{"id":41523,"date":"2019-08-13T17:13:58","date_gmt":"2019-08-13T09:13:58","guid":{"rendered":"https:\/\/www.gene-optimal.com\/?p=41523"},"modified":"2019-09-09T15:04:46","modified_gmt":"2019-09-09T07:04:46","slug":"tb-proteomic-analysis-1","status":"publish","type":"post","link":"https:\/\/www.gene-optimal.com\/tb-proteomic-analysis-1\/","title":{"rendered":"\u86cb\u767d\u8d28\u7ec4\u5b66\u5e94\u7528\u4e8e\u7ed3\u6838\u7814\u7a76-\u5b9a\u91cf\u5206\u6790\u7bc7"},"content":{"rendered":"

\u5173\u6ce8\u6676\u8bfa\u751f\u7269\u7684\u670b\u53cb\u4eec\u5e94\u8be5\u5bf9\u6211\u4eec\u7684\u201c\u62db\u724c\u201d\u2014\u2014\u7ed3\u6838\u5206\u679d\u6746\u83cc\u57fa\u56e0\u6572\u9664\u6280\u672f\u670d\u52a1\u6bd4\u8f83\u719f\u6089\u4e86\uff0c\u4e5f\u5bf9\u63a5\u7740\u63a8\u51fa\u7684\u7ed3\u6838\u5206\u679d\u6746\u83cc\u5168\u57fa\u56e0\u7ec4\u6d4b\u5e8f\u548c\u7ed3\u6838\u5206\u679d\u6746\u83cc\u91cd\u8981\u514d\u75ab\u539f\u86cb\u767d\u4ea7\u54c1\u6709\u4e00\u5b9a\u7684\u4e86\u89e3\u3002\u73b0\u5728\uff0c\u7ed3\u6838\u5206\u679d\u6746\u83cc\u86cb\u767d\u8d28\u7ec4\u5b66\u68c0\u6d4b\u5206\u6790\u670d\u52a1\u4e5f\u8ddf\u5927\u5bb6\u89c1\u9762\u5566\uff01\u63a5\u4e0b\u6765\u5e26\u5927\u5bb6\u4e86\u89e3\u4e00\u4e0b\u86cb\u767d\u8d28\u7ec4\u5b66\u4ee5\u53ca\u86cb\u767d\u8d28\u7ec4\u5b66\u5b9a\u91cf\u5206\u6790\u9488\u5bf9\u4e8e\u7ed3\u6838\u7684\u5e94\u7528\u3002<\/span><\/p>\n

 <\/p>\n

\u4e00\u3001\u86cb\u767d\u8d28\u7ec4\u5b66\u4ecb\u7ecd<\/span><\/strong><\/p>\n

 <\/p>\n

1. \u86cb\u767d\u7ec4\u5b66<\/strong><\/p>\n

\u4ece\u6574\u4f53\u7684\u89d2\u5ea6\uff0c\u5206\u6790\u7ec6\u80de\u5185\u52a8\u6001\u7684\u86cb\u767d\u8d28\u7ec4\u5206\u6210\u5206\u3001\u8868\u8fbe\u6c34\u5e73\u4e0e\u4fee\u9970\u72b6\u6001\uff0c\u4e86\u89e3\u86cb\u767d\u8d28\u4e4b\u95f4\u7684\u4e92\u4f5c\u4e0e\u8054\u7cfb\uff0c\u662f\u63ed\u793a\u86cb\u767d\u8d28\u529f\u80fd\u4e0e\u7ec6\u80de\u751f\u547d\u6d3b\u52a8\u89c4\u5f8b\u7684\u4e00\u95e8\u65b0\u7684\u79d1\u5b66\u6280\u672f\u3002<\/p>\n

2. \u5b9e\u9a8c\u6d41\u7a0b<\/strong><\/p>\n

\u52a0\u6807\u7b7e\uff1a<\/span>\u6837\u54c1\u2014\u86cb\u767d\u8d28\u63d0\u53d6\u2014\u9176\u89e3\u2014\u52a0\u6807\u7b7e\u2014\u6df7\u5408\u4e0a\u673a\u2014\u8d28\u8c31\u5b9a\u91cf\u2014\u751f\u7269\u4fe1\u606f\u5b66\u5206\u6790\u3002<\/p>\n

Lable-free\uff1a<\/span>\u6837\u54c1\u2014\u86cb\u767d\u8d28\u63d0\u53d6\u2014\u9176\u89e3-\u5355\u4e2a\u6837\u54c1\u4e0a\u673a\u2014\u8d28\u8c31\u5b9a\u91cf\u2014\u751f\u7269\u4fe1\u606f\u5b66\u5206\u6790\u3002<\/p>\n

3. \u5e38\u7528\u5b9a\u91cf\u68c0\u6d4b\u65b9\u6cd5<\/strong><\/p>\n

iTRAQ\/TMT\u6807\u7b7e\u5b9a\u91cf\uff1a<\/span>\u7ecf\u5178\u4e3b\u6d41\u6280\u672f\uff0c\u662f\u5dee\u5f02\u86cb\u767d\u8d28\u5b9a\u91cf\u5206\u6790\u65b9\u6cd5\u4e2d\u901a\u91cf\u6700\u9ad8\u3001\u7cfb\u7edf\u8bef\u5dee\u6700\u5c0f\u3001\u529f\u80fd\u5206\u6790\u6700\u5f3a\u5927\u7684\u86cb\u767d\u7ec4\u5b66\u5206\u6790\u65b9\u6cd5\u4e4b\u4e00\uff0c\u4e00\u6b21\u8d28\u8c31\u4e0a\u673a\u53ef\u540c\u65f6\u5bf92-10\u4e2a\u6837\u672c\u8fdb\u884c\u68c0\u6d4b\u548c\u6bd4\u8f83\u86cb\u767d\u8868\u8fbe\u5dee\u5f02\u3002<\/p>\n

Label-free\u5b9a\u91cf\uff1a<\/span>\u662f\u4e00\u79cd\u975e\u6807\u8bb0\u5b9a\u91cf\u65b9\u6cd5\uff0c\u7528\u6765\u6bd4\u8f83\u7684\u6837\u54c1\u4e0d\u7ecf\u8fc7\u4efb\u4f55\u6807\u8bb0\uff0c\u76f4\u63a5\u9176\u89e3\u540e\u7ecf\u8fc7\u8d28\u8c31\u4ea7\u751f\u6570\u636e\uff0c\u901a\u8fc7\u8f6f\u4ef6\u5bf9\u8c31\u56fe\u8fdb\u884c\u5f52\u4e00\u5316\u540e\uff0c\u6bd4\u8f83\u5bf9\u5e94\u8d28\u8c31\u5cf0\u7684\u5cf0\u5f3a\u5ea6\uff0c\u5cf0\u9762\u79ef\u7b49\u4e00\u7cfb\u5217\u56e0\u7d20\uff0c\u6765\u786e\u5b9a\u86cb\u767d\u8d28\u5728\u51e0\u79cd\u6837\u672c\u4e2d\u7684\u76f8\u5bf9\u8868\u8fbe\u91cf\u53d8\u5316\u3002<\/p>\n

4. \u4fe1\u606f\u5206\u6790\u5185\u5bb9<\/strong><\/p>\n

\u86cb\u767d\u8d28\u9274\u5b9a\u7ed3\u679c\u3001\u86cb\u767d\u8d28\u5b9a\u91cf\u7ed3\u679c\u3001\u86cb\u767d\u8d28GO\u529f\u80fd\u5206\u7c7b\u5bcc\u96c6\u3001\u5dee\u5f02\u86cb\u767dGO\u5bcc\u96c6\u5206\u6790\u3001\u5dee\u5f02\u57fa\u56e0\u5217\u8868\u53ca\u6570\u76ee\u6bd4\u8f83\u8868\u3001\u4e0a\u4e0b\u8c03\u57fa\u56e0\u8868\u53ca\u6570\u76ee\u6bd4\u8f83\u8868\u3001\u9274\u5b9a\u86cb\u767d\u7406\u5316\u6027\u8d28\u8868\u3001\u5dee\u5f02\u57fa\u56e0KEGG pathway\u901a\u8def\u56fe\u3001\u7f51\u7edc\u4e92\u4f5c\u56fe\u7b49\u3002<\/p>\n

 <\/p>\n

\u4e8c\u3001\u6587\u732e\u89e3\u8bfb<\/strong>\u2014M.tb<\/em> \u4fb5\u67d3\u4eba\u5de8\u566c\u7ec6\u80de\u5f15\u8d77\u5bbf\u4e3b\u53cd\u5e94\u7684\u5b9a\u91cf\u86cb\u767d\u7ec4\u5b66\u5206\u6790<\/span><\/p>\n

 <\/p>\n

<\/p>\n

1. \u5b9e\u9a8c\u5185\u5bb9<\/strong>
\n\u68c0\u6d4b\u7ed3\u6838\u5206\u679d\u6746\u83cc\u975e\u6bd2\u6027\u682aH37Ra\u3001\u6bd2\u6027\u682aH37Rv\u5206\u522b\u4fb5\u67d3\u5de8\u566c\u7ec6\u80de\u540e\uff0c\u5de8\u566c\u7ec6\u80de\u86cb\u767d\u8d28\u8868\u8fbe\u6c34\u5e73\u53d8\u5316\uff0c\u8d28\u8c31\u5b9a\u91cf\u5dee\u5f02\u86cb\u767d\uff0c\u901a\u8fc7\u751f\u7269\u4fe1\u606f\u5b66\u5206\u6790\uff0c\u5bf9\u5dee\u5f02\u86cb\u767d\u8d28\u529f\u80fd\u5206\u7c7b\u5206\u6790\u3002\u8fd9\u4e9b\u6570\u636e\u53ef\u4ee5\u66f4\u597d\u5730\u4e86\u89e3\u5bbf\u4e3b\u5185M.tb\u7684\u53d1\u75c5\u673a\u5236\uff0c\u6709\u52a9\u4e8e\u7ed3\u6838\u75c5\u7684\u9884\u9632\u548c\u4e34\u5e8a\u6cbb\u7597\u3002<\/p>\n

\u5b9e\u9a8c\u6837\u54c1<\/span><\/p>\n

THP-1\u5de8\u566c\u7ec6\u80de\uff08\u7a7a\u767d\u5bf9\u7167\uff09\u3001\u4e73\u80f6\u5fae\u7c92beads\uff08\u9634\u6027\u5bf9\u7167\uff09\u3001\u975e\u6bd2\u6027\u682aH37Ra\u548c\u6bd2\u6027\u682aH37Rv\u5206\u522b\u4fb5\u67d3THP-1\u5de8\u566c\u7ec6\u80de\u3002<\/p>\n

\u4fb5\u67d3\u540e\u5de8\u566c\u7ec6\u80de\u57f9\u517b12 h<\/span><\/p>\n

\u4f9d\u636e\uff1a\u4f7f\u7528qRT PCR\u786e\u5b9a\u88abM.tb\u4fb5\u67d3\u7684\u5de8\u566c\u7ec6\u80de\u5728\u4e0d\u540c\u65f6\u95f4\u70b9\u51cb\u4ea1\u57fa\u56e0\uff08blf-1\uff09\u6297\u51cb\u4ea1\u57fa\u56e0\uff08tnf-a\uff09\u7684\u8868\u8fbe\u6c34\u5e73\uff0c\u5b9e\u9a8c\u8868\u660e12\u5c0f\u65f6\u65f6\u4e24\u4e2a\u57fa\u56e0\u7684\u8868\u8fbe\u6c34\u5e73\u8f83\u5176\u4ed6\u65f6\u95f4\u70b9\u9ad8\u3002<\/p>\n

\u6807\u7b7e\uff1aTMT lable<\/span><\/p>\n

THP-1\u5de8\u566c\u7ec6\u80deTMT-126\u3001\u4e73\u80f6\u5fae\u7c92beadsTMT-127\u3001\u65e0\u6bd2H37RaTMT-128\u3001\u6709\u6bd2H37Rv TMT-129\u3002<\/p>\n

2. \u5b9e\u9a8c\u6d41\u7a0b<\/strong><\/p>\n

<\/p>\n

\u5b9e\u9a8c\u6d41\u7a0b\u56fe<\/span><\/p>\n

THP-1\u7ec6\u80de\u6fc0\u6d3b\u2014THP-1\u5de8\u566c\u7ec6\u80de\u611f\u67d3\u2014\u611f\u67d3\u540e\u57f9\u517b12\u5c0f\u65f6\u2014\u86cb\u767d\u8d28\u63d0\u53d6\u53ca\u5904\u7406\u2014\u52a0TMT\u6807\u7b7e\u2014\u6df7\u5408\u4e0a\u673a\uff08LC-MS\uff09\u2014\u751f\u7269\u4fe1\u606f\u5b66\u5206\u6790\u3002<\/p>\n

3. \u86cb\u767d\u8d28\u7ec4\u5b66\u5206\u6790\u7ed3\u679c<\/strong><\/p>\n

TMT\u6807\u7b7e\u86cb\u767d\u8d28\u5b9a\u91cf\u5206\u6790\uff1a\u5c06\u5dee\u5f02\u8868\u8fbe\u86cb\u767d\uff08DEPs\uff09\u6bd4\u8f83\u5206\u4e3a4\u7ec4\uff0cbeads\/THP-1\u3001H37Ra\/beads\u3001H37Ra\/beads\u3001H37Rv \/H37Ra group\uff0c\u7ed3\u679c\u5982\u4e0b\u56fe\u6240\u793a\u3002<\/p>\n

\"\"<\/p>\n

\u5dee\u5f02\u86cb\u767d\u8868\u8fbe\u805a\u7c7b\u5206\u6790<\/span><\/p>\n

beads\/THP-1\uff1a<\/span>DEPs\u4e3b\u8981\u662f\u4e0e\u81ea\u566c\u6eb6\u9176\u4f53\u548c\u514d\u75ab\u76f8\u5173\u86cb\u767d\u5982\uff1aLIMP II\u3001CD63\u3001\u7ec4\u7ec7\u86cb\u767d\u9176G\u3002\u7531\u4e8e\u4e73\u80f6\u5fae\u7c92\u751f\u7406\u523a\u6fc0\uff0c\u5bfc\u81f4\u8fdf\u53d1\u6027\u5e94\u6fc0\u53cd\u5e94\u4ea7\u751f\uff0c\u4ece\u800c\u5f15\u53d1\u514d\u75ab\u53cd\u5e94\u53ca\u541e\u566c\u4f5c\u7528\u3002<\/strong><\/p>\n

H37Ra\u3001H37Rv\u83cc\u682a\u611f\u67d3\u5de8\u566c\u7ec6\u80de\u540eI\u578b\u5e72\u6270\u7d20\u8bf1\u5bfc\u86cb\u767d\u7684\u8868\u8fbe\u91cf\u63d0\u9ad8\uff0c\u5982ISG15\u3001ISG20\u3001IFIT 1\u3001IFIT 2\u3001IFIT 3\u3001IFIH 1\u3001BST2\u3001IRF9\u3001RSAD2\uff0c\u529f\u80fd\u6ce8\u91ca\u4e3a\u514d\u75ab\u548c\u75c5\u539f\u81f4\u6b7b\u76f8\u5173\u3002<\/strong><\/p>\n

H37Rv \/H37Ra\uff1a<\/span>\u5dee\u5f02\u86cb\u767d\u8d28\u805a\u7c7b\u5206\u6790TOP 5\u5206\u522b\u4e3a\u6c27\u5316\u8fd8\u539f\u9176\u3001\u4fe1\u53f7\u5206\u5b50\u3001\u6c34\u89e3\u9176\u3001\u9176\u8c03\u5236\u5668\u3001\u6838\u9178\u7ed3\u6838\u76f8\u5173\u3002\u4e0eH37Ra\u83cc\u682a\u611f\u67d3\u6bd4\u8f83\uff0cH37Rv\u83cc\u682a\u611f\u67d3\u86cb\u767d\u8868\u8fbe\u53d1\u751f\u6539\u53d8\u6700\u660e\u663e\u7684\u662f\u8c03\u4ea1\u8fc7\u7a0b\u3001\u8840\u51dd\u3001\u6838\u5c0f\u4f53\u7ec4\u88c5\u3001\u6c27\u5316\u78f7\u9178\u5316\u3001\u56ca\u6ce1\u76f8\u5173\u86cb\u767d<\/strong>\u3002\u5176\u4e2d23\u4e2a\u86cb\u767d\u4e0e\u8840\u51dd\u76f8\u5173\uff0c26\u4e2a\u86cb\u767d\u4e0e\u51cb\u4ea1\u8fc7\u7a0b\u76f8\u5173\uff0c19\u4e2a\u86cb\u767d\u4e0e\u6c27\u5316\u78f7\u9178\u5316\u76f8\u5173\uff0c\u5176\u4ed6\u4e0e\u56ca\u6ce1\u3001\u6838\u5c0f\u4f53\u7ec4\u88c5\u76f8\u5173\u3002\u6700\u4e3a\u663e\u8457\u7684\u662f\u51cb\u4ea1\u8fc7\u7a0b\u4e0e\u708e\u75c7\u53cd\u5e94<\/strong>\u3002\u5176\u4e2d\u6700\u4e3a\u660e\u663e\u7684\u86cb\u767d\u662f\uff1aAHSG\u3001IL-1B<\/strong>\u3002<\/p>\n

\u86cb\u767d\u514d\u75ab\u5370\u8ff9\uff1a<\/span>\u4e0eH37Ra\u83cc\u682a\u611f\u67d3\u5de8\u566c\u7ec6\u80de\u86cb\u767d\u8d28\u8868\u8fbe\u6c34\u5e73\u76f8\u6bd4\uff0cH37Rv\u83cc\u682a\u611f\u67d3\u5de8\u566c\u7ec6\u80de\u540eIL-1B\u7684\u8868\u8fbe\u6c34\u5e73\u51cf\u5c11\uff0cAHSG\u8868\u8fbe\u6c34\u5e73\u589e\u591a\u3002\u7ed3\u679c\u4e0e\u86cb\u767d\u8d28\u7ec4\u5b66\u5206\u6790\u7ed3\u679c\u4e00\u81f4\u3002<\/p>\n

\"\"<\/p>\n

\"\"<\/p>\n

\u6bd2\u6027\u7ed3\u6838\u5206\u679d\u6746\u83cc\u8bf1\u5bfc\u7684\u5de8\u566c\u7ec6\u80de\u7684\u7279\u5f02\u6027\u514d\u75ab\u53cd\u5e94<\/span><\/p>\n

\u5206\u522b\u7528\u6bd2\u6027\u682aH37Rv\u3001\u975e\u6bd2\u6027\u682aH37Ra\u4fb5\u67d3\u5de8\u566c\u7ec6\u80de\uff0c\u901a\u8fc7\u86cb\u767d\u8d28\u7ec4\u5b66\u5206\u6790\u5dee\u5f02\u86cb\u767d\uff0c\u6709\u52a9\u4e8e\u6211\u4eec\u7406\u89e3\u7ed3\u6838\u5206\u679d\u6746\u83cc\u7684\u81f4\u75c5\u673a\u7406\u3002\u8fd9\u4e9b\u7ed3\u679c\u4e3a\u8fdb\u4e00\u6b65\u7814\u7a76\u548c\u6cbb\u7597\u7ed3\u6838\u75c5\u6bd2\u611f\u67d3\u540e\u5bbf\u4e3b\u7684\u53cd\u5e94\u63d0\u4f9b\u4e86\u65b0\u7684\u7ebf\u7d22\u3002<\/p>\n

\u7ed3\u6838\u5206\u679d\u6746\u83ccH37Ra\u4e0eH37Rv\u611f\u67d3\u5de8\u566c\u7ec6\u80de\u7684\u5dee\u5f02\u86cb\u767d\u6bd4\u8f83\u5206\u6790\uff1a<\/span><\/p>\n

\uff081\uff09\u5de8\u566c\u7ec6\u80de\u80de\u5916\u8bf1\u6355\u7f51\uff08MET\uff09\u4e3b\u8981\u7531\u7ec4\u86cb\u767d\u3001DNA\u7ec4\u6210\uff0c\u5de8\u566c\u7ec6\u80de\u91ca\u653eMET\u6291\u5236\u5e76\u6740\u6b7b\u7ed3\u6838\u5206\u679d\u6746\u83cc\uff0cH37Rv\u83cc\u682a\u4fb5\u67d3\u5de8\u566c\u7ec6\u80de\u540e\uff0c\u53ef\u80fd\u8bf1\u5bfcMET\u76f8\u5173\u86cb\u767d\u7684\u4e0a\u8c03\u3002<\/p>\n

\uff082\uff09\u51dd\u8840\u4e0e\u514d\u75ab\u5b58\u5728\u4ea4\u4e92\u4f5c\u7528\uff1a\u7ea4\u7ef4\u86cb\u767d\u8c03\u63a7\u7ec6\u80de\u56e0\u5b50\u4ea7\u751f\u548c\u5de8\u566c\u7ec6\u80de\u5728\u673a\u4f53\u5185\u9ecf\u9644\uff0c\u89e3\u91ca\u4e86\u8089\u82bd\u80bf\u7684\u5f62\u6210\u3002<\/p>\n

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\u53c2\u8003\u6587\u732e\uff1a<\/span><\/p>\n

1. Hua Li, Sha Wei, Yuan Fang, et al. <\/em>Quantitative proteomic analysis of host responses triggered by Mycobacterium tuberculosis<\/em> infection in human macrophage cells. Acta Biochim Biophys Sin, 2017, 1\u201310.<\/span><\/p>\n

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Q Q\uff1a2775427010<\/p>\n

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\u62db\u8058\uff1ahr@gene-optimal.com<\/p>\n

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\u5b98\u7f51\uff1awww.gene-optimal.com<\/p>\n

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