8f\u4e0e\u8010\u836f\u673a\u5236\u7814\u7a76<\/strong><\/p>\n<p><strong>\u4e00\u3001\u7814\u7a76\u601d\u8def<\/strong><\/p>\n<section>1. \u76ee\u7684\u57fa\u56e0\u9009\u62e9\uff1a<em>ald<\/em>\uff08Rv2780\uff09<\/section>\n<section>2. \u91cd\u7ec4\u83cc\u682a\u6784\u5efa\uff1a<em>ald<\/em>\u57fa\u56e0\u6572\u9664\u83cc\u682a\u2206<em>ald<\/em>\u3001\u56de\u8865\u83cc\u682a\u2206<em>ald<\/em>-comp\u3001BCG-\u2206<em>ald<\/em>-comp\u3002<\/section>\n<section>3. \u8010\u836f\u673a\u5236\u7814\u7a76\uff1a\u4e3a\u786e\u5b9a\u65b0\u7684\u8010\u836f\u57fa\u56e0\u578b\uff0c\u9996\u5148\u5bf9498\u682a\u836f\u654f\u83cc\u682a\u8fdb\u884c\u5168\u57fa\u56e0\u7ec4\u6d4b\u5e8f\u53ca\u8868\u578b\u8010\u836f\u5206\u6790\uff0c\u7ed3\u5408\u8fdb\u5316\u5206\u6790\uff0c\u7b5b\u9009\u76f8\u5173\u57fa\u56e0\uff0c\u6784\u5efa\u91cd\u7ec4\u83cc\u682a\u540e\u8fdb\u884c\u836f\u654f\u5b9e\u9a8c\uff0c\u5206\u6790\u5404\u83cc\u682a\u8010\u836f\u6027\u3002<\/section>\n<p><strong>\u4e8c\u3001\u5b9e\u9a8c\u5185\u5bb9\u4e0e\u7ed3\u679c<\/strong><\/p>\n<section>\u5c06498\u682a\u6765\u81ea\u4e2d\u56fd\u548c\u5357\u975e\u7684\u7ed3\u6838\u5206\u679d\u6746\u83cc\uff0c\u901a\u8fc7\u8868\u578b\u8010\u836f\u5206\u6790\u4e0e\u6d4b\u5e8f\u7ed3\u679c\u8fdb\u884c\u6bd4\u5bf9\uff0c\u7ed3\u679c\u5982\u8868\u683c2-1\u6240\u793a\uff0c\u8868\u578b\u8010\u836f\u4e0e\u57fa\u56e0\u7a81\u53d8\u673a\u5236\u8010\u836f\u4e0d\u5bf9\u5e94\u7684\u6570\u91cf\u8f83\u5c11\uff0c\u63a8\u6d4b\u57fa\u4e8e\u4e2a\u4f53\u7a81\u53d8\u7684\u65b0\u8010\u836f\u673a\u5236\u53ef\u80fd\u6bd4\u8f83\u5c11\u89c1\u3002<\/section>\n<p style=\"text-align: center;\"><span style=\"font-size: 10pt;\">\u88682-1 \u83cc\u682a\u8010\u836f\u6027\u5206\u6790<\/span><\/p>\n<section><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-41605\" src=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med05.png\" alt=\"\" width=\"448\" height=\"253\" srcset=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med05.png 448w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med05-150x85.png 150w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med05-300x169.png 300w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med05-200x113.png 200w\" sizes=\"(max-width: 448px) 100vw, 448px\" \/><\/section>\n<section>\u65b0\u8010\u836f\u57fa\u56e0\u578b\u5206\u6790\uff0c\u53bb\u9664\u5df2\u77e5\u8010\u836f\u57fa\u56e0\u578b\uff0c\u572825\u682a\u5357\u975e\u83cc\u682a\u4e2d\u9274\u5b9a\u4e8611\u79cd\u4e0d\u540c\u7684<em>ald<\/em>\uff08Rv2780\uff09\u57fa\u56e0\u529f\u80fd\u7f3a\u5931\u7a81\u53d8\uff08\u56fe2-1\uff09\uff0c\u8be5\u57fa\u56e0\u7684\u529f\u80fd\u7f3a\u5931\u53ef\u80fd\u4e0e\u8010\u836f\u6027\u76f8\u5173\u3002<\/section>\n<section><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-41606\" src=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med06.png\" alt=\"\" width=\"601\" height=\"449\" srcset=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med06.png 601w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med06-150x112.png 150w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med06-300x224.png 300w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med06-120x90.png 120w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med06-200x149.png 200w\" sizes=\"(max-width: 601px) 100vw, 601px\" \/><\/section>\n<p style=\"text-align: center;\"><span style=\"font-size: 10pt;\">\u56fe2-1 \u7ed3\u6838\u5206\u679d\u6746\u83ccald\u57fa\u56e0\u529f\u80fd\u7f3a\u5931\u7a81\u53d8\u7684\u8d8b\u5316\u8fdb\u5316<\/span><\/p>\n<section><em>ald<\/em>\u57fa\u56e0\u7f16\u7801L-\u4e19\u6c28\u9178\u8131\u6c22\u9176\uff0c\u662f\u4e19\u6c28\u9178\u4ee3\u8c22\u9014\u5f84\u7684\u7ec4\u6210\u90e8\u5206\uff0c\u5176\u4e2d\u5305\u62ec\u4e8c\u7ebf\u836f\u7269\u73af\u4e1d\u6c28\u9178\u7684\u9776\u6807\u3002\u4e3a\u4e86\u786e\u5b9a\u662f\u5426\u56e0\u4e3a<em>ald<\/em>\u57fa\u56e0\u529f\u80fd\u4e27\u5931\u5bfc\u81f4\u5bf9\u73af\u4e1d\u6c28\u9178\u4ea7\u751f\u8010\u836f\u6027\uff0c\u6784\u5efa<em>ald<\/em>\u57fa\u56e0\u6572\u9664\u83cc\u682a\u2206<em>ald<\/em>\uff08CDC1551\u73af\u4e1d\u6c28\u9178\u654f\u611f\u83cc\u682a\uff09\uff0c\u56de\u8865\u83cc\u682a\u2206<em>ald<\/em>-comp\uff0cBCG\u5bf9\u73af\u4e1d\u6c28\u9178\u8010\u836f\uff0c\u4e14<em>ald<\/em>\u57fa\u56e0\u529f\u80fd\u7f3a\u5931\uff0c\u6784\u5efa<em>ald<\/em>\u5728BCG\u4e2d\u7684\u56de\u8865\u83cc\u682aBCG-\u2206<em>ald<\/em>-comp\u3002<\/section>\n<section>\u901a\u8fc7\u5bf9\u4ee5\u4e0a\u83cc\u682a\u8fdb\u884c\u8010\u836f\u6027\u5206\u6790\uff0c\u53d1\u73b0<em>ald<\/em>\u57fa\u56e0\u529f\u80fd\u7f3a\u5931\u7684<em>M.tb<\/em>\u5bf9\u73af\u4e1d\u6c28\u9178\u8010\u836f\u6027\u63d0\u9ad8\uff0c\u7136\u800cBCG\u7684<em>ald<\/em>\u57fa\u56e0\u56de\u8865\u83cc\u682a\u4e0eBCG\u76f8\u6bd4\uff0c\u8010\u836f\u6027\u6ca1\u6709\u660e\u663e\u53d8\u5316\uff08\u88682-2\uff09\u3002<\/section>\n<section>\u5bf9\u4ee5\u4e0a\u83cc\u682a\u5728\u4e0d\u540c\u6d53\u5ea6\u73af\u4e1d\u6c28\u9178\u73af\u5883\u4e0b\u57f9\u517b\uff0c\u68c0\u6d4b\u83cc\u682a\u751f\u957f\u60c5\u51b5\uff0c\u7ed3\u679c\u8868\u660e\uff0c7.5 \u03bcg\/mL\u7684\u73af\u4e1d\u6c28\u9178\u6d53\u5ea6\u4e0b\uff0c\u76f8\u8f83\u4e8e\u91ce\u751f\u578b\u83cc\u682a\uff0c\u2206<em>ald<\/em>\u83cc\u682a\u6709\u663e\u8457\u7684\u751f\u957f\u4f18\u52bf\uff0c\u5c06\u6d53\u5ea6\u63d0\u9ad8\u81f315 \u03bcg\/mL\uff0c\u2206<em>ald<\/em>\u83cc\u682a\u4f9d\u65e7\u6709\u663e\u8457\u7684\u751f\u957f\u4f18\u52bf\uff0c\u4f46\u751f\u957f\u6162\u4e8eBCG\uff08\u56fe2-2\uff09\u3002<\/section>\n<section><strong>\u00a0<\/strong><\/section>\n<section><strong>\u6839\u636e\u836f\u654f\u5206\u6790\uff0c\u8bc1\u5b9e<\/strong><strong><em>ald<\/em>\u57fa\u56e0\u529f\u80fd\u7f3a\u5931\u5bfc\u81f4\u83cc\u682a\u8010\u836f\uff0c\u4f46\u8010\u836f\u6027\u4f4e\u4e8eBCG\u3002<\/strong><\/section>\n<p style=\"text-align: center;\"><span style=\"font-size: 10pt;\">\u88682-2 ald\u529f\u80fd\u7f3a\u5931\u836f\u654f\u6027\u5206\u6790<\/span><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-41608\" src=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med08.png\" alt=\"\" width=\"485\" height=\"204\" srcset=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med08.png 578w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med08-150x63.png 150w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med08-300x126.png 300w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med08-200x84.png 200w\" sizes=\"(max-width: 485px) 100vw, 485px\" \/><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-41609\" src=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med09.png\" alt=\"\" width=\"361\" height=\"287\" srcset=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med09.png 361w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med09-150x119.png 150w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med09-300x239.png 300w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med09-200x159.png 200w\" sizes=\"(max-width: 361px) 100vw, 361px\" \/><\/p>\n<p style=\"text-align: center;\"><span style=\"font-size: 10pt;\">\u56fe2-2 \u91cd\u7ec4\u83cc\u682a\u751f\u957f\u60c5\u51b5<\/span><\/p>\n<\/section>\n<section class=\"\" data-tools=\"135\u7f16\u8f91\u5668\" data-id=\"93104\">\n<section>\n<section class=\"\">\n<section class=\"\" data-brushtype=\"text\"><strong>\u6587\u732e3\uff1aSig B\u8c03\u63a7PABA\u5408\u6210\u9014\u5f84\u4ece\u800c\u5bfc\u81f4PAS\u654f\u611f\u6027\u53d8\u5316\uff08\u975e\u7a81\u53d8\uff09<\/strong><\/section>\n<\/section>\n<\/section>\n<\/section>\n<p><strong>\u4e00\u3001\u7814\u7a76\u601d\u8def\uff1a<\/strong><\/p>\n<section>1. \u76ee\u7684\u57fa\u56e0\u9009\u62e9\uff1a<em>sigma B<\/em><\/section>\n<section>2. \u91cd\u7ec4\u83cc\u682a\u6784\u5efa\uff1a\u6784\u5efa<em>sigma B<\/em>\u57fa\u56e0\u6572\u9664\u83cc\u682aH37Ra \u2206<em>sigB<\/em>\u3001\u56de\u8865\u83cc\u682aH37Ra \u2206<em>sigB<\/em> pMV261::<em>sigB<\/em><em>\u3001<\/em><em>pabB<\/em>\u8fc7\u8868\u8fbe\u83cc\u682aH37RapMV261::<em>pabB<\/em>\u3001<em>pabC<\/em>\u57fa\u56e0\u6572\u9664\u83cc\u682aH37Ra \u2206<em>pabC<\/em><\/section>\n<section>3. \u8010\u836f\u673a\u5236\u7814\u7a76\uff1a\u836f\u654f\u6027\u5206\u6790\u68c0\u6d4b\u7a81\u53d8\u682a\u5bf9\u6297\u7ed3\u6838\u836f\u7269\u654f\u611f\u6027\uff1b\u8f6c\u5f55\u7ec4\u5b66\u5206\u6790\u3001qRT-PCR\u3001WB\u68c0\u6d4b\u6572\u9664\u7a81\u53d8\u682a\u4e0e\u91ce\u751f\u83cc\u682a\u4e4b\u95f4\u5404\u57fa\u56e0\u8868\u8fbe\u6c34\u5e73\u5dee\u5f02\u3002<\/section>\n<p><strong>\u4e8c\u3001\u7814\u7a76\u5185\u5bb9\u4e0e\u7ed3\u679c<\/strong><\/p>\n<section>sigB\u8c03\u63a7pabB\u57fa\u56e0\u8868\u8fbe\uff0c\u4ece\u800c\u5f71\u54cdPABA\u7684\u5408\u6210\uff0cPABA\u7684\u5408\u6210\u4e2d\u65ad\u7684\u4f1a\u5bfc\u81f4\u83cc\u682a\u5bf9\u53f6\u9178\u7c7b\u836f\u7269PAS\u836f\u7269\u654f\u611f\u6027\u63d0\u9ad8\u3002\u6784\u5efa\u6572\u9664\u83cc\u682aH37Ra \u2206<em>sigB<\/em>\u548c\u56de\u8865\u83cc\u682aH37Ra \u2206<em>sigB<\/em> pMV261::<em>sigB<\/em>\uff0c\u68c0\u6d4b\u91ce\u751f\u578b\u83cc\u682a\u548c\u91cd\u7ec4\u83cc\u682a\u5bf9\u5404\u79cd\u6297\u7ed3\u6838\u836f\u7269\u7684MIC\u3002<\/section>\n<section><strong>\u00a0<\/strong><\/section>\n<section><strong>\u83cc\u682aH37Ra \u2206<em>sigB<\/em>\u5bf9\u6297\u7ed3\u6838\u836f\u7269\u654f\u611f\u6027\u589e\u52a0<\/strong>\uff08\u88683-1\uff09\uff0c<strong>\u8fc7\u8868\u8fbepabB\u57fa\u56e0\u83cc\u682a\u5bf9PAS\u7684MIC\u63d0\u9ad8\uff0c\u5bf9PAS\u4ea7\u751f\u4e86\u8010\u836f\u6027<\/strong>\uff08\u88683-2\uff09\u3002<\/section>\n<p style=\"text-align: center;\"><span style=\"font-size: 10pt;\">\u88683-1 \u68c0\u6d4bH37Ra \u2206<em>sigB<\/em>\u3001H37Ra \u2206<em>sigB<\/em> pMV261::<em>sigB<\/em>\u3001H37Ra\u5404\u79cd\u7ed3\u6838\u836f\u7269MIC<\/span><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-41610\" src=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med10.png\" alt=\"\" width=\"655\" height=\"248\" srcset=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med10.png 816w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med10-150x57.png 150w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med10-300x114.png 300w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med10-768x291.png 768w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med10-200x76.png 200w\" sizes=\"(max-width: 655px) 100vw, 655px\" \/><\/p>\n<p style=\"text-align: center;\"><span style=\"font-size: 10pt;\">\u88683-2 \u68c0\u6d4b\u4e0d\u540c\u83cc\u682a\u5bf9PAS\u7684MIC<\/span><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-41611\" src=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med11.png\" alt=\"\" width=\"661\" height=\"119\" srcset=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med11.png 661w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med11-150x27.png 150w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med11-300x54.png 300w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med11-200x36.png 200w\" sizes=\"(max-width: 661px) 100vw, 661px\" \/><\/p>\n<p>\u6db2\u4f53\u57f9\u517bWT\u3001\u6572\u9664\u83cc\u682a\u3001\u56de\u8865\u83cc\u682a\uff0c\u52a0\u51650.4mg\/mL\u7684PAS\uff0c\u57f9\u517b\u81f3\u7b2c\u56db\u5929\uff0cH37Ra \u2206<em>sigB<\/em>\u4e0b\u964d\u81f35*10<sup>3<\/sup>\uff0c\u800c\u91ce\u751f\u53ca\u56de\u8865\u7684CFU\u4e3a5*10<sup>5<\/sup>\uff0cH37Ra \u2206<em>sigB<\/em>\u57f9\u517b\u81f38\u5929\u6025\u5267\u51cf\u5c11\uff08\u56fe3-1\uff09\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-41612\" src=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med12.png\" alt=\"\" width=\"437\" height=\"338\" srcset=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med12.png 666w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med12-150x116.png 150w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med12-300x232.png 300w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med12-200x155.png 200w\" sizes=\"(max-width: 437px) 100vw, 437px\" \/><\/p>\n<p style=\"text-align: center;\"><span style=\"font-size: 10pt;\">\u56fe3-1 PAS\u5bf9<em>sigB<\/em>\u57fa\u56e0\u7f3a\u5931\u7ed3\u6838\u83cc\u682a\u7684\u5f71\u54cd<\/span><\/p>\n<p>&nbsp;<\/p>\n<section><strong>\u8f6c\u5f55\u7ec4\u5b66\u5206\u6790<\/strong>\uff0c\u6572\u9664\u7a81\u53d8\u682a\u4e0e\u91ce\u751f\u578b\u83cc\u682a\u5404\u57fa\u56e0\u8868\u8fbe\u6c34\u5e73\u5dee\u5f02\uff0c\u5176\u4e2d175\u4e2a\u57fa\u56e0\u6807\u6ce8\u4e3a\u663e\u8457\u8c03\u63a7\uff0c33\u4e2a\u4e0a\u8c03\uff0c142\u4e2a\u4e0b\u8c03\u3002\u5176\u4e2d\u4e0ePABA\u5408\u6210\u76f8\u5173\u57fa\u56e0<em>pabB<\/em>\u57fa\u56e0\u4e0b\u8c03\uff08\u88683-3\uff09\u3002<\/section>\n<section><\/section>\n<p style=\"text-align: center;\"><span style=\"font-size: 10pt;\">\u88683-3 \u8f6c\u5f55\u7ec4\u5b66\u5206\u6790\u5404\u57fa\u56e0\u8f6c\u5f55\u6c34\u5e73\u5dee\u5f02<\/span><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-41613\" src=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med13.png\" alt=\"\" width=\"667\" height=\"698\" srcset=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med13.png 667w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med13-143x150.png 143w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med13-287x300.png 287w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med13-200x209.png 200w\" sizes=\"(max-width: 667px) 100vw, 667px\" \/><\/p>\n<section>\u5206\u522b\u91c7\u7528RT-PCR\u5206\u6790\u6572\u9664\u7a81\u53d8\u682a\u4e0e\u91ce\u751f\u83cc\u682a<em>pabB<\/em>\u57fa\u56e0\u8f6c\u5f55\u6c34\u5e73\u5dee\u5f02\uff0c\u91c7\u7528WB\u68c0\u6d4b\u6572\u9664\u7a81\u53d8\u682a\u4e0e\u91ce\u751f\u83cc\u682a<em>pabB<\/em>\u8868\u8fbe\u6c34\u5e73\u5dee\u5f02\uff0c\u7ed3\u679c\u8868\u660e\uff0c\u4e0e\u8f6c\u5f55\u7ec4\u5b66\u5206\u6790\u7ed3\u679c\u4e00\u81f4\uff0c\u6572\u9664\u7a81\u53d8\u682a<em>pabB<\/em>\u57fa\u56e0\u8f6c\u5f55\u6c34\u5e73\u660e\u663e\u4f4e\u4e8e\u91ce\u751f\u578b\u83cc\u682a\uff08\u56fe3-2A\uff09\uff0c\u6572\u9664\u7a81\u53d8\u682a<em>pabB<\/em>\u8868\u8fbe\u6c34\u5e73\u4e5f\u660e\u663e\u4f4e\u4e8e\u91ce\u751f\u578b\u83cc\u682a\uff08\u56fe3-2B\uff09\u3002<\/section>\n<section><strong>\u00a0<\/strong><\/section>\n<section><strong>\u4ee5\u4e0a\u7ed3\u679c\u8868\u660e\uff0c<em>sigB<\/em>\u57fa\u56e0\u53ef\u901a\u8fc7\u8c03\u63a7<em>pabB<\/em>\u57fa\u56e0\u8868\u8fbe\uff0c\u4ece\u800c\u5f71\u54cdPABA\u5408\u6210\u9014\u5f84\uff0c\u5bfc\u81f4\u83cc\u682a\u5bf9PAS\u836f\u654f\u6027\u53d1\u751f\u53d8\u5316\u3002<\/strong><\/section>\n<section><\/section>\n<section><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-41614\" src=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med14.png\" alt=\"\" width=\"294\" height=\"384\" srcset=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med14.png 532w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med14-115x150.png 115w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med14-230x300.png 230w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med14-200x261.png 200w\" sizes=\"(max-width: 294px) 100vw, 294px\" \/><\/section>\n<p style=\"text-align: center;\">\u56fe3-2 \u6572\u9664<em>sigA<\/em>\u5bf9<em>pabB<\/em>\u7684\u5f71\u54cd<\/p>\n<p>&nbsp;<\/p>\n<p><strong>\u6839\u636e\u4ee5\u4e0a\u7684\u4e09\u7bc7\u6587\u732e\u5206\u6790\uff0c\u6211\u4eec\u53d1\u73b0\uff0c\u9488\u5bf9\u8010\u836f\u673a\u5236\u7684\u7814\u7a76\u5927\u4f53\u601d\u8def\u4e3a\uff1a<\/strong><\/p>\n<p><strong>\uff081\uff09\u6839\u636e\u6587\u732e\u62a5\u9053\u6216\u5168\u57fa\u56e0\u7ec4\u6d4b\u5e8f\u5206\u6790\u9009\u62e9\u76ee\u7684\u57fa\u56e0\uff08\u7a81\u53d8\u5bfc\u81f4\u8010\u836f\u6216\u7814\u7a76\u8c03\u63a7\u9776\u6807\u57fa\u56e0\u8868\u8fbe\u5bfc\u81f4\u8010\u836f\uff09\uff1b<\/strong><\/p>\n<p><strong>\uff082\uff09\u5bf9\u76ee\u6807\u83cc\u682a\u8fdb\u884c\u9057\u4f20\u64cd\u4f5c\uff08\u57fa\u56e0\u6572\u9664\u3001\u56de\u8865\u3001\u8fc7\u8868\u8fbe\u3001\u70b9\u7a81\u53d8\uff09\uff0c\u83b7\u5f97\u91cd\u7ec4\u83cc\u682a\uff1b<\/strong><\/p>\n<p><strong>\uff083\uff09\u5bf9\u4ee5\u4e0a\u91cd\u7ec4\u83cc\u682a\u836f\u7269\u654f\u611f\u6027\u5206\u6790\uff0c\u5206\u6790\u8010\u836f\u673a\u5236\u3002<\/strong><\/p>\n<p>&nbsp;<\/p>\n<p style=\"text-align: center;\"><strong>\u5bf9\u8010\u836f\u673a\u5236\u7684\u7814\u7a76\u601d\u8def\u53ef\u6982\u62ec\u4e3a\uff1a<\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-41615\" src=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med15.png\" alt=\"\" width=\"431\" height=\"477\" srcset=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med15.png 711w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med15-136x150.png 136w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med15-271x300.png 271w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/11\/med15-200x221.png 200w\" sizes=\"(max-width: 431px) 100vw, 431px\" \/><\/p>\n<p>&nbsp;<\/p>\n<p><span style=\"font-size: 8pt;\">\u53c2\u8003\u6587\u732e\uff1a<\/span><\/p>\n<ol>\n<li><span style=\"font-size: 8pt;\">Chen JM, Uplekar S, Gordon SV, Cole ST.A Point Mutation in cycA Partially Contributes to the Dcycloserine Resistance Trait of <em>Mycobacterium bovis<\/em> BCG Vaccine Strains.\u00a0PLoS One.2012;7(8):e43467.<\/span><\/li>\n<li><span style=\"font-size: 8pt;\">Desjardins CA, Cohen KA, Munsamy A, et al., Genomic and functional analyses of<em>Mycobacterium tuberculosis<\/em> strains implicate ald in d-cycloserineresistance.\u00a0Nat Genet.\u00a02016May;48(5):544-51.<\/span><\/li>\n<li><span style=\"font-size: 8pt;\">Yang SS, Hu YB, Wang XD, et al., Deletion of sigB Causes Increased Sensitivity to para-Aminosalicylic Acid and Sulfamethoxazole in <em>Mycobacterium tuberculosis<\/em>.\u00a0Antimicrob Agents Chemother.\u00a02017 Sep 22;61(10). pii: e00551-17.<\/span><\/li>\n<\/ol>\n<section><\/section>\n<section>\u8054\u7cfb\u6211\u4eec\u4e0a\u6d77\u6676\u8bfa\u751f\u7269\u79d1\u6280\u6709\u9650\u516c\u53f8<\/p>\n<p>\u7535\u8bdd\uff1a0757-29328131-616<\/p>\n<p>\u624b\u673a\/\u5fae\u4fe1\uff1a13392273325<\/p>\n<p>Q Q\uff1a2775427010<\/p>\n<p>\u4e1a\u52a1\uff1ainfo@gene-optimal.com<\/p>\n<p>\u62db\u8058\uff1ahr@gene-optimal.com<\/p>\n<p>\u5730\u5740\uff1a\u5e7f\u4e1c\u7701\u4f5b\u5c71\u5e02\u4f5b\u5c71\u65b0\u57ce\u5cad\u5357\u5927\u90532\u53f7\u4e2d\u6b27\u4e2d\u5fc3A\u680b<\/p>\n<p>\u5b98\u7f51\uff1awww.gene-optimal.com<\/p>\n<p>&nbsp;<\/p>\n<p><span style=\"background-color: #ebf5ff; color: #3366ff; font-size: 10pt;\">\u7248\u6743\u58f0\u660e\uff1a\u672c\u6587\u7cfb\u6676\u8bfa\u751f\u7269\u7f16\u8bd1\u6574\u7406\uff0c\u672a\u7ecf\u6676\u8bfa\u751f\u7269\u6388\u6743\uff0c\u4e0d\u5f97\u8f6c\u8f7d\u548c\u4f7f\u7528\uff0c\u5982\u9700\u83b7\u53d6\u6388\u6743\uff0c\u8bf7\u8054\u7cfb\u6211\u4eec\u3002<\/span><\/p>\n<\/section>\n","protected":false},"excerpt":{"rendered":"<p>\u76ee\u524d\uff0c\u8010\u836f\u7ed3\u6838\u83cc\u7684\u4f20\u64ad\u4e0e\u8513\u5ef6\u5df2\u6210\u4e3a\u7ed3\u6838\u75c5\u63a7\u5236\u7684\u96be\u9898&#8230;<\/p>\n","protected":false},"author":1,"featured_media":41617,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[4],"tags":[],"_links":{"self":[{"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/posts\/41601"}],"collection":[{"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/comments?post=41601"}],"version-history":[{"count":0,"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/posts\/41601\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/media\/41617"}],"wp:attachment":[{"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/media?parent=41601"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/categories?post=41601"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/tags?post=41601"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}