{"id":23346,"date":"2018-05-24T01:25:33","date_gmt":"2018-05-23T17:25:33","guid":{"rendered":"http:\/\/www.gene-optimal.com\/?p=23346"},"modified":"2018-05-24T01:25:33","modified_gmt":"2018-05-23T17:25:33","slug":"protein-biology-services-protein-immunoprecipitation","status":"publish","type":"post","link":"https:\/\/www.gene-optimal.com\/protein-biology-services-protein-immunoprecipitation\/","title":{"rendered":"\u86cb\u767d\u751f\u7269\u5b66\u6280\u672f\u670d\u52a1-\u86cb\u767d\u514d\u75ab\u6c89\u6dc0"},"content":{"rendered":"[vc_row type=”in_container” full_screen_row_position=”middle” scene_position=”center” text_color=”dark” text_align=”left” overlay_strength=”0.3″ shape_divider_position=”bottom”][vc_column column_padding=”no-extra-padding” column_padding_position=”all” background_color_opacity=”1″ background_hover_color_opacity=”1″ column_shadow=”none” column_border_radius=”none” width=”1\/1″ tablet_text_alignment=”default” phone_text_alignment=”default” column_border_width=”none” column_border_style=”solid”][vc_column_text]\n

\u6280\u672f\u539f\u7406\u4e0e\u5b9e\u9a8c\u6b65\u9aa4<\/strong><\/span><\/h3>\n

\u86cb\u767d\u514d\u75ab\u6c89\u6dc0(Immunoprecipitation, IP)\u662f\u4e00\u79cd\u7814\u7a76\u86cb\u767d\u8d28\u95f4\u4ea4\u4e92\u4f5c\u7528\u7684\u751f\u7269\u6280\u672f\uff0c\u8fd9\u79cd\u6280\u672f\u662f\u5c06\u86cb\u767d\u8d28\u89c6\u4e3a\u6297\u539f\uff0c\u52a0\u5165\u76f8\u5bf9\u5e94\u7684\u6297\u4f53\u4e0e\u7ec6\u80de\u88c2\u89e3\u6db2\u6216\u8868\u8fbe\u4e0a\u6e05\u4e2d\u76f8\u5e94\u7684\u86cb\u767d\u7ed3\u5408\u540e\uff0c\u518d\u4e0e\u86cb\u767dA\/G\uff08ProteinA\/G\uff09\u6216\u4e8c\u6297\u5076\u8054\u7684agarose\u6216Sepharose\u73e0\u5b50\u5b75\u80b2\uff0c\u901a\u8fc7\u79bb\u5fc3\u5f97\u5230\u73e0\u5b50-\u86cb\u767dA\/G\u6216\u4e8c\u6297-\u6297\u4f53-\u76ee\u7684\u86cb\u767d\u590d\u5408\u7269\uff0c\u4ece\u800c\u8fbe\u5230\u5206\u79bb\u548c\u6d53\u7f29\u51fa\u7279\u5b9a\u86cb\u767d\u8d28\u7684\u76ee\u7684\u3002<\/p>\n

\u86cb\u767d\u514d\u75ab\u5171\u6c89\u6dc0(Co-Immunoprecipitation)\u662f\u4ee5\u6297\u4f53\u548c\u6297\u539f\u4e4b\u95f4\u7684\u4e13\u4e00\u6027\u4f5c\u7528\u4e3a\u57fa\u7840\u7684\u7528\u4e8e\u7814\u7a76\u86cb\u767d\u8d28\u76f8\u4e92\u4f5c\u7528\u7684\u7ecf\u5178\u65b9\u6cd5\u3002\u5f53\u7ec6\u80de\u5728\u975e\u53d8\u6027\u6761\u4ef6\u4e0b\u88ab\u88c2\u89e3\u65f6\uff0c\u5b8c\u6574\u7ec6\u80de\u5185\u5b58\u5728\u7684\u8bb8\u591a\u86cb\u767d\u8d28-\u86cb\u767d\u8d28\u95f4\u7684\u76f8\u4e92\u4f5c\u7528\u88ab\u4fdd\u7559\u4e86\u4e0b\u6765\u3002\u5f53\u7528\u9884\u5148\u56fa\u5316\u5728argarose beads\u4e0a\u7684\u86cb\u767d\u8d28A\u7684\u6297\u4f53\u514d\u75ab\u6c89\u6dc0A\u86cb\u767d\uff0c\u90a3\u4e48\u4e0eA\u86cb\u767d\u5728\u4f53\u5185\u7ed3\u5408\u7684\u86cb\u767d\u8d28B\u4e5f\u80fd\u4e00\u8d77\u6c89\u6dc0\u4e0b\u6765\u3002\u518d\u901a\u8fc7\u86cb\u767d\u53d8\u6027\u5206\u79bb\uff0c\u5bf9B\u86cb\u767d\u8fdb\u884c\u68c0\u6d4b\uff0c\u8fdb\u800c\u8bc1\u660e\u4e24\u8005\u95f4\u7684\u76f8\u4e92\u4f5c\u7528\u3002\u8fd9\u79cd\u65b9\u6cd5\u5f97\u5230\u7684\u76ee\u7684\u86cb\u767d\u662f\u5728\u7ec6\u80de\u5185\u4e0e\u5174\u8da3\u86cb\u767d\u5929\u7136\u7ed3\u5408\u7684\uff0c\u7b26\u5408\u4f53\u5185\u5b9e\u9645\u60c5\u51b5\uff0c\u5f97\u5230\u7684\u7ed3\u679c\u53ef\u4fe1\u5ea6\u9ad8\u3002[\/vc_column_text][\/vc_column][\/vc_row][vc_row type=”in_container” full_screen_row_position=”middle” scene_position=”center” text_color=”dark” text_align=”left” overlay_strength=”0.3″ shape_divider_position=”bottom”][vc_column column_padding=”no-extra-padding” column_padding_position=”all” background_color_opacity=”1″ background_hover_color_opacity=”1″ column_shadow=”none” column_border_radius=”none” width=”1\/1″ tablet_text_alignment=”default” phone_text_alignment=”default” column_border_width=”none” column_border_style=”solid”]

\"\"<\/div><\/div>[\/vc_column][\/vc_row][vc_row type=”in_container” full_screen_row_position=”middle” scene_position=”center” text_color=”dark” text_align=”left” overlay_strength=”0.3″ shape_divider_position=”bottom”][vc_column column_padding=”no-extra-padding” column_padding_position=”all” background_color_opacity=”1″ background_hover_color_opacity=”1″ column_shadow=”none” column_border_radius=”none” width=”1\/1″ tablet_text_alignment=”default” phone_text_alignment=”default” column_border_width=”none” column_border_style=”solid”][vc_column_text]\n

\u670d\u52a1\u6d41\u7a0b<\/strong><\/span><\/h3>\n
    \n
  1. \u5ba2\u6237\u63d0\u4f9b\u8840\u6db2\u3001\u7ec6\u80de\u3001\u7ec4\u7ec7\u7b49\u6837\u54c1<\/li>\n
  2. \u6837\u54c1\u88c2\u89e3\uff0c\u79bb\u5fc3\u540e\u6536\u96c6\u4e0a\u6e05<\/li>\n
  3. \u52a0\u5165\u76ee\u7684\u86cb\u767dA\u6297\u4f53\uff0c\u5b75\u80b2\u4e0e\u76ee\u7684\u86cb\u767dA\u7ed3\u5408<\/li>\n
  4. \u52a0\u5165\u8fde\u63a5\u6709proteinA\u6216G\u7684\u743c\u8102\u7cd6\u51dd\u80f6\u73e0\uff0c\u5b75\u80b2\u4e0e\u6297\u4f53\u7ed3\u5408<\/li>\n
  5. \u79bb\u5fc3\uff0c\u5f03\u6389\u4e0a\u6e05\uff0c\u6c89\u5e95\u8fdb\u884cSDS-PAGE\u9a8c\u8bc1<\/li>\n
  6. \u63d0\u4ea4\u7ed3\u679c\uff1a\u6297\u4f53\u6c89\u6dc0\u86cb\u767d\u3001\u86cb\u767dSDS-PAGE\u53cawestern blot\u9a8c\u8bc1\u56fe<\/li>\n<\/ol>\n[\/vc_column_text][\/vc_column][\/vc_row]\n","protected":false},"excerpt":{"rendered":"

    [vc_row type=”in_co…<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[5],"tags":[],"_links":{"self":[{"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/posts\/23346"}],"collection":[{"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/comments?post=23346"}],"version-history":[{"count":0,"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/posts\/23346\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/media?parent=23346"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/categories?post=23346"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/tags?post=23346"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}